We study the behavior of the stationary velocity of a driven particle in an environment of mobile hard-core obstacles. Based on a lattice gas model, we demonstrate analytically that the drift velocity can exhibit a nonmonotonic dependence on the applied force, and show quantitatively that such negative differential mobility (NDM), observed in various physical contexts, is controlled by both the density and diffusion time scale of obstacles. Our study unifies recent numerical and analytical results obtained in specific regimes, and makes it possible to determine analytically the region of the full parameter space where NDM occurs. These results suggest that NDM could be a generic feature of biased (or active) transport in crowded environments.PACS numbers: 05.40.Fb,83.10.Pp Introduction.-Quantifying the response of a complex system to an external force is one of the cornerstone problems of statistical mechanics. In the linear response regime, a fundamental result is the fluctuationdissipation theorem, which relates system response and spontaneous fluctuations. Within the last years a great effort has been devoted to generalizations of this theorem to nonequilibrium situations [1][2][3][4], when the time reversal symmetry is broken, and also to elucidating the effects of the higher order contributions in the external perturbation [5][6][7][8][9][10][11]. From experimental perspective, theoretical understanding of the latter issues is of an utmost importance in several fields, such as active microrheology [12][13][14] and dynamics of nonequilibrium fluids [15,16].A striking example of anomalous behavior beyond the linear regime is the negative response of a particle's velocity to an applied force, observed in diverse situations in which a particle subject to an external force F travels through a medium. The terminal drift velocity V (F ) attained by the driven particle is then a nonmonotonic function of the force: upon a gradual increase of F , the terminal drift velocity first grows as expected from linear response, reaches a peak value and eventually decreases. This means that the differential mobility of the driven particle becomes negative for F exceeding a certain threshold value. Such a counter-intuitive "getting more from pushing less" [17] behavior of the differential mobility (or of the differential conductivity) has been observed for a variety of physical systems and processes, e.g. for electron transfer in semiconductors at low temperatures [18][19][20][21], hopping processes in disordered media [22], transport of electrons in mixtures of atomic gases with reactive collisions [23], far-from-equilibrium quantum spin chains [24], some models of Brownian motors [25,26], soft matter colloidal particles [27], different nonequilibrium systems [17], and also for the kinetically constrained models of glass formers [28][29][30].Apart of these examples, negative differential mobility (NDM) has been observed in the minimal model of a driven lattice gas, which captures many essential features of the behavior in r...