Flowers of the Himalaya

Mathew, Brian; Polunin, Oleg; Stainton, Adam

doi:10.2307/4103012

Cited by 5 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The collected plants were dried and mounted on herbarium sheets. The identification of plant specimens was performed by a team of botanists headed by S. Rajbhandary, using relevant literature [ 17 , 18 , 19 , 20 ]. A voucher specimen was deposited at the Tribhuvan University Central Herbarium (TUCH), Kathmandu, Nepal, under the representative code: EK0420.…”

Section: Methodsmentioning

confidence: 99%

An Integrated LC-ESI-MSn and High Resolution LC-ESI-QTOF Approach for the Identification of Phloroglucinols from Nepalese Hypericum japonicum

et al. 2020

View full text Add to dashboard Cite

Phloroglucinols are characteristic constituents of Hypericumjaponicum that are claimed to exert several bioactivities, such as anti-inflammatory, anti-depressant and anti-viral ones. Phloroglucinols are unstable compounds and their synthesis is challenging; thus, isolation from natural sources is still one of the main strategies for obtaining these constituents in purified form. Assessing the presence of phloroglucinols in plant materials can be of interest for compound isolation, and LC-MS approaches afford sensitivity and specificity in this regard. In this work, we combined data from quadrupole-time of flight (QTOF) and ion trap (IT) mass spectrometers in order to assess the presence of the phloroglucinols characteristic of H. japonicum and to elucidate their MS fragmentation pathways. The identified compounds present similar structures bearing the 1,3,5-trihydroxybenzene core with different substitutions, which, in constituents at higher MW, is linked to 3′,3′-dimethyl-6′-oxo-phlorisobutyrophenone by a methylene bridge. Differences in MS2 spectra of the considered phloroglucinols are useful for compound identification and differentiation, and to perform dereplication studies. Overall, the proposed approach could be useful for the analysis of phloroglucinols in H. japonicum and other plant species.

show abstract