Fluorescein Diacetate: A Potential Biological Indicator for Arid Soils

Aseri, Gajender Kumar; Tarafdar, J. C.

doi:10.1080/15324980500544473

Cited by 29 publications

(13 citation statements)

References 22 publications

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“…Soil enzyme assays have been used to investigate biochemical processes such as soil organic matter formation and degradation, nutrient cycling and interaction between inoculants and indigenous microbial population in soils and is a possible integrative measure of soil quality to reflect biological status of soils [4,30]. Fluorescein diacetate activity was considered in the present study as it was reported to be a better biological indicator of soils than dehydrogenase activity [31].…”

Section: Resultsmentioning

confidence: 99%

Phytase, Phosphatase Activity and P-Nutrition of Soybean as Influenced by Inoculation of Bacillus

et al. 2011

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The efficiency of different Bacillus isolates on rhizosphere soil enzyme activities and P-nutrition of soybean was carried out under microcosm conditions. Significant increase in enzyme activities viz., fluorescein diacetate activity, phosphatase and phytase activity and consequent effects on P-nutrition were observed with the inoculation of Bacillus isolates over uninoculated control. Among the isolates, BD-3-1B, KHBD-6, BDKH-3, Bacillus amyloliquefacians, and Bacillus cereus were found to be promising. The phytic acid-P as a percentage of total P content in soybean seeds decreased with the inoculation of Bacillus isolates as compared to un-inoculated control. A decrease in phytic-P in soybean seeds not only results in better digestibility and increased feed efficiency. Pearson correlation studies revealed a significant positive association between acid, alkaline phosphatases, phytase activity on available P content in soil and P content in seeds with the inoculation of Bacillus isolates, indicating role of these enzymes in P mobilization and acquisition by soybean.

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Section: Resultsmentioning

confidence: 99%

Phytase, Phosphatase Activity and P-Nutrition of Soybean as Influenced by Inoculation of Bacillus

et al. 2011

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“…Kumar and Tarafdar [17] found that dehydrogenase activity is mainly contribution of bacteria and actinomycetes in arid soils and only 5 % by the fungal activity. It is clear that DHA is an indicator of bacterial activity in soil, whereas FDA hydrolysis is the indicator for measuring overall microbial activity as it is govern by lipase, protease and esterase enzymes originating from all microbial sources [4]. From the results of FDA and ALP, it seems that there is some relationship between FDA and ALP that might be due to more fungal population in black soil (Vertisols) as compared to arid soil [4,17], where they reported a significant relationship between DHA and ALP might be due to lower fungal population.…”

Section: Discussionmentioning

confidence: 99%

“…Fluorescein diacetate hydrolysis activity in soil was determined by hydrolyzing the FDA [4] using 60 mM potassium phosphate buffer of pH 7.6. The soil dehydrogenase activity was determined by the method of Cassida [7].…”

Section: Growing Conditions and Measurementsmentioning

confidence: 99%

Effect of Native Soybean Rhizobia and AM Fungi in the Improvement of Nodulation, Growth, Soil Enzymes and Physiological Status of Soybean Under Microcosm Conditions

et al. 2012

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The arbuscular mycorrhizal (AM) fungi-rhizobia synergism is a promising approach for improving the growth and nutrition of soybean. It is, therefore, imperative to evaluate potential soybean rhizobia and AM fungi singly to identify their stress protectant physiological traits, enhance growth and nodulation of soybean and improve soil health. The efficacy of five root nodulating soybean rhizobia and an indigenous AM fungus, Glomus intraradices, was evaluated on soybean (cv JS 93-05) under microcosm conditions. In general, all the inoculated plants showed higher fresh shoot and root weight, and nodule number as compared to uninoculated control plants. The plants inoculated with Bradyrhizobium japonicum (strain USDA 110), B. liaoningense 17c (MTCC 10753) and AM fungus showed higher growth and nodulation. However, the plants inoculated with rhizobia 12c (unidentified), B. japonicum DE2-5a (MTCC 10751) and USDA 205 did enhance nodulation but found at par with the other inoculated plants. Interestingly, these inoculated plants found to have comparatively higher nitrogen and phosphorus uptake. B. japonicum (strain USDA 110), native slow growing rhizobia isolate DE2-5a and AM fungi were also found to stimulate proline content in shoots, and trehalase and fluorescein diacetate activities in the rhizosphere soil. Considering the growth and physiological responses of AM fungi and rhizobial strains (DE2-5a and 17c) to soybean, there is a need for further testing the synergistic responses to evolve better survival candidates under drought-stress conditions.

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“…However, to have the exact magnitude of the pollution of environment, the physico-chemical analysis has its own importance. Some scientists have, therefore, studied the soil ecosystem by analysis of various physico-chemical properties like pH, alkalinity, soil texture, water holding capacity, bulk density, nitrates, phosphates, potassium, calcium, sodium, magnesium, chlorides and lead (Garcia et al, 2001;Garg, 2002;Kulkarni and aggarwal, 2003;Aseri and Tarafdar, 2006;Glori et al, 2008 andKatnoria et al, 2011). Considering the increased exposure of people to traffic emissions in Amritsar, Punjab (India), the present study was planned to estimate the genotoxic and tumor inducing potential of surface soil samples exposed to vehicular emissions.…”

Section: Introductionmentioning

confidence: 99%

Genotoxicity and tumor inducing potential of roadside soil samples exposed to heavy traffic emissions at Amritsar (Punjab), India

Kaur

Pakade

Katnoria

2013

JANS

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Noxious gases released from vehicles adversely affect the microbial population of the roadside soil as well as exerts influence on plant growth and development. Considering the increasing vehicular pollution in Amritsar, a holy city of Punjab (India) and their serious consequences in terms of health problems, the present study was planned to estimate genotoxic and tumor inducing potentials of soil samples from Golden temple (GT) and Putlighar Chowk (PG) by employing Allium cepa root chromosomal aberration assay (AlRCAA) and Potato disc tumor assay (PDTA), respectively. The genotoxic potential in terms of percent aberrant cells in A. cepa was found to be 29.24 % (GT) and 29.24 % (PG) during in situ treatment and 39.00 % (GT) and 39.48 % (PG) during root dip treatment. The average number of tumors was found to be 14.4 in PG sample, followed by 13.0 in GT sample. Both samples have shown high content of lead viz., 39.81 (GT) and 56.48 (PG) during physicochemical analysis of the samples.

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Fluorescein Diacetate: A Potential Biological Indicator for Arid Soils

Cited by 29 publications

References 22 publications

Phytase, Phosphatase Activity and P-Nutrition of Soybean as Influenced by Inoculation of Bacillus

Phytase, Phosphatase Activity and P-Nutrition of Soybean as Influenced by Inoculation of Bacillus

Effect of Native Soybean Rhizobia and AM Fungi in the Improvement of Nodulation, Growth, Soil Enzymes and Physiological Status of Soybean Under Microcosm Conditions

Genotoxicity and tumor inducing potential of roadside soil samples exposed to heavy traffic emissions at Amritsar (Punjab), India

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