Flunitrazepam induces geometrical changes at the lipid–water interface

A Comparative Study on DMSO-Induced Modulation of the Structural and Dynamical Properties of Model Bilayer Membranes

“…The FLIM images of the DMPC vesicles in pure water and in 5, 10, 14, 17, and 20 mol % DMSO are shown in Figure a. It has been previously reported that the curvature changes of the lipid membranes may be achieved through the changes in the environmental asymmetries such as a change in the pH or binding of the foreign molecules to the outermost surface of the lipid bilayers. − This is consistent with the decrease in the size of the vesicles and an increase in the surface curvature. The curvature changes can be interpreted as a mechanism suitable to compensate for the tension that originated due to the presence of DMSO inside the bilayer and may be the result of the dynamic interactions of many molecular fluxes of lipid molecules between two leaflets changing their lipidic composition to satisfy the spontaneous membrane curvature.…”

Formation of lipid membrane-incorporated small π-molecules bearing hydrophilic groups

“…35,36 Furthermore, it has been reported that the incorporation of some guest molecules can lead to a decrease in the diameter of the liposome. 37,38 Such decreases in the diameter can be caused by local increases in the curvature of the liposome, which occur as a geometric consequence of the incorporation of a cone-like guest molecules into a phospholipid bilayer. We analyzed the LMIGs in category (iii) by dynamic light scattering (DLS) and cryogenic transmission electron microscopy (cryo-TEM) analyses to determine whether the small aggregates formed in these cases were micelles or very small liposomes.…”

Lipophilicity of some GABAergic phenols and related compounds determined by HPLC and partition coefficients in different systems

“…Liposomes were prepared by evaporating, under reduced pressure, a chloroform solution of lipids (lecithin or dpPC) as described before [21]. The dry lipid was suspended in buffer Tris-HCl 50 mM pH 7.4 by repeating six consecutive cycles of heating at 50 • C for 2 min and vortexing for 30 s. Before use, the suspension was diluted to a final lipid concentration of 3.75 mg/ml, and each phenolic compound was added at 0.5-1 mM (final concentration).…”