Fluctuation of “sperm DNA integrity” in accordance with semen parameters, and it’s relationship with infertility

Durmaz, Ayşen; Dikmen, Nurten; Gündüz, Cumhur; Göker, Ege Nazan Tavmergen; Tavmergen, Erol

doi:10.1007/s10815-014-0364-3

Cited by 4 publications

(2 citation statements)

References 29 publications

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“…Sperm DNA fragmentation is depicted in the Figure and was observed to be significantly elevated in all of the infertile groups as compared to the fertile group, indicating that subjects that had low semen quality also had higher levels of DNA damage. Sperm DNA damage was significantly increased in infertile patients suffering from varicocele (n = 32) as compared to the fertile ones and could have been due to increased exposure to reactive oxygen species (46)(47)(48)(49). The sperm DNA damage in the infertile normozoospermic group with normal sperm parameters and the oligozoospermic group was significantly higher than that for the fertile subjects but not significantly different from that of the other infertile subgroups.…”

Section: Discussionmentioning

confidence: 83%

Human semen quality and sperm DNA damage assessed bycomet assay in clinical groups

Ramzan¹,

Khan²,

Ramzan³

et al. 2015

Turk J Med Sci

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Background/aim: About 10%-15% of couples around the world suffer from infertility. Male infertility is responsible directly or indirectly in ~60% of cases. A deficiency in semen is the most common cause of male infertility. Materials and methods:The study included 180 male subjects aged 18-50 years with 26 fertile and 154 infertile. The infertile subjects were further subdivided according to the WHO guidelines of semen analysis (2010) into different clinical groups. Sperm DNA damage was estimated using a neutral comet assay. Plasma gonadotropin and testosterone levels were measured using a chemiluminescence assay. Results:The results of the study revealed no significant differences in semen volume, pH, and liquefaction time between the fertile and all infertile groups. However, sperm concentration, sperm vitality, and sperm motility were significantly lower in all infertile groups as compared to the fertile males. The morphological forms of the sperm and its DNA fragmentation varied significantly between the fertile and infertile males. Reproductive hormone levels were observed to be significantly lower in the infertile than in the fertile males.Conclusion: Sperm DNA fragmentation was higher in all of the infertile subjects as compared to the fertile ones. Reproductive hormone levels varied significantly between the infertile patients and the fertile ones.

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Section: Discussionmentioning

confidence: 83%

Human semen quality and sperm DNA damage assessed bycomet assay in clinical groups

Ramzan¹,

Khan²,

Ramzan³

et al. 2015

Turk J Med Sci

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“…men [102]. Especially in idiopathic infertility, about 20% of patients have high levels of SDF, which can affect fertilization, embryo implantation, and survival [103].…”

Section: Effects Of Sdf On Male Infertilitymentioning

confidence: 99%

Progress in Research on Sperm DNA Fragmentation

Qiu

et al. 2020

Med Sci Monit

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With the increasing incidence of male infertility, routine detection of semen is insufficient to accurately assess male fertility. Infertile men, who have lower odds of conceiving naturally, exhibit high levels of sperm DNA fragmentation (SDF). The mechanisms driving SDF include abnormal spermatogenesis, oxidative stress damage, and abnormal sperm apoptosis. As these factors can induce SDF and subsequent radical changes leading to male infertility, detection of the extent of SDF has become an efficient routine method for semen analysis. Although it is still debated, SDF detection has become a research hotspot in the field of reproductive medicine as a more accurate indicator for assessing sperm quality and male fertility. SDF may be involved in male infertility, reproductive assisted outcomes, and growth and development of offspring. The effective detection methods of SDF are sperm chromatin structure analysis (SCSA), terminal transferase-mediated dUTP end labeling (TUNEL) assay, single-cell gel electrophoresis (SCGE) assay, and sperm chromatin dispersion (SCD) test, and all of these methods are valuable for assisted reproductive techniques. Currently, the preferred method for detecting sperm DNA integrity is SCSA. However, the regulation network of SDF is very complex because the sperm DNA differs from the somatic cell DNA with its unique structure. A multitude of molecular factors, including coding genes, non-coding genes, or methylated DNA, participate in the complex physiological regulation activities associated with SDF. Studying SDF occurrence and the underlying mechanisms may effectively improve its clinical treatments. This review aimed to outline the research status of SDF mechanism and detection technology-related issues, as well as the effect of increased SDF rate, aiming to provide a basis for clinical male infertility diagnosis and treatment.

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Clinical Utility of Sperm Function Tests in Predicting Male Fertility: A Systematic Review

Sanyal,

Arya,

Nishi

et al. 2023

Reprod. Sci.

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Fluctuation of “sperm DNA integrity” in accordance with semen parameters, and it’s relationship with infertility

Cited by 4 publications

References 29 publications

Human semen quality and sperm DNA damage assessed bycomet assay in clinical groups

Human semen quality and sperm DNA damage assessed bycomet assay in clinical groups

Progress in Research on Sperm DNA Fragmentation

Clinical Utility of Sperm Function Tests in Predicting Male Fertility: A Systematic Review

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