Flow Injection Spectrophotometric Determination of N-Acetyl-L-cysteine as a Complex with Palladium(II)

Giljanović, Josipa; Brkljača, Mia; Prkić, Ante

doi:10.3390/molecules16097224

Cited by 13 publications

(9 citation statements)

References 22 publications

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“…8. Calculation of the binding constants K for DOX-BSA complexes in the presence of different concentrations of AuNPs was performed according to the definition of such constants in (Woldbye 1955;Zhou and He 1993;Giljanović et al 2011):…”

Section: Resultsmentioning

confidence: 99%

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Understanding prodrugs: complexation in aqueous solutions of doxorubicin, bovine serum albumin and gold nanoparticles

et al. 2019

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Aqueous solutions of doxorubicin (DOX), doxorubicin with bovine serum albumin (DOX-BSA), and DOX-BSA in solutions containing gold nanoparticles (AuNPs) with different concentrations of each system component are studied for the first time. The study of the concentration and time-resolved dependences of optical density spectra indicates that the interaction of protonated molecules of the antibiotic with the citrate-covered surface of the nanoparticles of high concentration promotes the aggregation and precipitation of AuNPs. On the other hand, protein macromolecules effectively form conjugates with nanoparticles, preventing their aggregation. Competition of these processes significantly complicates the behavior of optical density spectra at the absorption band of doxorubicin. The simultaneous processes of AuNP aggregation and BSA-AuNP association allow reasonable result of calculation of equilibrium-binding constants for the DOX-BSA complexation process to be obtained only at low nanoparticle concentrations (up to 1.3 × 10 −10 M). It was found that these constants decrease to K = 1.18 × 10 6 M −1 compared with K = 4.74 × 10 6 M −1 for the DOX-BSA complex, indicating lower stability of these complexes after citrate-stabilized nanoparticles addition. The number of binding sites in DOX-BSA complexes, as shown by the analysis of the isomolar series curves, stays the same for the indicated AuNPs concentrations and is equal to n = 2. The obtained results could be useful to clarify the appropriate concentration of such type of AuNPs upon the development of oncology prodrugs.

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Section: Resultsmentioning

confidence: 99%

“…In another series, the concentrations of both DOX and BSA changed, but their total concentration remained constant according to the Ostromislensky-Job method of isomolar series (Woldbye 1955;Zhou and He 1993;Giljanović et al 2011) (Table 2).…”

Section: Methodsmentioning

confidence: 99%

Understanding prodrugs: complexation in aqueous solutions of doxorubicin, bovine serum albumin and gold nanoparticles

et al. 2019

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“…Open Access Pharmaceutica Analytica Acta means such as: reaction of thiol group with o-phthalaldehyde and isoleucine [21], complexation reaction of the drug with Pd 2+ [22] and a redox reaction employing generation of prussian blue product [23]. Chromatographic methods were also applied for the analysis of this compound.…”

Section: Research Articlementioning

confidence: 99%

Spectrofluorimetric Estimation of Some Sulfhydryl–Containing Drugs by Demasking Reaction of the Palladium Chelate of 8-Hydroxyquinoline-5-Sulfonic Acid

Rs¹,

Sf²,

Hewala³

et al. 2017

Pharm Anal Acta

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A simple and sensitive spectrofluorimetric method has been developed for the determination of some selected sulfhydryl-containing drugs namely Acetylcysteine (ACS), Captopril (CAP) and Mesna (MSN).The method is based on the interaction of the drugs with potassium (5-sulfoxino) palladium II in alkaline medium in presence of magnesium ions, where the sulfhydryl group combines with palladium from the non-fluorescent potassium bis (5-sulfoxino) palladium II. The resulting 8-hydroxy-5-quinoline sulfonic acid coordinates with magnesium to form the fluorescent chelate that is a measure of the amount of sulfhydrl containing drug analyzed. The fluorescence intensity was measured at an emission wavelength of 485 nm, by excitation at 345 nm. All the experimental parameters affecting the reaction were studied and optimized. The proposed method was applicable over the concentration range of 0.04-0.44 µg/mL for the three drugs and was applied for their determination in bulk form and in pharmaceutical preparations without interference from common excipients. The assay results were statistically compared with those obtained from previously reported methods where no significant difference was found between them. The selectivity and the stability-indicating aspect of the proposed method were confirmed by preparing the disulphides of the studied drugs and applying the reaction to the parent drugs in presence of their disulphides where no interference was detected from these related substances. By virtue of its high sensitivity, the proposed method was also extended to analyze the drugs in spiked human plasma and urine.

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“…Different methods have been reported for the determination of L-cysteine in biological samples. These include chromatography [3][4][5], electro-phoresis [6], spectrophotometry [7][8][9][10], and chemiluminescence [11]. Electroanalytical methods have attracted much attention in recent years for the determination of L-cysteine and other thiol compounds because of their high sensitivity, accuracy, low cost, and simplicity [12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Application of 3,4-dihydroxycinnamic acid as a suitable mediator and multiwall carbon nanotubes as a sensor for the electrocatalytic determination of L-cysteine

Keyvanfard

Salmani-mobarakeh

Karimi-Maleh

et al. 2014

Chinese Journal of Catalysis

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A highly sensitive electrochemical sensor was prepared for the determination of L-cysteine using a modified multiwall carbon nanotubes paste electrode in the presence of 3,4-dihydroxycinnamic acid (3,4-DHCA) as a mediator, based on an electrocatalytic process. The results indicate that the electrode is electrocatalytically efficient for the oxidation of L-cysteine in the presence of 3,4-DHCA. The interaction between the mediator and L-cysteine can be used for its sensitive and selective determination. Using chronoamperometry, the catalytic reaction rate constant was calculated to be 2.37 × 10 2 mol -1 L s -1 . The catalytic peak current was linearly dependent on the L-cysteine concentration in the range of 0.4-115 μmol/L. The detection limit obtained by linear sweep voltammetry was 0.25 μmol/L. Finally, the modified electrode was examined as a selective, simple, and precise new electrochemical sensor for the determination of L-cysteine in real samples.

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Flow Injection Spectrophotometric Determination of N-Acetyl-L-cysteine as a Complex with Palladium(II)

Cited by 13 publications

References 22 publications

Understanding prodrugs: complexation in aqueous solutions of doxorubicin, bovine serum albumin and gold nanoparticles

Understanding prodrugs: complexation in aqueous solutions of doxorubicin, bovine serum albumin and gold nanoparticles

Spectrofluorimetric Estimation of Some Sulfhydryl–Containing Drugs by Demasking Reaction of the Palladium Chelate of 8-Hydroxyquinoline-5-Sulfonic Acid

Application of 3,4-dihydroxycinnamic acid as a suitable mediator and multiwall carbon nanotubes as a sensor for the electrocatalytic determination of L-cysteine

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