“…Sampling blood and measuring BCS and rumen size were conducted for 10 randomly selected cows at the end of FN period (on day 18) and for the same animals at the end of FW period (on day 48) in the same manner. Blood was drawn from the jugular vein about 4 hrs after the morning feeding (Okada, Sato, Sasaki, et al, 1997a;Watanabe, Kumagae, Noguchi, Maeta, & Konishi, 2015). Serum was separated within 15 min after collection and stored in a deep freezer until analysis (Okada & Yasuda, 2001).…”