Shear induced diffusion (SID) based flow segregation is a technique that can be used for concentration and fractionation purposes, and it has the potential to become an economical and sustainable alternative for e.g., membrane separation. When compared to conventional microfiltration, problems related to fouling and cleaning are expected to be minimal. To make the best use of the opportunities that this technique holds, detailed insights in flow and particle behavior are needed. Modelling this process allows for us to chart particle segregation in flow, as well as the effect of suspension removal through a pore and the restoration of the flow profile after the pore. As a starting point, we take the computation fluid dynamics (CFD) model that is presented in a previous study. A difference in channel height to particle diameter ratio influences the entrance length of the SID profile as well as its fully developed profile. When extracting liquid through one pore, particles are systematically transmitted at a lower concentration (59-78%) than is present in the bulk. The recovery lengths of the SID profile after the pore were short, and thus pores can be placed at realistic distances, which forms a good foundation for further design of this novel separation technology that will ultimately be applied for fractionation of particles taking relatively small differences in diffusive behavior as a starting point.