Flow‐injection determination of streptomycin residues in milk using the luminol–periodate–Mn²⁺ chemiluminescence system

Abstract

A novel flow-injection method with chemiluminescence detection was developed for the determination of streptomycin residues in milk, based on the enhancement by streptomycin of the luminol-potassium periodate-Mn(2+) chemiluminescence system. The assay allowed analysis of streptomycin residues in whole milk samples ( approximately 3.5% fat) after sample work-up procedures. The limit of detection was 5.16 x 10(-9) mol/L for milk, and was far below the regulations of maximum residue limits (MRLs). The relative standard deviation (RSD) for 8.66 x 10(-8) mol/L streptomycin was 1.94% (n = 15) in milk. The results obtained for the assay of streptomycin residues in milk was comparable with those obtained by the official methods (ELISA) and demonstrated good accuracy and precision. The possible mechanism is briefly discussed.

Determination of aminoglycoside antibiotics using an on-chip microfluidic device with chemiluminescence detection

“…However, a relatively high LOD for amikacin was obtained by using the same CL system in a conventional FI method [22]. Conventional FI methods using other luminol systems have been also described for several individual AG antibiotics [26][27][28], but the method described here is the first one which uses a microfluidic system for AG determination using CL detection.…”

“…A comparison of the figures of merit of this work with those of other methods for the determination of aminoglycoside antibiotics involving CL detection [22,[24][25][26][27][28][29][30] is shown in Table 3. As can be seen, the luminol-hydrogen peroxideCu(II) system has been described for the CL detection of amikacin alone [24] and AG mixtures [25] by liquid chromatography, reaching very low LOD values in this last method, which includes a previous preconcentration step.…”

Recent Advances in Optical Detection of Aminoglycosides

“…The CL of luminol was reported by Albrecht in 1928. Streptomycin was reported having an enhancement effect on the luminol-potassium periodate-Mn 2+ CL reaction [68]. Luminol-H 2 O 2 is one of the most investigated CL reactions.…”

A Spectrophotometric Study of Streptomycin Effect on the Clinical Urea Determination

“…[3][4][5][6] Because of its serious nephrotoxicity and ototoxicity, there is an increasing tendency to reserve the use of streptomycin only for the treatment of tuberculosis in conjunction with other drugs. 7 Streptomycin has been determined by a variety of analytical techniques, such as UV/vis spectrophotometry, [8][9][10] fluorimetry, 11,12 flow-injection with chemiluminescence detection, 13 capillary zone electrophoresis, 14,15 solid-phase extraction and liquid chromatography. 16,17 However, almost all of these methods require expensive equipment, and the conditions of conducting measurements are so restrictive that the results are greatly affected by environmental factors.…”