Flow Injection Analysis of Nitrite Based on Spectrophotometric Measurements of Iodine Formed by Oxidation of Iodide with Nitrite

Miura, Yasuyuki; Kusakari, Katsumi

doi:10.2116/analsci.15.923

Cited by 16 publications

(6 citation statements)

References 19 publications

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“…An additional human threat posed by the presence of nitrite at high concentration in the blood is its reaction with iron (III) of the haemoglobin, forming methemoglobin, which has no oxygen carrying ability 2 . Many analytical methods have been proposed for the detection of nitrite [2][3][4][5][6][7][8][9][10] . Most of the methods employed for the determination of nitrite are based on the colorimetric determination of nitrite with diazocoupling reaction.…”

Section: Introductionmentioning

confidence: 99%

Detection of nitrite by flow injection analysis using a novel paired emitter-detector diode (PEDD) as a photometric detector

et al. 2007

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An inexpensive flow injection analysis system for determining low concentration levels of nitrite employing the Griess reagent spectrophotometric method is reported. The novel photometric detector applied within this manifold is a highly sensitive, low cost, miniaturized light emitting diode (LED) based flow detector. This colorimetric detector employs two LEDs, operating one as a light source and the other as a light detector. The emitter LED is forward biased and the detector reverse biased. The emitter and detector LED had a λ max of 530 nm and 623 nm respectively. The emission spectrum of the emitter LED efficiently overlapped with the absorbance spectrum of 9 µM NO 2 and Griess reagent complex. A simple timer circuit measures the time taken for the photocurrent generated by the emitter LED to discharge the detector LED from 5 V (logic 1) to 1.7 V (logic 0). The Griess reagent method employed for nitrite determination is based on the formation of an azo dye, the intensity of which is directly related to nitrite concentration. The linear range, reproducibility and limit of detection were investigated. Detection limits in the nanomolar range were achieved using the Paired Emitter-Detector Diode (PEDD) flow analysis device. For a comparative study the linear range and limit of detection were also investigated using a platewell reader. Higher sensitivity and improved precision were obtained from the PEDD compared to the commercially available plate well reader.

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Section: Introductionmentioning

confidence: 99%

Detection of nitrite by flow injection analysis using a novel paired emitter-detector diode (PEDD) as a photometric detector

et al. 2007

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“…We proposed direct and indirect methods of determination of various forms of iodine on the PUF surface by the SPSP method [11,12]. Detection of iodine being formed at oxida tion by iodine nitrite is used for spectrophotometric [13], amperometric [14], ionochromatographic [15,16], and chemiluminescent [ [17] determination of nitrite.…”

Section: Introductionmentioning

confidence: 99%

“…EXPERIMENTAL All reagents were of "chemically pure" qualification and they were used without additional purification. Standard solution, 0.01 mol/dm 3 , of nitrite was prepared by dissolving 0.3450 g of sodium nitrite in ~400 cm 3 of water, then 50 cm 3 0.1 mol/dm 3 of sodium hydroxide was added as nitrite stabilizer [13] and diluted with water to 500 cm 3 . When the standard nitrite solution was stored at room temperature in a dark place its con centration for 6 month of storage after preparation did not change.…”

Section: Introductionmentioning

confidence: 99%

Iodometric solidphase spectrophotometric determination of nitrite with the use of polyurethane foam as a sorbent

Тrohimenko¹,

Zaporozhets²

2014

J. Water Chem. Technol.

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The article contains the developed technique of iodometric solidphase spectrophotometric determination of nitrite in natural water using polyurethane foam as a sorbent. The redox reaction between nitrite and iodide, sorption of formed iodine by polyurethane foam and its detection on the surface of the sorbent lie behind the technique. Linearity of the graduation graph is observed until the nitrite concentra tion up to 0.5 mg/dm 3 , the detection limit is 0.006 mg/dm 3 at the sample volume 10 cm 3 .

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“…Various methods have been used for the determination of nitrite, including chromatography, 4-7 gas chromatography-mass spectrometry, 8 capillary electrophoresis, [9][10][11] colorimetric methods, [12][13][14][15][16][17][18][19][20][21][22][23] catalytic-based methods, [24][25][26] and several other methods such as electrochemical ones, 27-29 fluorometric ones 30,31 and chemiluminescence. [32][33][34] Flow injection analysis (FIA) in combination with one of the above detection methods have been widely used for the determination of nitrite.…”

Section: Introductionmentioning

confidence: 99%

A Simple Flow Injection Spectrophotometric Determination of Nitrite Based on Its Reaction with Thiourea

2005

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A simple flow injection spectrophotometric method for the determination of nitrite is described. Nitrite injected into the flow system reacts with thiourea in acidic medium and the generated thiocyanate ion reacts with Fe(III) in the reagent solution to produce a highly colored product. The influences of chemical and physical parameters including reagent concentrations, sample volume injected, flow rates of the carrier and reagent solutions, reaction coil length and reaction temperature, were studied and optimum values of these parameters were established. Under the optimum conditions, the calibration curve for nitrite was linear over the concentration range 0.36 -90 µg ml -1 without preconcentration and over the range 3.8 -500 ng ml -1 with a simple online preconcentration step using an anion exchange column. The corresponding detection limits were 0.36 µg ml -1 and 3.8 ng ml -1 , respectively. Up to 25 samples can be analyzed per hour, with an average relative standard deviation of ≤ 1.2%. Interferences by various foreign ions were studied and the method was applied to the determination of nitrite in water and spiked water samples.

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Flow Injection Analysis of Nitrite Based on Spectrophotometric Measurements of Iodine Formed by Oxidation of Iodide with Nitrite

Abstract: Keywords Flow injection analysis, determination of nitrite, oxidation of iodide with nitrite, photometric measurement of iodine, water analysis † To whom correspondence should be addressed.

Cited by 16 publications

References 19 publications

Detection of nitrite by flow injection analysis using a novel paired emitter-detector diode (PEDD) as a photometric detector

Detection of nitrite by flow injection analysis using a novel paired emitter-detector diode (PEDD) as a photometric detector

Iodometric solidphase spectrophotometric determination of nitrite with the use of polyurethane foam as a sorbent

A Simple Flow Injection Spectrophotometric Determination of Nitrite Based on Its Reaction with Thiourea

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