Fluid shear stress suppresses ICAM-1-mediated transendothelial migration of leukocytes in coculture model

Sakamoto, Naoya; Ueki, Yosuke; Oi, Masaki; Kiuchi, Takuya; Sato, Masaaki

doi:10.1016/j.bbrc.2018.05.182

Cited by 10 publications

(7 citation statements)

References 28 publications

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“…Some studies reported the reduction of adhesion molecules expression in sheared EC, as ICAM-1 and CTGF [ 9 , 66 ]. In vivo, atheroprotected sites of the endothelium also exhibit lower expressions of adherens junction proteins.…”

Section: Resultsmentioning

confidence: 99%

Exposure of human cerebral microvascular endothelial cells hCMEC/D3 to laminar shear stress induces vascular protective responses

Choublier

Taghi

Menet

et al. 2022

Fluids Barriers CNS

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Endothelial cells (ECs) are constantly submitted in vivo to hemodynamical forces derived from the blood circulation, including shear stress (SS). ECs are able to detect SS and consequently adapt their phenotype, thus affecting many endothelial functions. If a plethora of shear stress-regulated molecular networks have been described in peripheral ECs, less is known about the molecular responses of microvascular brain ECs which constitute the blood–brain barrier (BBB). In this work, we investigated the response of human cerebral microvascular ECs to laminar physiological shear stress using the well characterized hCMEC/D3 cell line. Interestingly, we showed that hCMEC/D3 cells responded to shear stress by aligning perpendicularly to the flow direction, contrary to peripheral endothelial cells which aligned in the flow direction. Whole proteomic profiles were compared between hCMEC/D3 cells cultured either in static condition or under 5 or 10 dyn.cm−2 SS for 3 days. 3592 proteins were identified and expression levels were significantly affected for 3% of them upon both SS conditions. Pathway analyses were performed which revealed that most proteins overexpressed by SS refer to the antioxidant defense, probably mediated by activation of the NRF2 transcriptional factor. Regarding down-regulated proteins, most of them participate to the pro-inflammatory response, cell motility and proliferation. These findings confirm the induction of EC quiescence by laminar physiological SS and reveal a strong protective effect of SS on hCMEC/D3 cells, suggesting a similar effect on the BBB. Our results also showed that SS did not significantly increase expression levels nor did it affect the localization of junctional proteins and did not afect either the functional activity of several ABC transporters (P-glycoprotein and MRPs). This work provides new insights on the response of microvascular brain ECs to SS and on the importance of SS for optimizing in vitro BBB models.

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Section: Resultsmentioning

confidence: 99%

Exposure of human cerebral microvascular endothelial cells hCMEC/D3 to laminar shear stress induces vascular protective responses

Choublier

Taghi

Menet

et al. 2022

Fluids Barriers CNS

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“…Besides the well‐known risk factors such as smoking, hypercholesterolemia, hypertension, and diabetes, shear stress also plays an important role in the onset and development of AS (da Silva et al, 2019). The shear stress can induce dramatic changes in signaling and gene expression in endothelial cells (ECs), which in turn, modulate cell death, migration, growth, and proliferation (Sakamoto, Ueki, Oi, Kiuchi, & Sato, 2018). The value of physiological shear stress ranges from 5 to 12 dynes/cm 2 , while a value <5 dynes/cm 2 is considered to be low shear stress and thought to be pro‐AS shear stress (Zarins et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

Low shear stress regulates vascular endothelial cell pyroptosis through miR‐181b‐5p/STAT‐3 axis

Yang

Wang

et al. 2020

Journal Cellular Physiology

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“…Coculturing with SMCs increases the expression of adhesion proteins such as intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selection on the surface of ECs, as well as the expression and secretion of chemokines and cytokines such as monocyte chemotactic protein-1 (MCP-1), growth-related oncogene-α (GRO-α), and interleukin-8 (IL-8) that promote leukocyte migration, and these expressions and secretions induced by the coculture are suppressed by a physiological level of 1.5 Pa WSS condition [ 62 , 63 , 69 , 82 ]. It has also been shown that WSS applied to ECs suppressed leukocyte invasion stimulated by an SMC coculture environment [ 83 ]. The WSS conditions of ECs also affect the phenotype of cocultured SMCs.…”

Section: Resultsmentioning

confidence: 99%

A Review of Functional Analysis of Endothelial Cells in Flow Chambers

Ohta

Sakamoto

Funamoto

et al. 2022

JFB

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The vascular endothelial cells constitute the innermost layer. The cells are exposed to mechanical stress by the flow, causing them to express their functions. To elucidate the functions, methods involving seeding endothelial cells as a layer in a chamber were studied. The chambers are known as parallel plate, T-chamber, step, cone plate, and stretch. The stimulated functions or signals from endothelial cells by flows are extensively connected to other outer layers of arteries or organs. The coculture layer was developed in a chamber to investigate the interaction between smooth muscle cells in the middle layer of the blood vessel wall in vascular physiology and pathology. Additionally, the microfabrication technology used to create a chamber for a microfluidic device involves both mechanical and chemical stimulation of cells to show their dynamics in in vivo microenvironments. The purpose of this study is to summarize the blood flow (flow inducing) for the functions connecting to endothelial cells and blood vessels, and to find directions for future chamber and device developments for further understanding and application of vascular functions. The relationship between chamber design flow, cell layers, and microfluidics was studied.

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Fluid shear stress suppresses ICAM-1-mediated transendothelial migration of leukocytes in coculture model

Cited by 10 publications

References 28 publications

Exposure of human cerebral microvascular endothelial cells hCMEC/D3 to laminar shear stress induces vascular protective responses

Exposure of human cerebral microvascular endothelial cells hCMEC/D3 to laminar shear stress induces vascular protective responses

Low shear stress regulates vascular endothelial cell pyroptosis through miR‐181b‐5p/STAT‐3 axis

A Review of Functional Analysis of Endothelial Cells in Flow Chambers

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