Flow-Injection Spectrophotometric Determination of Sulfadiazine and Sulfamethoxazole in Pharmaceuticals and Urine

Abstract

A rapid and sensitive flow-injection spectrophotometric method is proposed for the determination of sulfadiazine and sulfamethoxazole. This method is based on the diazotization of sulfonamide with sodium nitrite, and a coupling reaction of the diazo-compound with α-naphthylamine. The optimum experimental conditions are obtained by using the controlled and weighted centroid simplex method. The linear ranges for the determination of sulfadiazine and sulfamethoxazole are 0.2 -20 µg ml -1 and 0.1 -20 µg ml -1 , and their detection limits are 0.06 µg ml -1 and 0.05 µg ml -1 , respectively, and the sampling frequency is 130 samples per hour. The method has been used to determine sulfadiazine and sulfamethoxazole in pharmaceuticals and urine without separation. The results are in agreement with those obtained by a high-performance liquid chromatograph technique at the 95% confidence level.

Validação de metodologia de doseamento de sulfametoxazol (matéria-prima) por espectrofotometria / Validation of an uv spectrophotometric method for sulfametoxazole raw material

“…v. 7, n. 1, p. 62-71, jan./jun. 2018 ISSNe 2316-347X titulação potenciométrica (EUROPEAN PHARMACOPEIA, 2005), espectrofotometria derivada (FAN et al, 2003), espectrofluorimetria (BLANCO et al,1999 e cromatografia líquida de alta eficiência (NEVADO;PEÑALVO;BERNARDO, 2001, CRUZ et al, 2010, entretanto um método simples e rápido, empregando espectrofotometria na região do ultravioleta, não foi encontrado.…”

Permanganate Oxidation of Sulfadiazine: Analytical Method Development and Kinetic Study

“…There are several ISSN Print: 2229-7928 ISSN Online: 2230-7532 methods reported for the estimation of sulfadiazine, they include HPLC [5][6][7][8][9] in addition to the UV detector Mass spectroscopic determination was also employed in the determination of sulfadiazine [10][11][12] . Spectrophotometric method was also applied for the estimation of sulfadiazine 1,13 continuous flow spectrophotometric 14 and flow injection method 15 for determination of sulfadiazine is also available in the literature. However, the kinetic study of the drug is still lacking in the literature.…”

Differential pulse voltammetric determination and catalytic oxidation of sulfamethoxazole using [5,10,15,20‐ tetrakis (3‐methoxy‐4‐hydroxy phenyl) porphyrinato] Cu (II) modified carbon paste sensor

“…It is also used in veterinary practices. At present, the standard methods for the determination of SM are the spectrophotometric method, [1,2] the flow injection spectrophotometric method, [3] the Bratton-Marshall method, [3 -5] the titrimetric assay method, [6,7] gas chromatography and gas chromatography-mass spectrometry, [8] * capillary electrophoresis, [5] high performance liquid chromatography, [4] high performance thin layer chromatography, [9] liquid chromatography-mass spectrometry, [10] and voltammetry. [11] Among these, electrochemical detection is one of the promising approaches.…”