Fluorene-9-bisphenol exposure induces cytotoxicity in mouse oocytes and causes ovarian damage

Jia, Zhenzhen; Wang, Hongyu; Feng, Zeyang; Zhang, Shaozhi; Wang, Lining; Zhang, Jingwen; Liu, Qianqian; Zhao, Xin; Feng, Daofu; Feng, Xisheng

doi:10.1016/j.ecoenv.2019.05.019

Cited by 47 publications

(13 citation statements)

References 57 publications

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“…This was similar to the results obtained when endosulfan was added to Sertoli germ cells ( Rastogi et al, 2014 ). In contrast to the results in this study, BPFL was found to induce increased expression of the autophagosome-forming protein LC3B in oocytes, which leads to the failure of in vitro maturation of oocytes ( Jia et al, 2019 ; Jiao et al, 2019 ). The effect of BPFL on autophagy may be due to the cell type or other potential molecular mechanisms, which will be studied in the future.…”

Section: Discussioncontrasting

confidence: 99%

“…In the present study, by detecting the ROS content, we found that the content of ROS was increased after BPFL exposure. Our findings were consistent with those of Jia et al (2019) , who found that BPFL-exposed oocytes accumulate excess ROS and undergo oxidative stress, apoptosis, and ultimately an inability to mature.…”

Section: Discussionsupporting

confidence: 92%

“…In these subsequent experiments, we found that BPFL exposure inhibits proliferation and induces apoptosis, thus impairing the in vitro cultured SCs. Previous studies have shown that porcine oocytes exposed to BPFL undergo early apoptosis, indicating that BPFL hinders oocyte maturation and reduces oocyte quality by inducing early apoptosis (Jia et al, 2019;Jiao et al, 2020). Our results also showed that BPFL induced apoptosis, suggesting that BPFL may have a negative effect on male fertility, raising our interest in further exploration.…”

Section: Discussionsupporting

confidence: 65%

“…BPFL (C 25 H 18 O 2 ), also known as BHPF, is a kind of bisphenol used in the plastics industry that has estrogen-like activity and antiandrogen effects, similar to BPA and BPE ( den Braver-Sewradj et al, 2020 ). At present, research on the reproductive toxicity of BPFL is mainly focused on oocytes or embryos from pigs, mice, and zebrafish ( Jia et al, 2019 ; Jiao et al, 2019 ; Jiao et al, 2020 ). In addition, a recent study of humans who spend considerable time in dense industrial areas showed that their mean serum concentration of BPFL was 0.578 ng/ml and that serum BPFL was significantly correlated with the levels of the oxidative stress indices MDA and 8-OHdG.…”

Section: Introductionmentioning

confidence: 99%

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Chlorogenic Acid Ameliorates Damage Induced by Fluorene-9-Bisphenol in Porcine Sertoli Cells

et al. 2021

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4,4′-(9-Fluorenylidene) diphenol (BPFL, also known as BHPF and fluorene-9-bisphenol) is a novel bisphenol A substitute that is used in the plastics industry as an organic synthesis intermediate and is a potential endocrine disruptor. However, the deleterious effects of BPFL on porcine Sertoli cells (SCs) and the possible underlying mechanisms are still unclear. Chlorogenic acid (CA) is a free radical scavenger in the cellular antioxidant system that prevents oxidative damage and apoptosis. In the present research, we found that BPFL induced impairments in porcine SCs in a dose-dependent manner and that CA protected porcine SCs against BPFL exposure-induced impairments. Cell viability, proliferation and apoptosis assay results revealed that BPFL exposure could inhibit porcine SC proliferation and induce apoptosis, while CA supplementation ameliorated the effects of BPFL. Further analysis revealed that BPFL exposure induced oxidative stress, mitochondrial membrane potential dysfunction and DNA damage accumulation. Transcriptome analysis and further real-time quantitative PCR and Western blot results showed that BPFL exposure induced endoplasmic reticulum stress and apoptosis. Supplementation with CA dramatically ameliorated these phenotypes in BPFL-exposed porcine SCs. Overall, the present research reveals the possible underlying mechanisms by which BPFL exposure induced impairments and CA supplementation protected against these impairments in porcine SCs.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

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Chlorogenic Acid Ameliorates Damage Induced by Fluorene-9-Bisphenol in Porcine Sertoli Cells

et al. 2021

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“…Proliferating cell nuclear antigen can be a marker and promoter of both ovarian cell proliferation [ 20 ] and oocyte maturation [ 21 , 22 ]. Caspase 3 can be both a marker and promoter of autophagy and apoptosis in ovarian [ 16 ] and non-ovarian [ 23 ] cells and in oocytes [ 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Dietary Supplementation of Flaxseed (Linum usitatissimum L.) Alters Ovarian Functions of Xylene-Exposed Mice

Vlčková

Andrejčáková

Lecová

et al. 2022

Life

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The aim of the performed study was to examine the ability of xylene, flaxseed, and their combinations to affect morphological and endocrine indexes of murine ovaries. The 72 indexes of secondary and tertiary follicular cells, oocytes, corpora lutea, and ovarian stroma have been quantified: diameter, markers of proliferation PCNA and apoptosis caspase 3, receptors to FSH, oxytocin, estrogen (alpha and beta), and progesterone. In addition, concentrations of the ovarian hormones progesterone, estradiol, and IGF-I in the blood, as well as their production by isolated ovaries cultured with and without gonadotropins (FSH + LH mixture), were determined using histological, immunohistochemical, and immunoassay analyses. The character of xylene and flaxseed effects on ovarian functions in mice depended on the stage of ovarian folliculogenesis. It was shown that flaxseed could mitigate and prevent the major (63%) effects of xylene on the ovary. In addition, the ability of gonadotropins to affect ovarian hormone release and prevent its response to xylene has been shown. The effects of these additives could be mediated by changes in the release and reception of hormones. These observations suggest that flaxseed and possibly gonadotropins could be natural protectors of a female reproductive system against the adverse effects of xylene.

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Electrical sensing of single oocytes in microfluidic systems: A mini review

Schnakenberg,

Cao

2024

Applied Research

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The non‐invasive and label‐free electrical characterization of single cells is increasingly gaining interest over the last years. Besides cancer cells, oocytes are in the focus of those investigations. The study of oocytes is relevant for two areas: on the one hand, the quality of mammalian oocytes is important in artificial reproduction technology for a high fertilization yield. On the other hand, amphibian oocytes are well established to be used as host cells for the characterization of ion‐channel kinetics for drug screening. For both areas, easy to perform and reliable characterization techniques are needed. This mini review summarizes important developments of characterizing oocytes with microfluidic systems in combination with observer‐independent non‐invasive electrical sensing. Such miniaturized microfluidic systems can be improved to facilitate multiple oocyte characterizations simultaneously for higher throughput screening.This article is protected by copyright. All rights reserved.

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Fluorene-9-bisphenol exposure induces cytotoxicity in mouse oocytes and causes ovarian damage

Cited by 47 publications

References 57 publications

Chlorogenic Acid Ameliorates Damage Induced by Fluorene-9-Bisphenol in Porcine Sertoli Cells

Chlorogenic Acid Ameliorates Damage Induced by Fluorene-9-Bisphenol in Porcine Sertoli Cells

Dietary Supplementation of Flaxseed (Linum usitatissimum L.) Alters Ovarian Functions of Xylene-Exposed Mice

Electrical sensing of single oocytes in microfluidic systems: A mini review

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