Fluidity of LM cell membranes with modified lipid compositions as determined with 1,6-diphenyl-1,3,5-hexatriene

Gilmore, Reid; Cohn, Nina; Gläser, Michael

doi:10.1021/bi00573a017

Cited by 62 publications

(28 citation statements)

References 45 publications

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“…A change in phospholipid composition upon supplementation with stearic acid, oleic acid or linoleic acid has been described in neuroblastoma x glioma hybrid cells [29] and other cell lines [31,32].…”

Section: Introductionmentioning

confidence: 99%

Effect of fatty acids on plasma membrane lipid dynamics and cation permeability in neuroblastoma cells

Boonstra

Nelemans

Feijen

et al. 1982

Biochimica et Biophysica Acta (BBA) - Biomembranes

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In this study the effects of experimental modifications of plasma membrane lipid lateral mobility on the electrical membrane properties and cation transport of mouse neuroblastoma cells, clone Neuro-2A, have been studied. Short-term supplementation of a chemically defined growth medium with oleic acid or linoleic acid resulted in an increase in the lateral mobility of lipids as inferred from fluorescence recovery after photobleaching of the lipid probe 3,3'-dioctadecylindocarbocyanide iodide. These changes were accompanied by a marked depolarization of the membrane potential from -51 mV to -36 mV, 1.5 h after addition, followed by a slow repolarization. Tracer flux studies, using S6Rb÷ as a radioactive tracer for K ÷ , demonstrated that the depolarization was not caused by changes in (Na ÷ + K + )-ATPase-mediated K + influx or in the transmembrane K ÷ gradient. The permeability ratio (PNa/PK), determined from electrophysiological measurements, however, increased from 0.10 to 0.27 upon supplementation with oleic acid or linoleic acid. This transient rise of PN,/Px Was shown by 24Na+ and S6Rb+ flux measurements to be due to both an increase of the Na + permeability and a decrease of the K ÷ permeability. None of these effects occurred upon supplementation of the growth medium with stearic acid.

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Section: Introductionmentioning

confidence: 99%

Effect of fatty acids on plasma membrane lipid dynamics and cation permeability in neuroblastoma cells

Boonstra

Nelemans

Feijen

et al. 1982

Biochimica et Biophysica Acta (BBA) - Biomembranes

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“…Many of the studies on domains in biological membranes are interpreted in terms of the existence of gel-phase domains. However, the lipid compositions of most biological membranes indicate that gel-phase lipids are largely absent from membranes at the growth temperature (44,45). Lipids in domains of nonbilayer phases also have been postulated to occur in biological membranes (46), and additional evidence is needed to determine how extensive these phases are under physiological conditions.…”

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confidence: 99%

Characterization of lipid domains in erythrocyte membranes.

Rodgers

Gläser

1991

Proc. Natl. Acad. Sci. U.S.A.

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Fluorescence digital imaging microscopy was used to study the lateral distribution of the lipid components in erythrocyte membranes. Intact erythrocytes labeled with phospholipids containing a fluorophore attached to one fatty acid chain showed an uneven distribution ofthe phospholipids in the membrane thereby demonstrating the presence of membrane domains. The enrichment of the lipotropic compound chlorpromazine in domains in intact erythrocytes also suggested that the domains are lipid-enriched regions. Similar membrane domains were present in erythrocyte ghosts. The phospholipid enrichment was increased in the domains by inducing membrane protein aggregation. Double-labeling experiments were done to determine the relative distributions of different phospholipids in the membrane. Vesicles made from extracted lipids did not show the presence of domains consistent with the conclusion that membrane proteins were responsible for creating the domains. Overall, it was found that large domains exist in the red blood cell membrane with unequal enrichment of the different phospholipid species.Since it was proposed almost 20 years ago, the Fluid Mosaic Model has served as a foundation for understanding membrane structure and function (1). The model describes biological membranes as a mixture of lipid and protein components in a phospholipid bilayer where all the components can undergo free lateral motion. This results in a random distribution of the membrane components with no long-range order. One refinement to this model is the role of the cytoskeleton in anchoring transmembrane proteins (2). Another change is that biological membranes may be more heterogeneous than depicted by the Fluid Mosaic Model with the existence of relatively large domains with compositions different from the bulk membrane.

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“…Although the rotational relaxation and microviscosity of fluorescence probes that are nonspecifically located in the cellular plasma membrane have been examined, few studies have dealt directly with fluorescence probes that are specifically associated with a single defined membrane constituent. Experiments of this latter variety are of importance in view of recent data (14,15) that raise serious questions as to the validity of interpreting the dynamic properties of individual membrane proteins from studies using nonspecific fluorescence probes. The membrane is a highly heterogeneous environment, and localized interactions clearly play an important part in defining its dynamic properties.…”

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confidence: 99%

Cytoskeletal constraint of the beta-adrenergic receptor in frog erythrocyte membranes.

Cherksey¹,

Zadunaisky²,

Murphy³

1980

Proc. Natl. Acad. Sci. U.S.A.

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A fluorescence receptor binding assay, based upon the high-affinity fi-adrenergic receptor antagonist propranolol, is utilized to probe the microenvironment of the antagonist-receptor complex in the frog (Rana catesbeiana) erythrocyte membrane. The technique of steady-state fluorescence depolarization is applied to the propranolol-receptor complex, allowing quantitation of the rotational relaxation time of the complex. It is found that the complex is dynamically constrained at 20'C. However, in the temperature range 6-100C a sharp reversible release of constraint is observed. It is further demonstrated that the addition of drugs that are known to specifically disrupt the cytoskeleton (colchicine, vincristine, and vinblastine) causes a similar but irreversible release of constraint at 200C. Cytochalasin B has a much smaller influence on the rotational mobility of the propranolol-receptor complex than do the other drugs that disrupt the cytoskeleton. Amphotericin B is without effect on the rotational constraint of the complex. Binding of the antagonist 13H~dihydroalprenolol is not influenced by colchicine. A model is proposed which postulates that cytoskeletal elements are linked to the antagonist-receptor complex. Antagonist binding does not result in cytoskeleta release, whereas agonist binding is postulated to lead to dissociation of the agonist-receptor complex from the cytoskeleton, thereby activating adenylate cyclase.The importance of the f3-adrenergic receptor system derives from its ubiquitous distribution. The 13-receptor is functionally involved in regulation of processes as diverse as epithelial chloride transport (1) and central nervous system activity (2).It has long been clear that the fl-adrenergic receptor operates through an associated adenylate cyclase, which activity the receptor modulates (3). The molecular mechanism of the receptor-cyclase linkage has been the subject of considerable speculation, and although a number of hypotheses have been advanced, none have been validated or repudiated. Consequently, it is of considerable interest to employ receptor-specific biophysical probes, which allow clarification of the intimate details of the linkage.Propranolol, 1-(isopropylamino)-3-naphthyloxy-2-propanol, is a well-characterized antagonist for the ,3-adrenergic receptor, to which it binds with high affinity (4-6). Its availability as a tritium-labeled compound has made possible its utilization in the characterization of the receptor.The naphthalene nucleus of propranolol makes it a likely fluorescent substrate for the ,B-adrenergic receptor. Many compounds of this general structural type have been applied in the past as fluorescent probes in biological systems (7), and propranolol itself has been shown to fluoresce. This property has in fact been utilized in various pharmacokinetic studies (8,9). We have previously shown (10, 11) that the propranolol fluorescence is sufficiently intense to allow useful determinationThe publication costs of this article were defrayed in part by page char...

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Fluidity of LM cell membranes with modified lipid compositions as determined with 1,6-diphenyl-1,3,5-hexatriene

Cited by 62 publications

References 45 publications

Effect of fatty acids on plasma membrane lipid dynamics and cation permeability in neuroblastoma cells

Effect of fatty acids on plasma membrane lipid dynamics and cation permeability in neuroblastoma cells

Characterization of lipid domains in erythrocyte membranes.

Cytoskeletal constraint of the beta-adrenergic receptor in frog erythrocyte membranes.

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