Fluorescence Anisotropy Studies on Bodipy (Pyrromethene 546) Dye as a Novel Thermal Probe

Expanded single-color barcoding in microspheres with fluorescence anisotropy for multiplexed biochemical detection

“…For example, the FA of BODIPY inside the microspheres at room temperature could reach 0.29, which is much higher than the FA value of 0.004 in dilute MeOH solution. 29 The enhanced FA was attributed to the reduced wobblein-cone rotational motion of molecules inside the rigid internal structure of polystyrene microspheres. 30 Classical dyes with a high fluorescence quantum yield and high fundamental anisotropy such as DPH, 26 BODIPY, 27 and Nile Red 28 were employed as high FA components.…”

“…For example, the FA of BODIPY inside the microspheres at room temperature could reach 0.29, which is much higher than the FA value of 0.004 in dilute MeOH solution. 29 The enhanced FA was attributed to the reduced wobble-in-cone rotational motion of molecules inside the rigid internal structure of polystyrene microspheres. 30…”

Influence of Viscosity and Molecular Size on Temperature Sensitivity of Fluorescence Anisotropy

“…Consequently, the relaxation of FA is strongly influenced by the distribution of absorption and emission moments, the rotational Brownian motion of the molecules, and the transfer of excitation energy between fluorescent molecules. (12) Since rotational Brownian motion is temperature-dependent among the parameters that affect FA, it is possible to extract the temperature information of the molecules in the liquid from FA. FA, r, is defined as the degree of polarization of the fluorescence emission divided by the total fluorescence intensity as follows.…”

“…Our group has focused on the fluorescence anisotropy (FA) of fluorophores as an indicator of liquid temperature. (11)(12)(13) This technique analyzes the polarization characteristics of the fluorescence emission of a probe molecule. FA is independent of the solution pH (14) and is not affected by a nonuniform illumination intensity, molecular concentration, (15) or liquid film thickness, which may cause significant errors in LIF measurement.…”

A BODIPY‐Based Molecular Rotor in Giant Unilamellar Vesicles: A Case Study by Polarization‐Resolved Time‐Resolved Emission and Transient Absorption Spectroscopy

“…Giant unilamellar vesicles (GUVs) are suitable models for cellular membranes [12] . The rotational relaxation time, organization and orientation of various fluorophores (including BODIPYs) in GUVs as well as in cell membranes have been investigated previously [13–18] . A technique routinely used is time‐resolved emission anisotropy (TEA) [17‐20] .…”