Flow-Injection Chemiluminescence Method for Sensitive Determination of Ascorbic Acid in Fruit Juices and Pharmaceutical Samples Using a Luminol-Cetyltrimethylammonium Chloride Reversed Micelle System

Abstract

A highly sensitive flow-injection (FI) method was developed for the determination of ascorbic acid using chemiluminescence (CL) based detection. This method involved the following processes: (1) reduction of tetrachloroaurate(III) in hydrochloric acid with ascorbic acid; (2) on-line extraction of the residual Au(III) with rhodamine B from the aqueous hydrochloric acid solution into toluene, followed by the separation of the Au(III)-containing organic phase from the aqueous phase through a microporous Teflon membrane in the flow system; and (3) the measurement of CL produced in a flow cell upon mixing of the extract stream of Au(III) in toluene with luminol in the reversed micellar medium of cetyltrimethylammonium chloride-water in 1-hexanol-cyclohexane, which was injected into a CL reagent stream. In this procedure, a reduction in the CL intensity occurred due to the addition of ascorbic acid to the Au(III) solution. The CL signal of Au(III) decreased with increasing concentration of ascorbic acid in the aqueous sample solution. The proposed procedure allowed the indirect quantitative determination of ascorbic acid in the range of 1.0 × 10 to 1.0 × 10 M with a correlation coefficient of 0.987 and relative standard deviation of 2.1% (n = 6) at 1.0 × 10 M. The proposed FI-CL methodology was successfully applied for quantitative determination of ascorbic acid in fruit juices and pharmaceutical samples.

Development of an Optode Detector for Determination of Anionic Surfactants by Flow Injection Analysis

“…19,20 Flow injection analysis (FIA) methods have the advantages of higher reproducibility, higher sample throughput, lower reagent consumption, and easier procedures than the batchwise methods. [31][32][33][34][35][36] Several FIA systems based on optode detectors have been reported. [21][22][23][24][25][26][27][28] Therefore, the combination of an FIA system and the optode detector based on L-RB is considered to be a very effective analytical method for determining AS.…”

Rhodamine B Chemiluminescence Improved by Mimetic AuCu Alloy Nanoclusters and Ultrasensitive Measurement of H2O2, Glucose and Xanthine

“…[17][18][19] CL detection methods are famous for their high sensitivity and wide dynamic linear ranges. [20][21][22][23] Nowadays, researchers are interested in using nanomaterials with unique properties to improve the CL systems. 20,21,24 Nanomaterials can play the role of emitters, catalysts or energy acceptors, improving the CL reactions and resulting in an enhanced emission.…”

Lansoprazole Determination in Pharmaceutical Formulations by Flow Injection Coupled with Acidic KMnO4-Quinine Chemiluminescence System

“…This technique is accompanied by several advantages, including high sensitivity and sample throughput, controllable emission rate and safety. 28 Numerous analytical applications of flow injection (FI) techniques with CL detection have been reported for the determination of environmentally, pharmaceutically and biologically important analytes [29][30][31][32] using a number of oxidants. 33,34 Potassium permanganate (KMnO4) as a CL reagent has been extensively used since 1975, when sulfur dioxide (SO2) was determined in atmospheric samples by developing an analytical method in which KMnO4 was employed as a CL reagent.…”