Three-dimensional unsteady numerical simulations were conducted to investigate the detailed film cooling mechanism of the high-pressure turbine shroud with the first-stage turbine blade and guide vane for an aero-engine under the high-speed rotation of blades and rotor-stator interaction. The slip mesh was used to realize the relative motion between the rotating blade and the stationary turbine shroud. It is found that the coolant jet is alternately influenced by the hot mainstream, tip clearance leakage flow and leakage vortex due to the high rotational speed of blades. The film cooling characteristics of the turbine shroud significantly present an unsteady and periodic flow and heat transfer phenomenon. The insufficient cooling margin for film holes at the upstream of the blade leading edge can occur not only under high blowing ratios due to the coolant jet liftoff, but also at low blowing ratios due to the insufficient coolant flow rate as a result of the high exit pressure. A novel shroud cooling structure with coolant supply by the added throttle chamber is put forward, and expected to provide better thermal protection for the high-pressure turbine shroud near the leading edge of blades with no extra increase in the total mass flow rate of coolant.