Fluid dynamics in zebrafish Kupffer's vesicle

Okabe, Noriko; Xu, Bo; Burdine, Rebecca D.

doi:10.1002/dvdy.21730

Cited by 72 publications

(63 citation statements)

References 45 publications

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“…The KV is a transient fluid filled organ derived from the dorsal forerunner cells that plays a critical role in establishing L/R polarity (Cooper and D’Amico, 1996; Essner et al, 2005; Kramer-Zucker et al, 2005; Melby et al, 1996; Okabe et al, 2008). We have generated a transgenic reporter line Tg(dusp6:d2EGFP) pt6 that expresses GFP in response to FGF signaling (Molina et al, 2007).…”

Section: Resultsmentioning

confidence: 99%

Overlapping functions of Pea3 ETS transcription factors in FGF signaling during zebrafish development

Znosko

Thomas

et al. 2010

Developmental Biology

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Fibroblast Growth Factors (FGFs) are secreted molecules that activate the RAS/mitogen-activated protein kinase (MAPK) signaling pathway. In zebrafish development, FGF signaling is responsible for establishing dorsal polarity, maintaining the isthmic organizer, and cardiac ventricle formation. Because several ETS factors are known transcriptional mediators of MAPK signaling, we hypothesized that these factors function to mediate FGF signaling processes. In zebrafish, the simultaneous knock-down of three Pea3 ETS proteins, Etv5, Erm, and Pea3, produced phenotypes reminiscent of embryos deficient in FGF signaling. Morphant embryos displayed both cardiac and left/right patterning defects as well as disruption of the isthmic organizer. Furthermore, the expression of FGF target genes was abolished in Pea3 ETS depleted embryos. To understand how FGF signaling and ETS factors control gene expression, transcriptional regulation of dusp6 was studied in mouse and zebrafish. Conserved Pea3 ETS binding sites were identified within the Dusp6 promoter, and reporter assays showed that one of these sites is required for dusp6 induction by FGFs. We further demonstrated the interaction of Pea3 ETS factors with the Dusp6 promoter both in vitro and in vivo. These results revealed the requirement of ETS factors in transducing FGF signals in developmental processes.

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Section: Resultsmentioning

confidence: 99%

Overlapping functions of Pea3 ETS transcription factors in FGF signaling during zebrafish development

Znosko

Thomas

et al. 2010

Developmental Biology

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“…In zebrafish, the cellular architecture of KV is asymmetric along the anteroposterior and dorsoventral axes. KV cells in the dorsoanterior region develop an elongated columnar morphology with small apical surfaces-which allows tight packing of these cells-whereas ventroposterior cells adopt a cuboidal morphology with large apical surfaces [37,38]. This process, referred to as 'KV remodelling' [72], places more motile cilia in the dorsoanterior region to drive strong leftward flow across the anterior pole as described above.…”

Section: (B) Positioning Of Ciliated Cellsmentioning

confidence: 95%

Cilia in vertebrate left–right patterning

Dasgupta

Amack

2016

Phil. Trans. R. Soc. B

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One contribution of 17 to a theme issue 'Provocative questions in left -right asymmetry'. Understanding how left-right (LR) asymmetry is generated in vertebrate embryos is an important problem in developmental biology. In humans, a failure to align the left and right sides of cardiovascular and/or gastrointestinal systems often results in birth defects. Evidence from patients and animal models has implicated cilia in the process of left-right patterning. Here, we review the proposed functions for cilia in establishing LR asymmetry, which include creating transient leftward fluid flows in an embryonic 'left -right organizer'. These flows direct asymmetric activation of a conserved Nodal (TGFb) signalling pathway that guides asymmetric morphogenesis of developing organs. We discuss the leading hypotheses for how cilia-generated asymmetric fluid flows are translated into asymmetric molecular signals. We also discuss emerging mechanisms that control the subcellular positioning of cilia and the cellular architecture of the left-right organizer, both of which are critical for effective cilia function during left-right patterning. Finally, using mosaic cell-labelling and timelapse imaging in the zebrafish embryo, we provide new evidence that precursor cells maintain their relative positions as they give rise to the ciliated left -right organizer. This suggests the possibility that these cells acquire left-right positional information prior to the appearance of cilia.This article is part of the themed issue 'Provocative questions in leftright asymmetry'.

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“…In addition, Ca 2+ oscillation in the cilia is thought to regulate left-right asymmetry 42)43)44). Although the cilia of the KV are motile,20) they are proposed to express PKD2 and sense extracellular Ca 2+ , as the immotile cilia in mouse perinodal crown cells do, although the cilia have 9+2 type microtubules in the KV 4). The movement of cilia and intracellular Ca 2+ can be monitored by the small GTPase, Arl13b, tagged with genetically encoded Ca 2+ indicators, GCaMP5 or RGECO1 42).…”

Section: Kupffer's Vesicle For Determination Of Left-right Asymmetrymentioning

confidence: 99%

“…The dorsal forerunner cells (DFCs) of the endoderm give rise to KV-forming cells 18)19). Subsequently, these cells become ciliated to promote unidirectional flow in the KV 20). Motile cilia rotate unidirectionally from right to left to induce unidirectional flow in the KV 21).…”

Section: Introductionmentioning

confidence: 99%

Spatial Allocation and Specification of Cardiomyocytes during Zebrafish Embryogenesis

et al. 2017

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Incomplete development and severe malformation of the heart result in miscarriage of embryos because of its malfunction as a pump for circulation. During cardiogenesis, development of the heart is precisely coordinated by the genetically-primed program that is revealed by the sequential expression of transcription factors. It is important to investigate how spatial allocation of the heart containing cardiomyocytes and other mesoderm-derived cells is determined. In addition, the molecular mechanism underlying cardiomyocyte differentiation still remains elusive. The location of ectoderm-, mesoderm-, and endoderm-derived organs is determined by their initial allocation and subsequent mutual cell-cell interactions or paracrine-based regulation. In the present work, we provide an overview of cardiac development controlled by the germ layers and discuss the points that should be uncovered in future for understanding cardiogenesis.

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Fluid dynamics in zebrafish Kupffer's vesicle

Abstract: Work in mouse has implicated cilia motility and leftward nodal flow as the mechanism for breaking left-right symmetry. In zebrafish, it is assumed that Kupffer's vesicle is analogous to the mouse node.

Cited by 72 publications

References 45 publications

Overlapping functions of Pea3 ETS transcription factors in FGF signaling during zebrafish development

Overlapping functions of Pea3 ETS transcription factors in FGF signaling during zebrafish development

Cilia in vertebrate left–right patterning

Spatial Allocation and Specification of Cardiomyocytes during Zebrafish Embryogenesis

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