Fluorescence and fluorescence quenching

Bowen, E. J.

doi:10.1039/qr9470100001

Cited by 16 publications

(8 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Dynamic quenching takes place when the fluorophore's emission intensity and fluorescence lifetime decrease equivalently due to collisions with other molecules in its environment. 2,19 These processes do not result in any chemical changes of the fluorophore or quenching group. The efficiency of dynamic quenching is hence sensitively dependent on the concentration and diffusion of the quenching species.…”

Section: Fluorophores and Fluorescencementioning

confidence: 99%

Fluorescence Activation and Enhancement with Photogenerated Radicals, Pre-fluorescent Probes and Silver Nanostructures

Golian

2024

Preprint

View full text Add to dashboard Cite

<p>This thesis proposes a boron-dipyrromethene (BODIPY) fluorophore-based radical sensing system covalently linked to 2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO), a paramagnetic nitroxide, known to quench fluorescence non-radiatively through electron exchange. Introduction of a free radical creates a diamagnetic product, resulting in the restoration of fluorescence due to the inhibition of its quenching pathway. This has been successfully applied to the activation of a pre-fluorescent system with the addition of metal enhanced fluorescence via silver nanoparticles. Further research looks at extending the π conjugation of the BODIPY based pre-fluorescent probe to bathochromically shift its spectroscopic properties to enhance its potential in biological monitoring applications.</p>

show abstract

Section: Fluorophores and Fluorescencementioning

confidence: 99%

Fluorescence Activation and Enhancement with Photogenerated Radicals, Pre-fluorescent Probes and Silver Nanostructures

Golian

2024

Preprint

View full text Add to dashboard Cite

show abstract

“…It does not simultaneously guarantee the emission of a fluorescent signal, which is only observed for a small fraction of absorbers due to the action of prominent quenching mechanisms, such as internal conversion (IC) or intersystem crossing (ISC). 44 Fluorescence is favored over absorption as the signalgenerating mode due to a better signal-to-noise ratio and thus lower detection limits. Generally, it is easier to see a strong signal against a weak background than to look for weakening of a strong background.…”

Section: Principles For Designing Functional Group Probesmentioning

confidence: 99%

“…Extension of a conjugated π–electron system upon reaction is a common element in designing chromogenic probes, which generally leads to a bathochromic shift in the absorption maximum. It does not simultaneously guarantee the emission of a fluorescent signal, which is only observed for a small fraction of absorbers due to the action of prominent quenching mechanisms, such as internal conversion (IC) or intersystem crossing (ISC) . Fluorescence is favored over absorption as the signal-generating mode due to a better signal-to-noise ratio and thus lower detection limits.…”

Section: Principles For Designing Functional Group Probesmentioning

confidence: 99%

Enlightening the Path to Protein Engineering: Chemoselective Turn-On Probes for High-Throughput Screening of Enzymatic Activity

et al. 2023

View full text Add to dashboard Cite

Many successful stories in enzyme engineering are based on the creation of randomized diversity in large mutant libraries, containing millions to billions of enzyme variants. Methods that enabled their evaluation with high throughput are dominated by spectroscopic techniques due to their high speed and sensitivity. A large proportion of studies relies on fluorogenic substrates that mimic the chemical properties of the target or coupled enzymatic assays with an optical read-out that assesses the desired catalytic efficiency indirectly. The most reliable hits, however, are achieved by screening for conversions of the starting material to the desired product. For this purpose, functional group assays offer a general approach to achieve a fast, optical read-out. They use the chemoselectivity, differences in electronic and steric properties of various functional groups, to reduce the number of false-positive results and the analytical noise stemming from enzymatic background activities. This review summarizes the developments and use of functional group probes for chemoselective derivatizations, with a clear focus on screening for enzymatic activity in protein engineering.

show abstract

“…The use of fluorescent techniques for monitoring ALP activity as a targeted tumor assay has great potential due to their intrinsic advantages such as noninvasiveness, sensitivity, and high solubility. Various mechanisms, such as assembly of nanoparticles, host–guest interactions, and change in solubility, can guide changes in the fluorescent behavior of bioreactive ALPs that can then be visualized both in vitro and in vivo . One of these mechanisms, the hydrolysis of 4-nitrophenyl phosphate (NPP) to 4-nitrophenol (NP), has been widely used to detect ALP activity in biological studies.…”

mentioning

confidence: 99%

“…Various mechanisms, such as assembly of nanoparticles, host−guest interactions, and change in solubility, can guide changes in the fluorescent behavior of bioreactive ALPs that can then be visualized both in vitro and in vivo. 8 One of these mechanisms, the hydrolysis of 4-nitrophenyl phosphate (NPP) to 4-nitrophenol (NP), has been widely used to detect ALP activity in biological studies.…”

mentioning

confidence: 99%

Determination of Cancer Cell-Based pH-Sensitive Fluorescent Carbon Nanoparticles of Cross-Linked Polydopamine by Fluorescence Sensing of Alkaline Phosphatase Activity on Coated Surfaces and Aqueous Solution

et al. 2017

View full text Add to dashboard Cite

The tumor-specific sensitive fluorescence sensing of cellular alkaline phosphatase (ALP) activity on the basis of host-guest specific and pH sensitivity was conducted on coated surfaces and aqueous states. Cross-linked fluorescent nanoparticles (C-FNP) consisting of β-cyclodextrin (β-CD)/boronic acid (BA) and fluorescent hyaluronic acid [FNP(HA)] were conjugated to fluorescent polydopamine [FNP(pDA)]. To determine the quenching effect of this system, hydrolysis of 4-nitrophenyl phosphate (NPP) to 4-nitrophenol (NP) was performed in the cavity of β-CD in the presence of ALP activated photoinduced electron transfer (PET) between NP and C-FNP. At an ALP level of 30-1000 U/L, NP caused off-emission of C-FNP because of their specific host-guest recognition. Fluorescence can be recovered under pH shock due to cleavage of the diol bond between β-CD and BA, resulting in release of NP from the fluorescent system. Sensitivity of the assays was assessed by confocal imaging not only in aqueous states, but also for the first time on coated surfaces in MDAMB-231 and MDCK cells. This novel system demonstrated high sensitivity to ALP through generation of good electron donor/acceptor pair during the PET process. Therefore, this fluorescence sensor system can be used to enhance ALP monitoring and cancer diagnosis on both coated surfaces and in aqueous states in clinical settings.

show abstract

Fluorescence and fluorescence quenching

Cited by 16 publications

References 1 publication

Fluorescence Activation and Enhancement with Photogenerated Radicals, Pre-fluorescent Probes and Silver Nanostructures

Fluorescence Activation and Enhancement with Photogenerated Radicals, Pre-fluorescent Probes and Silver Nanostructures

Enlightening the Path to Protein Engineering: Chemoselective Turn-On Probes for High-Throughput Screening of Enzymatic Activity

Determination of Cancer Cell-Based pH-Sensitive Fluorescent Carbon Nanoparticles of Cross-Linked Polydopamine by Fluorescence Sensing of Alkaline Phosphatase Activity on Coated Surfaces and Aqueous Solution

Contact Info

Product

Resources

About