Flow Injection Analysis with Turbidity Detection for the Quantitative Determination of Mebeverine Hydrochloride in Pharmaceutical Formulations

Abstract

The main objective of this paper is to develop and validate flow injection method, a precise, accurate, simple, economic, low cost and specific turbidimetric method for the quantitative determination of mebeverine hydrochloride (MbH) in pharmaceutical preparations. A homemade NAG Dual & Solo (0-180º) analyser which contains two identical detections units (cell 1 and 2) was applied for turbidity measurements. The developed method was optimized for different chemical and physical parameters such as perception reagent concentrations, aqueous salts solutions, flow rate, the intensity of the sources light, sample volume, mixing coil and purge time. The correlation coefficients (r) of the developed method were 0.9980 and 0.9986 for cell 1 and 2 respectively and showed the linearity of response against concentration over the range of 1.0 to 6.5 and 0.7-6.5mmol/L for cell 1 & 2 respectively. The limit of detections (LOD) for cell 1 and cell 2 were 0.28 and 0.21 mmol/L respectively. The intra-day and inter-day precision for two serial estimations of 3.5 and 5.5 mmol/L of MBH exhibited a relative standard deviation of 0.46%, 0.28%, 0.23%, 0.26% and 0.39%, 0.79%, 0.14%, 0.05% for cell 1 & 2 respectively. The accuracy of the developed method has expressed a recovery percentage (Rec %) and error % which was between 99.22 to 101.13 and 99.39 to 101.17 for cell 1 and cell 2 respectively. The ICH guidelines were followed for method validation. The developed method was successfully applied for the determination of MbH in pure and pharmaceutical preparations and the method can be conveniently used for routine analysis in laboratory as a quality control method since the method permits quantitively determination of 60 samples/h.

Rapid colorimetric sensing of chlorpromazine HCl antipsychotic through in situ growth of gold nanoparticles

“…It plays a vital role in patient safety, pharmacological research, and the overall management of healthcare. Therefore, a series of methods have been developed for the determination of various drugs, including ow injection, [14][15][16] turbidity, [17][18][19] solid phase extraction, 20 HPLC, 21 electrochemistry 22 and spectrophotometry. 23,24 Inspired by these revolutionary works, a team of procient scientists and researchers has successfully developed a colorimetric assay using AuNPs, enabling the swi detection of bagonists.…”

“…It plays a vital role in patient safety, pharmacological research, and the overall management of healthcare. Therefore, a series of methods have been developed for the determination of various drugs, including flow injection, 14–16 turbidity, 17–19 solid phase extraction, 20 HPLC, 21 electrochemistry 22 and spectrophotometry. 23,24…”

Development and Validation of Spectrophotometric Methods for the Quantitative Determination of Doxycycline Hyclate in Pure Form and Pharmaceutical Formulations Using Flow‐Injection and Batch procedures: A Comparative Study

“…[16,17] Given the significant decreases in waste generation compared to previous procedures, [18,19] they are environmentally safer. [20][21][22] Numerous spectrophotometric techniques based on colourimetric reactions have been described for the detection of doxycycline hyclate in pharmaceutical preparations and biological fluids. These techniques use reagents such as Iron (III), [23] Cu (II)/H 2 O 2 , [24] bromate-bromide, [25] diazotized 4aminoacetophenone, [10] and semicarbazide.…”

“…They can also be easily automated, are less prone to contamination, and use relatively fewer reagents and sample material [16,17] . Given the significant decreases in waste generation compared to previous procedures, [18,19] they are environmentally safer [20–22] . Numerous spectrophotometric techniques based on colourimetric reactions have been described for the detection of doxycycline hyclate in pharmaceutical preparations and biological fluids.…”

Design a continuous microfluidic flow cell for turbidimetric-flow injection technology: A new approach for routine analysis of active pharmaceutical formulations

“…Moreover, the turbidimetric method has the advantages of low consumption of samples and reagents, high accuracy, simple operation and quick analysis. Therefore, several turbidimetric-FIA procedures have been developed for the determination of active pharmaceutical ingredients in different matrices [25][26][27][28][29]. To overcome the limitations associated with the spectrophotometric-flow injection technique, an inexpensive, very simple and long-optical-path turbidimetric-flow cell is designed and evaluated.…”