Flow-injection amperometric system for enzyme immunoassay

Ivnitskii, D. M.; Sitdikov, Ravil; Курочкин, В. Е.

doi:10.1016/0003-2670(92)80174-6

Cited by 29 publications

(2 citation statements)

References 9 publications

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“…With flow injection analysis (FIA), analytical procedures can be automated easily and the analyses performed quickly and continuously ,, . Flow injection immunoassay (FIIA) techniques have been reported extensively. − They are based mainly on immunoreactors filled by immunologically modified solid particles, gels, CPGs, etc.…”

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confidence: 99%

Flow Injection Immunoanalysis Based on a Magnetoimmunosensor System

et al. 1998

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A new immunosensor integrated to a flow system has been developed. It is based on magnetic immunoparticles immobilized on a solid-state transducer using a magnetic field. The described technique renews the immunoparticles reproducibly for each analysis allowing a good measurement precision. The developed experimental approach permits the implementation of an automated immunoassay that is quick (analytical cycle < 30 min) and sensitive in the micromolar concentration range. The system was applied to the determination of rabbit immunoglobulin G as an analyte model.

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confidence: 99%

Flow Injection Immunoanalysis Based on a Magnetoimmunosensor System

et al. 1998

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“…The reaction was started by addition of sodium iodide to a final concentration of 1 mM [17]. Horseradish peroxidase (the enzyme label) catalyzes oxidation of iodide in the presence of H202 resulting in formation of iodine [21]. The amperometric output was obtained as a steady-state current of the formed iodine electroreduction [ 171.…”

Section: Incubation (2 Minmentioning

confidence: 99%

Fast amperometric immunoassay for hantavirus infection

et al. 1996

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The determination of specific antibodies against Hantavirus in blood plasma has been developed. The assay technique is based on a flow through amperometric immunoelectrode. Highly dispersed carbon material serves as an immunosorbent and at the same time as an electrode material. The analysis employed the 'sandwich' assay scheme. Recombinant protein of Hantavirus was immobilized on the surface of the immunosorbent. Target analyte captured by the immunosorbent at the first stage of incubation interacts with the peroxidase labeled antihuman antibodies at the second stage of incubation. The detection of peroxidase label was conducted amperometrically by electroreduction of iodine formed as a product of peroxidase catalyzed reaction. The technique permits determination of specific antibodies against Hantavirus in blood plasma using a 22min assay time.

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Portable Immunosensor for the Fast Amperometric Detection of Anti-Hantavirus Antibodies

Vetcha¹,

Abdel-Hamid²,

Атанасов³

et al. 2000

Electroanalysis

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An amperometric immunosensor based on a recombinant nucleocapsid antigen has been developed for fast determination of antibodies against Sin Nombre (SN) virus in human blood serum. This approach combines the advantages of utilization of highly dispersed immunosorbent, with highly sensitive electrochemical detection of enzyme‐marker, and flow‐injection scheme of immunoassay. A main benefit of recombinant nucleocapsid antigen is that antigen preparation and standardization is easy. The complete immunoassay can be performed in 24 minutes. The short overall assay time could be a major advantage in the field of diagnostic screening of infectious diseases in mobile laboratories and small hospitals. The immunosensor has been successfully used in the determination of anti‐Hantavirus IgG in human blood plasma and is easily adaptable for the detection of other types of viral infections.

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Flow-injection amperometric system for enzyme immunoassay

Cited by 29 publications

References 9 publications

Flow Injection Immunoanalysis Based on a Magnetoimmunosensor System

Flow Injection Immunoanalysis Based on a Magnetoimmunosensor System

Fast amperometric immunoassay for hantavirus infection

Portable Immunosensor for the Fast Amperometric Detection of Anti-Hantavirus Antibodies

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