Fluid resuscitation with hydroxyethyl starch in perioperative acute hemorrhagic shock

Hirata, Naoyuki

doi:10.1007/s00540-020-02748-0

Cited by 4 publications

(2 citation statements)

References 31 publications

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“…HES shows a more rapid curative effect compared with LR and can better maintain blood pressure and provide adequate tissue oxygenation and peripheral perfusion in fluid resuscitation [ 21 , 22 ]. Moreover, the nephrotoxic effect of HES [ 20 ] mainly manifests in patients with septic shock rather than those with hemorrhagic shock [ 23 ]. On the other hand, Li et al [ 24 ] demonstrated that under inflammatory conditions, HES has an anti-inflammatory function through reducing neutrophil activation, platelet-neutrophil aggregation, and extraneutrophil trap.…”

Section: Discussionmentioning

confidence: 99%

Hydroxyethyl Starch Improves the Prognosis of Rats with Traumatic Shock via Activation of the ERK Signaling Pathway in Lymphocytes

Liu¹,

Lu²,

Dong³

et al. 2022

Computational and Mathematical Methods in Medicine

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Objective. Severe traumatic shock is one of the leading causes of death in young adults. A large number of studies have shown that effective volumetry resuscitation on the basis of controlled injury can not only increase the success rate of early resuscitation but also reduce systemic inflammatory response and improve the cure rate of severe traumatic shock. The study explored the effects of hydroxyethyl starch (HES) on the survival rate, lymphocyte function and proliferation of rats with traumatic shock, and the potential mechanisms. Methods. Traumatic shock was constructed in rats as experimental model, and liquid resuscitation was performed using HES and lactated Ringer’s (LR). 24-h mortality was recorded, and lymphocytes were isolated. The expressions of signaling pathway factors was detected by qPCR and Western blot. ELISA was performed to determine the expression of interleukin 6 (IL-6) and tumor necrosis factor-α (TNF-α) in cell supernatant. Results. HES for fluid resuscitation augmented the survival of traumatic shock rats, upregulated the expressions of MEK and ERK1/2, and downregulated the expressions of IL-6 and TNF-α. However, inhibition of ERK signaling pathway reversed the effect of HES on the immune improvement and the 24-h survival rate of the traumatic shock rats ( P < 0.05 ). Conclusion. HES could exert the anti-inflammatory effects on lymphocytes by mediating the phosphorylation of proteins of the ERK signaling pathway. HSE demonstrated a high efficacy in effectively treating traumatic shock, thus could be used in clinical practice.

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Section: Discussionmentioning

confidence: 99%

Hydroxyethyl Starch Improves the Prognosis of Rats with Traumatic Shock via Activation of the ERK Signaling Pathway in Lymphocytes

Liu¹,

Lu²,

Dong³

et al. 2022

Computational and Mathematical Methods in Medicine

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“…HES can be used in the rescue of hemorrhagic shock, general anesthesia, drug carrier, etc. Many studies have shown that 6% HES 130/0.4 has certain anti-inflammatory effect 13,14,15) , but there are few studies on the anti-inflammatory effect of HES 200/0.5 at present.…”

Section: Introductionmentioning

confidence: 99%

The Anti-Inflammatory Effect of 6% HES 200/0.5 on RAW264.7 Cells Induced by LPS through HMGB1/NF-κB Signaling Pathway

Zhang

Wang

Zhang

et al. 2022

J. Hard Tissue Biology.

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We investigated the anti-inflammatory effect of hydroxyethyl starch (HES) 6% 200/0.5 on lipopolysaccharide (LPS)-induced RAW264.7 cells based on the high mobility group protein B1/nuclear transcription factor-κB (HMGB1/NF-κB) signaling pathway. The mouse monocyte macrophage RAW264.7 cells were divided into control group (no additional treatment), LPS group (0.2 pg/ml LPS), LPS+HES group (0.2 pg/ml LPS+6% HES) and LPS+TAK-242 (HMGB1/NF-κB signal pathway blocker) group (0.2 pg/ml LPS The LPS+HES+TAK-242 group (0.2 pg/ml LPS+6% HES+1 μ mol/l TAK-242) was cultured for 48 hours. Griess method and ELISA were used to detect the levels of nitric oxide (NO), tumor necrosis factor α (TNF-α), interleukin (IL)-6 and IL-1β in cell supernatant. The level of intracellular reactive oxygen species (ROS) was detected by flow cytometry. The mitochondrial membrane potential was detected by immunofluorescence. The mRNA expressions of inducible nitric oxide synthase (iNOS), HMGB1, Toll-like receptor 4 (TLR4), nuclear factor-κ b inhibitory protein α (IκBα) and NF-κB were detected by qRT-PCR. The expressions of HMGB1, TLR4, IκBα, phosphorylated IκBα (p-IκBα), NF-κB and phosphorylated NF-κB (p-NF-κB) were detected by western blotting. Compared with the control group, the expression levels of NO, TNF-ɑ, IL-6, IL-1β, ROS, iNOS, HMGB1, TLR4, NF-κB mRNA, HMGB1, TLR4 and p-NF-κB proteins in the LPS group, LPS+HES group, LPS+TAK-242 group and LPS+HES+TAK-242 group increased (P<0.05), the expression levels of mitochondrial membrane potential, IκBα mRNA and p-IκBα protein decreased (P<0.05). Compared with LPS group, the expression levels of NO, TNF-ɑ, IL-6, IL-1β, ROS, iNOS, HMGB1, TLR4, NF-κB mRNA, HMGB1, TLR4 and p-NF-κB proteins in the LPS+HES group, LPS+TAK-242 group and LPS+HES+TAK-242 group increased (P<0.05), the expression levels of mitochondrial membrane potential, IκBα mRNA and p-IκBα protein decreased (P<0.05). The expression levels of mitochondrial membrane potential, IκBα mRNA and p-IκBα protein increased (P<0.05). Six % HES 200/0.5 could down-regulate the expression of molecules related to HMGB1/NF-кB signaling pathway, and reduce the inflammatory response of RAW264.7 cells induced by LPS.

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Assessing fluid responsiveness during spontaneous breathing

Suehiro¹

2022

J Anesth

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Fluid resuscitation with hydroxyethyl starch in perioperative acute hemorrhagic shock

Cited by 4 publications

References 31 publications

Hydroxyethyl Starch Improves the Prognosis of Rats with Traumatic Shock via Activation of the ERK Signaling Pathway in Lymphocytes

Hydroxyethyl Starch Improves the Prognosis of Rats with Traumatic Shock via Activation of the ERK Signaling Pathway in Lymphocytes

The Anti-Inflammatory Effect of 6% HES 200/0.5 on RAW264.7 Cells Induced by LPS through HMGB1/NF-κB Signaling Pathway

Assessing fluid responsiveness during spontaneous breathing

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