Fluidity of the lipid phase of bovine serum high density lipoprotein from fluorescence polarization measurements

Jonas, Ana; Jung, Richard W.

doi:10.1016/0006-291x(75)90559-8

Cited by 23 publications

(3 citation statements)

References 12 publications

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“…One important question with these various P450s is the potential role of specific phospholipids in controlling their metabolic activities. Some early investigations with membrane reconstitution systems suggested that trace amounts of phospholipids were required for optimal activity (80), while others suggested that the presence of specific phospholipids might stabilize some kinetically distinguishable conformations of P450s, such as the major drug-metabolizing isoform CYP3A4, and directly modulate its affinity for particular substrates (81, 82). Subsequent recent investigations (71) attempted to define the role of specific phospholipids in maintaining high catalytic turnover.…”

Section: Nanodiscs For Analysis Of Lipid-protein Interactionsmentioning

confidence: 99%

Nanodiscs as a New Tool to Examine Lipid–Protein Interactions

Schuler

Denisov

Sligar

2012

Methods in Molecular Biology

139

131

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Nanodiscs are self-assembled discoidal fragments of lipid bilayers 8 – 16 nm in diameter, stabilized in solution by amphipathic helical scaffold protein. As stable and highly soluble membrane mimetics with controlled lipid composition and ability to add affinity tags to the scaffold protein, Nanodiscs represent an attractive model system for solubilization, isolation, purification, and biophysical and biochemical studies of membrane proteins. We overview various approaches to the structural and functional studies of different classes of integral membrane proteins such as ion channels, transporters, GPCR and other receptors, membrane enzymes, blood coagulation cascade proteins, et cetera incorporated into Nanodiscs with the special focus on the advantages provided by homogeneity, ability to control oligomerization state of the target protein and lipid composition of the bilayer. Special attention is paid to the opportunities provided by Nanodisc system for the detailed studies of the role of different lipid properties and protein – lipid interactions in the functional behavior of membrane proteins.

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Section: Nanodiscs For Analysis Of Lipid-protein Interactionsmentioning

confidence: 99%

Nanodiscs as a New Tool to Examine Lipid–Protein Interactions

Schuler

Denisov

Sligar

2012

Methods in Molecular Biology

139

131

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“…Although the results from [2H3]CO/egg PC/apoHDL3 and [2H6]TO/egg PC/apoHDL3 cannot be directly extrapolated to native HDL because of the simplicity of the model systems, the fatty acid composition of the phospholipid monolayers is similar (Scanu, 1979;Forrest, 1977). Therefore, it is possible that the existence of a state of disordered ester in native HDL above 0 °C (Tall et al, 1977;Jonas & Jung, 1975) can be partly explained on the basis of monolayer-core interactions. The greater compositional heterogeneity of the native system will also contribute to the disordering.…”

Section: Discussionmentioning

confidence: 99%

Lipid-lipid interactions in reconstituted high-density lipoproteins by deuterium nuclear magnetic resonance

Fenske¹,

Parmar²,

Treleaven³

et al. 1988

Biochemistry

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Lipid-lipid interactions between the core and monolayer have been studied by using reconstituted high-density lipoproteins (rHDLs) composed of apoHDL3 with either dipalmitoylphosphatidylcholine (DPPC) or egg phosphatidylcholine (egg PC) as the monolayer and either cholesteryl oleate (CO) or triolein (TO) as the core. The effect of the monolayer on the core was observed by deuterium nuclear magnetic resonance (2H NMR) studies of rHDLs containing the core component cholesteryl [18, 18, 18-2H3] oleate [( 2H3]CO) or tri[16,16-2H2]oleoylglycerol [( 2H6]TO) surrounded by a monolayer of either DPPC or egg PC as a function of temperature. The reverse effect, that of the core on the monolayer, was examined by both 2H and 31P NMR studies of rHDLs containing [5,5-2H2]PC in the presence of CO or TO as a function of temperature. The 2H NMR line widths of [2H3]CO and [2H6]TO were considerably broader and showed a greater temperature dependence in rHDLs containing DPPC than in those containing egg PC. Similarly, the C-2H order parameters of [2H2]PC were higher and showed a greater temperature dependence in rHDLs containing CO than in those containing TO. In contrast, the 31P NMR line widths were identical for both [2H2]-PC/CO/apoHDL3 and [2H2]PC/TO/apoHDL3 at 25 and 6 degrees C, showing only a slight temperature dependence. Thus, acyl chains of both the monolayer and core components show increased order when in contact with neighboring lipids of higher order. The data demonstrate a direct effect of core cholesteryl esters and triglycerides with the phospholipid monolayer of HDL.

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“…One important question with these various P450s is the potential role of specific phospholipids in controlling their metabolic activities. Some early investigations with membrane reconstitution systems suggested that trace amounts of phospholipids were required for optimal activity (80), while others suggested that the presence of specific phospholipids might stabilize some kinetically distinguishable conformations of P450s, such as the major drug-metabolizing isoform CYP3A4, and directly modulate its affinity for particular substrates (81,82). Subsequent recent investigations (71) attempted to define the role of specific phospholipids in maintaining high catalytic turnover.…”

Section: Protein-protein Interactions Using Nanodiscsmentioning

confidence: 99%

Nanodiscs as a New Tool to Examine Lipid–Protein Interactions

Denisov

Schuler

Sligar

2019

Methods in Molecular Biology

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Nanodiscs are self-assembled discoidal fragments of lipid bilayers 8-16 nm in diameter, stabilized in solution by amphipathic helical scaffold protein. As stable and highly soluble membrane mimetics with controlled lipid composition and ability to add affinity tags to the scaffold protein, Nanodiscs represent an attractive model system for solubilization, isolation, purification, and biophysical and biochemical studies of membrane proteins. We overview various approaches to the structural and functional studies of different classes of integral membrane proteins such as ion channels, transporters, GPCR and other receptors, membrane enzymes, blood coagulation cascade proteins, et cetera incorporated into Nanodiscs with the special focus on the advantages provided by homogeneity, ability to control oligomerization state of the target protein and lipid composition of the bilayer. Special attention is paid to the opportunities provided by Nanodisc system for the detailed studies of the role of different lipid properties and protein-lipid interactions in the functional behavior of membrane proteins.

show abstract

Fluidity of the lipid phase of bovine serum high density lipoprotein from fluorescence polarization measurements

Cited by 23 publications

References 12 publications

Nanodiscs as a New Tool to Examine Lipid–Protein Interactions

Nanodiscs as a New Tool to Examine Lipid–Protein Interactions

Lipid-lipid interactions in reconstituted high-density lipoproteins by deuterium nuclear magnetic resonance

Nanodiscs as a New Tool to Examine Lipid–Protein Interactions

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