Flow injection analysis of ethambutol in synthetic urine using a graphite-polyurethane composite electrode as an amperometric detector

Perantoni, Camila B.; Azevedo, Alessandra B. R. de; Vaz, Fernando Antônio Simas; Oliveira, Marcone Augusto Leal de; Matos, Renato Camargo; Lowinsohn, Denise

doi:10.2478/s11532-013-0301-9

Cited by 1 publication

(1 citation statement)

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“…Shao et al applied a MWCNT modified glassy carbon electrode to the detection of this drug in pharmaceutical formulations with a LOD of 7.6 × 10 −4 mM [ 46 ]. Perantoni et al reported two graphite-based electrodes for the amperometric detection of ETB in pharmaceutical formulations [ 41 ] and in synthetic urine [ 47 ] with detection limits of 0.1 mM and 0.0634 mM, respectively. A biosensor based on the use of silver nanoparticles was developed by Ngece et al, exhibiting a LOD of 7.0 × 10 −4 mM ETB in serum samples [ 40 ].…”

Section: Resultsmentioning

confidence: 99%

Development of a Nafion/MWCNT-SPCE-Based Portable Sensor for the Voltammetric Analysis of the Anti-Tuberculosis Drug Ethambutol

Couto

Quinaz

2016

Sensors

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Herein we describe the development, characterization and application of an electrochemical sensor based on the use of Nafion/MWCNT-modified screen-printed carbon electrodes (SPCEs) for the voltammetric detection of the anti-tuberculosis (anti-TB) drug ethambutol (ETB). The electrochemical behaviour of the drug at the surface of the developed Nafion/MWCNT-SPCEs was studied through cyclic voltammetry (CV) and square wave voltammetry (SWV) techniques. Electrochemical impedance spectroscopy (EIS) and scanning electron microscopy (SEM) were employed to characterize the modified surface of the electrodes. Results showed that, compared to both unmodified and MWCNTs-modified SPCEs, negatively charged Nafion/MWCNT-SPCEs remarkably enhanced the electrochemical sensitivity and selectivity for ETB due to the synergistic effect of the electrostatic interaction between cationic ETB molecules and negatively charged Nafion polymer and the inherent electrocatalytic properties of both MWCNTs and Nafion. Nafion/MWCNT-SPCEs provided excellent biocompatibility, good electrical conductivity, low electrochemical interferences and a high signal-to-noise ratio, providing excellent performance towards ETB quantification in microvolumes of human urine and human blood serum samples. The outcomes of this paper confirm that the Nafion/MWCNT-SPCE-based device could be a potential candidate for the development of a low-cost, yet reliable and efficient electrochemical portable sensor for the low-level detection of this antimycobacterial drug in biological samples.

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Section: Resultsmentioning

confidence: 99%