Fluorescein-based ionic liquid sensor for label-free detection of serum albumins

Galpothdeniya, Waduge Indika S.; Das, Susmita; Rooy, Sergio L. de; Regmi, Bishnu P.; Hamdan, Suzana; Warner, Isiah M.

doi:10.1039/c4ra01461h

Cited by 19 publications

(14 citation statements)

References 40 publications

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“…33 These materials also have potential as an alternative method of analyzing medical polymers via fluorescent microscopy, as well as for chemical sensors or for monitoring degradation. 58 Future studies will examine the anti-microbial and degradation properties of these fluorescent SMPs. Additionally, this controlled behaviour could be useful for incorporating drug release capabilities, as demonstrated in similar studies using photothermal release from outside of the tissue, or for sensing changes in the in vivo environment.…”

Section: Resultsmentioning

confidence: 99%

Shape memory polymers with visible and near-infrared imaging modalities: synthesis, characterization and in vitro analysis

et al. 2017

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Shape memory polymers (SMPs) are promising for non-invasive medical devices and tissue scaffolds, but are limited by a lack of visibility under clinical imaging. Fluorescent dyes are an alternative to radiocontrast agents in medical applications, they can be utilized in chemical sensors and monitors and may be anti-microbial agents. Thus, a fluorescent SMP could be a highly valuable biomaterial system. Here, we show that four fluorescent dyes (phloxine B (PhB), eosin Y (Eos), indocyanine green(IcG), and calcein (Cal)) can be crosslinked into the polymer backbone to enhance material optical properties without alteration of shape memory and thermomechanical properties. Examinations of the emission wavelengths of the materials compared with the dye solutions showed a slight red shift in the peak emissions, indicative of crosslinking of the material. Quantitative analysis revealed that PhB enabled visibility through 1 cm of blood and through soft tissue. We also demonstrate the utility of these methods in combination with radio-opaque microparticle additives and the use of laser-induced shape recovery to allow for rapid shape recovery below the glass transition temperature. The crosslinking of fluorescent dyes into the SMP enables tuning of physical properties and shape memory and independently of the fluorescence functionality. This fluorescent SMP biomaterial system allows for use of multiple imaging modalities with potential application in minimally invasive medical devices.

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Section: Resultsmentioning

confidence: 99%

Shape memory polymers with visible and near-infrared imaging modalities: synthesis, characterization and in vitro analysis

et al. 2017

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“…In comparison with other detection methods, fluorescent probes are favored because of advantages including a rapid, non-invasive, and sensitive response. Accordingly, several fluorescent probes have been reported using many different types of fluorophore such as tetraphenylethylene, squaraine dye, and indolium derivative, which is based on aggregation-induced emission (AIE), molecular rotor containing donor-π-acceptor (D-π-A) structured fluorophore, and self-assembled nanoparticles [22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37]. However, there remain some problems to be solved, such as the low sensitivity and selectivity toward HSA.…”

Section: Introductionmentioning

confidence: 99%

Development of Human Serum Albumin Selective Fluorescent Probe Using Thieno[3,2-b]pyridine-5(4H)-one Fluorophore Derivatives

Lee

Sung

Kang

et al. 2019

Sensors

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The level of human serum albumin (HSA) in biological fluids is a key health indicator and its quantitative determination has great clinical importance. In this study, we developed a selective and sensitive fluorescent HSA probe by fluorescence-based high-throughput screening of a set of fluorescent thieno[3,2-b]pyridine-5(4H)-one derivatives against major plasma proteins: HSA, bovine serum albumin (BSA), globulin, fibrinogen, and transferrin. The fluorophore chosen finally (4) showed noticeable fluorescence enhancement in the presence of HSA (160-fold increase), and it exhibited rapid response, high sensitivity (detection limit 8 nM), and the ability to clearly distinguish HSA from BSA in pH 9 buffer condition. Moreover, the probe could be applicable to detect trace amounts of HSA in an artificial urine sample; further, it might be applied to the determination of the HSA concentration in complex biological samples for pre-clinical diagnosis.

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“…128 These compounds proved slightly less conductive than their cyclic ammonium congeners. Warner and co-workers described a new class of PILs obtained from anionic fluorescein derivatives paired to phosphonium cations 129 These PILs were fluorescent probes for highly selective and sensitive detection of albumins. The synthesis and detailed characterization of tetraalkyl phosphonium oxalate ionic liquids were described by Quiroz-Guzman et al 130 Metal-containing PILs can also be prepared.…”

Section: Preparation and Characterisationmentioning

confidence: 99%

Phosphonium salts and P-ylides

Selva¹,

Perosa²,

Fiorani³

2020

Organophosphorus Chemistry

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Fluorescein-based ionic liquid sensor for label-free detection of serum albumins

Abstract: Fluorescein-based room temperature ionic liquid as a fluorescent probe for highly selective and sensitive detection of serum albumins.

Cited by 19 publications

References 40 publications

Shape memory polymers with visible and near-infrared imaging modalities: synthesis, characterization and in vitro analysis

Shape memory polymers with visible and near-infrared imaging modalities: synthesis, characterization and in vitro analysis

Development of Human Serum Albumin Selective Fluorescent Probe Using Thieno[3,2-b]pyridine-5(4H)-one Fluorophore Derivatives

Phosphonium salts and P-ylides

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