In this research, the effect of utilizing semi-attached rib on heat transfer and liquid turbulent flow of nanofluid watercopper oxide in three-dimensional rectangular microchannel has been investigated. The results of numerical examination of this study in comparison to those of smooth channel have also been evaluated. The range of Reynolds numbers is between 10,000 and 60,000 and the volume fraction of copper oxide nanoparticle in 0%, 2%, and 4% was examined. In this numerical simulation, the effects of the changes in parameters such as dimensions of semi-attached rib, volume fraction of the nanoparticle, and Reynolds number were considered. The results of this study showed that utilizing semiattached rib in microchannel with a ratio of 0 \ R/W 0.325 in producing stronger vortices, which causes better mixture in fluid layers, is weaker than that with tooth mode of R/W = 0 ratio. However, the main advantage of using tooth with a ratio of 0 \ R/W 0.325 in comparison to ordinary tooth is the increase in heat transfer and reduction in coefficient friction and pumping power.