Fluidity of intact erythrocyte membranes. Correction for fluorescence energy transfer from diphenylhexatriene to hemoglobin

Plášek, Jaromı́r; Čermáková, Dana; Jarolím, Petr

doi:10.1016/0005-2736(88)90171-x

Cited by 10 publications

(4 citation statements)

References 20 publications

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“…Assuming that the preferred distribution of fluorescent DPH molecules is normal to the membrane surface, the dye molecules can detect the movement of the ends of the phospholipid chain in the region of the membrane core [ 48 , 49 ]. Thus, the enhanced disorder associated with the multiple double bonds in DHA is the likely molecular origin for the observed lower anisotropy of the fluorescent probe DPH at the 6 and 12-week supplementation in both groups.…”

Section: Discussionmentioning

confidence: 99%

Lipid Profile, Lipoprotein Subfractions, and Fluidity of Membranes in Children and Adolescents with Depressive Disorder: Effect of Omega-3 Fatty Acids in a Double-Blind Randomized Controlled Study

et al. 2020

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Depressive disorder (DD) is a psychiatric disorder whose molecular basis is not fully understood. It is assumed that reduced consumption of fish and omega-3 fatty acids (FA) is associated with DD. Other lipids such as total cholesterol (TCH), LDL-, and HDL-cholesterols (LDL-CH, HDL-CH) also play a role in depression. The primary endpoint of the study was the effect of omega-3 FA on the severity of depression in children and adolescents. This study aimed to investigate the secondary endpoint, relationship between depressive disorder symptoms and lipid profile, LDL- and HDL-cholesterol subfractions, Paraoxonase 1 (PON1) activities, and erythrocyte membrane fluidity in 58 depressed children and adolescents (calculated by the statistical program on the effect size), as well as the effect of omega-3 FA on the monitored parameters. Depressive symptoms were assessed by the Children’s Depression Inventory (CDI), lipid profile by standard biochemical procedures, and LDL- and HDL-subfractions by the Lipoprint system. Basic biochemical parameters including lipid profile were compared with levels in 20 healthy children and were in the physiological range. Improvement of symptoms in the group supplemented with a fish oil emulsion rich in omega-3 FA in contrast to omega-6 FA (emulsion of sunflower oil) has been observed. We are the first to report that omega-3 FAs, but not omega-6 FA, increase large HDL subfractions (anti-atherogenic) after 12 weeks of supplementation and decrease small HDL subfractions (proatherogenic) in depressed children. We found a negative correlation between CDI score and HDL-CH and the large HDL subfraction, but not LDL-CH subfractions. CDI score was not associated with erythrocyte membrane fluidity. Our results suggest that HDL-CH and its subfractions, but not LDL-CH may play a role in the pathophysiology of depressive disorder. The study was registered under ISRCTN81655012.

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Section: Discussionmentioning

confidence: 99%

Lipid Profile, Lipoprotein Subfractions, and Fluidity of Membranes in Children and Adolescents with Depressive Disorder: Effect of Omega-3 Fatty Acids in a Double-Blind Randomized Controlled Study

et al. 2020

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“…Some researchers have performed investigations on the mechanical and dynamic behavior of hemoglobin from a molecular standpoint (Arroyo-Mañez et al, 2011; Kakar, Hoffman, Storz, Fabian, & Hargrove, 2010; Koshiyama & Wada, 2011; Xu, Tobi, & Bahar, 2003), and some studies that have been focused on the molecular mechanical behavior of hemoglobin variants such as sickled hemoglobin (HbS) (Li, Ha, & Lykotrafitis, 2012; Li & Lykotrafitis, 2011) and glycated hemoglobin (De Rosa et al, 1998). There have also been studies that have addressed the anisotropic behavior of hemoglobin structures using fluorescence based methods (Bucci & Steiner, 1988; Chaudhuri, Chakraborty, & Sengupta, 2011; Hegde, Sandhya, & Seetharamappa, 2013; Kantar, Giorgi, Curatola, & Fiorini, 1992; Plášek, Čermáková, & Jarolím, 1988) but did not address the molecular mechanical behavior. In sum, these previous investigations were limited since they did not explore the molecular mechanical behavior of hemoglobin (or its variants) under mechanical compression or shear loading conditions.…”

Section: Introductionmentioning

confidence: 99%

Molecular dynamics simulations indicate that deoxyhemoglobin, oxyhemoglobin, carboxyhemoglobin, and glycated hemoglobin under compression and shear exhibit an anisotropic mechanical behavior

Yesudasan

Wang

Averett

2017

Journal of Biomolecular Structure and Dynamics

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We developed a new mechanical model for determining the compression and shear mechanical behavior of four different hemoglobin structures. Previous studies on hemoglobin structures have focused primarily on overall mechanical behavior; however, this study investigates the mechanical behavior of hemoglobin, a major constituent of red blood cells (RBCs), using steered molecular dynamics (SMD) simulations to obtain anisotropic mechanical behavior under compression and shear loading conditions. Four different configurations of hemoglobin molecules were considered: deoxyhemoglobin (deoxyHb), oxyhemoglobin (HbO2), carboxyhemoglobin (HbCO), and glycated hemoglobin (HbA1C). The SMD simulations were performed on the hemoglobin variants to estimate their unidirectional stiffness and shear stiffness. Although hemoglobin is structurally denoted as a globular protein due to its spherical shape and secondary structure, our simulation results show a significant variation in the mechanical strength in different directions (anisotropy) and also a strength variation among the four different hemoglobin configurations studied. The glycated hemoglobin molecule possesses an overall higher compressive mechanical stiffness and shear stiffness when compared to deoxyhemoglobin, oxyhemoglobin, and carboxyhemoglobin molecules. Further results from the models indicate that the hemoglobin structures studied possess a soft outer shell and a stiff core based on stiffness.

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“…Assuming, that the preferred distribution of fluorescent DPH molecules is along the normal to the membran e surface, the dye molecules can detect the movement of phospholipid chain ends in the core region of the membrane [49,50]. So the enhanced disorder associated with the multiple double bonds in DHA is the likely molecular origin for the observed lower anisotropy of the fluorescent probe DPH in the 6 and 12-weeks´ supplementation in both groups indicate that the membranes had "looser" membrane structure in comparison to their respective baseline values.…”

Section: Discussionmentioning

confidence: 99%

Lipid Profile, Lipoprotein Subfractions, and Fluidity of Membranes in Children and Adolescents with Depressive Disorder: Effect of Omega-3 Fatty Acids in A Double-blind Randomized Controlled Study

Katrenčíková

Vaváková

Waczulı́ková

et al. 2020

Preprint

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Depressive disorder (DD) is a psychiatric disorder whose molecular basis is not fully understood. It is assumed that reduced consumption of fish and omega-3 fatty acids (FA) is associated with DD. Other lipids like total cholesterol (TCH), LDL- and HDL-cholesterols (LDL-CH, HDL-CH) also play a role in depression. This study aimed to investigate the relationship between depressive disorder symptoms and lipid profile, LDL- and HDL-cholesterol subfractions, Paraoxonase 1 (PON1) activities and erythrocyte membrane fluidity in 58 depressive children and adolescents, as well as the effect of omega-3 FA on the monitored parameters. Depressive symptoms were assessed by the Children's Depression Inventory (CDI), lipid profile by standard biochemical procedures, LDL- and HDL-subfractions by the Lipoprint system. Basic biochemical parameters including lipid profile were compared with levels in 20 healthy children and were in the physiological range. We are the first to report that omega-3 FAs increase after 12 weeks of supplementation large HDL subfractions (anti-atherogenic) and decrease small HDL subfractions (pro-atherogenic) in depressed children. We found a negative correlation between CDI score and HDL-CH and large HDL subfraction, but not LDL-CH subfractions. CDI score was not associated with erythrocyte membrane fluidity. Our results suggest that HDL-CH and its subfractions, but not LDL-CH may play a role in the pathophysiology of depressive disorder.

show abstract

Fluidity of intact erythrocyte membranes. Correction for fluorescence energy transfer from diphenylhexatriene to hemoglobin

Cited by 10 publications

References 20 publications

Lipid Profile, Lipoprotein Subfractions, and Fluidity of Membranes in Children and Adolescents with Depressive Disorder: Effect of Omega-3 Fatty Acids in a Double-Blind Randomized Controlled Study

Lipid Profile, Lipoprotein Subfractions, and Fluidity of Membranes in Children and Adolescents with Depressive Disorder: Effect of Omega-3 Fatty Acids in a Double-Blind Randomized Controlled Study

Molecular dynamics simulations indicate that deoxyhemoglobin, oxyhemoglobin, carboxyhemoglobin, and glycated hemoglobin under compression and shear exhibit an anisotropic mechanical behavior

Lipid Profile, Lipoprotein Subfractions, and Fluidity of Membranes in Children and Adolescents with Depressive Disorder: Effect of Omega-3 Fatty Acids in A Double-blind Randomized Controlled Study

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