Fluorescein staining and physiological state of corneal epithelial cells

Bandamwar, Kalika L.; Papas, Eric; Garrett, Qian

doi:10.1016/j.clae.2013.11.003

Cited by 46 publications

(44 citation statements)

References 31 publications

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“…This is a conclusion also reached by Bandamwar et al (2014). We have proposed, too, that access requires a loss of components of the protective apical glycocalyx, which, as noted, has been demonstrated in the rat cornea .…”

Section: Transcellular Uptake Of Dye and Intercellular Spreadsupporting

confidence: 73%

“…Norn had earlier observed in the human, that shed epithelial cells, trapped in the fornical mucus, stained rapidly and positively with rose bengal (Norn, 1969). Bandamwar et al (2012Bandamwar et al ( , 2014 have presented evidence that staining cells are those undergoing apoptosis while preparing for shedding.…”

Section: Fluorescein Sodiummentioning

confidence: 96%

“…Such cells were not dead cells and rarely stain with PI (propidium iodide) (Bakkar et al, 2014). Studies by Bandamwar et al (2014) suggest that such cells are undergoing apoptosis. The fraction of hyperfluorescent cells is increased by MPS exposure without an increase in cell death (Bakkar et al, 2014).…”

Section: Fluorescein Spread Within the Epitheliummentioning

confidence: 99%

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Clinical staining of the ocular surface: Mechanisms and interpretations

Bron

Argüeso

İrkeç

et al. 2015

Progress in Retinal and Eye Research

140

109

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Section: Transcellular Uptake Of Dye and Intercellular Spreadsupporting

confidence: 73%

Section: Fluorescein Sodiummentioning

confidence: 96%

See 1 more Smart Citation

Clinical staining of the ocular surface: Mechanisms and interpretations

Bron

Argüeso

İrkeç

et al. 2015

Progress in Retinal and Eye Research

140

109

View full text Add to dashboard Cite

“…6 This was recently questioned by Bandamwar et al, 7 who showed in vitro and ex vivo with an animal model (New Zealand white rabbit model) that damaged, apoptotic, or dying epithelial cells take up fluorescein at a high rate, and suggested that punctate staining of the corneal epithelium corresponded to the fluorescence of apoptotic cells. 8 In this study, the size of these cells on ex vivo rabbit corneal epithelium was approximately 20 · 40 mm. This demonstration is consistent with the findings of Wilson et al, 9 who also showed that fluorescein staining was because of staining of individual cells.…”

Section: Discussionmentioning

confidence: 67%

Size of the Lesions of Superficial Punctate Keratitis in Dry Eye Syndrome Observed With a Slit Lamp

Courrier¹,

Lépine

Hor

et al. 2016

Cornea

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“…Microscope images and levels of fluorescence intensities were observed and quantified using ImageJ software, as described previously. 25 Briefly, each oocyte was analyzed by its fluorescence intensity. After establishing a base value of intensity, the oocytes were classified according to the fluorescence intensity based on its response to HO or PI using ImageJ analysis software.…”

Section: Oocyte Integritymentioning

confidence: 99%

Maternal Overweight Disrupts the Sexual Maturation of the Offspring

et al. 2017

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The aims of the present work were to study the effect of maternal overweight and obesity on the ovarian reserve, follicular development, and ovulation of the offspring and to assess whether this maternal condition alters oocyte integrity. To this end, female offspring from rats fed standard (OSD) or cafeteria (OCD) diet were used. Body weight, vaginal opening, and estrous cycle were recorded and ovaries were obtained on the day of the second estrus. In addition, ovarian weight, ovulation rate (measured by the number of oocytes within oviducts), follicular development (determined by histology), and oocyte integrity were examined. The OCD were divided into 2 groups: offspring from rats with 17% and 28% of overweight (OCD17 and OCD28, respectively). Both OCD groups showed higher body weight, but OCD28 also exhibited early vaginal opening and higher ovarian weight and glycemia at euthanasia compared with OSD. Both OCD17 and OCD28 had lower number of primordial and primary follicles, and only OCD28 exhibited lower number of antral follicles, all compared with OSD rats. In addition, both OCD17 and OCD28 had higher ovulation rate than controls, and OCD28 had lower number of healthy oocytes, which, in turn, exhibited morphological alterations such as larger perivitelline space and zona pellucida than those of control animals. These results suggest that maternal overweight may severely affect the reproductive ability of the offspring, likely as a result of altering the organogenesis.

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Fluorescein staining and physiological state of corneal epithelial cells

Cited by 46 publications

References 31 publications

Clinical staining of the ocular surface: Mechanisms and interpretations

Clinical staining of the ocular surface: Mechanisms and interpretations

Size of the Lesions of Superficial Punctate Keratitis in Dry Eye Syndrome Observed With a Slit Lamp

Maternal Overweight Disrupts the Sexual Maturation of the Offspring

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