Fluorescence assay for the determination of glutathione based on a ring-fused 2-pyridone derivative in dietary supplements

Kasprzyk, Wiktor; Koper, Filip; Flis, Agata; Szreder, Dominika; Pamuła, Elżbieta; Bogdał, Dariusz; Wybraniec, Sławomir; Ortyl, Joanna; Świergosz, Tomasz

doi:10.1039/d0an02245d

Cited by 12 publications

(3 citation statements)

References 47 publications

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“…These compounds were successfully studied in several applications. [70][71][72][73][74] In 2018, it was found that upon heating of mixtures of urea and citric or citrazinic acid under anhydrous conditions, a green-emitting fluorophore can be readily obtained, identified as (4-hydroxy-1H-pyrrolo [3,4-c]pyridine-1,3,6(2H,5H)-trione -HPPT). 40 This discovery led to many applications based on green emitting carbon dots containing HPPT on their surface.…”

Section: Cbmfsa Historical Perspectivementioning

confidence: 99%

The role of molecular fluorophores in the photoluminescence of carbon dots derived from citric acid: current state-of-the-art and future perspectives

et al. 2022

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Section: Cbmfsa Historical Perspectivementioning

confidence: 99%

The role of molecular fluorophores in the photoluminescence of carbon dots derived from citric acid: current state-of-the-art and future perspectives

et al. 2022

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“…The values of calculated atomic charges and bond lengths are presented in Table 2 , along with the corresponding changes in these values under electronic excitation S 0 → S 1 . From the obtained data, one can see that the total negative charge is distributed dominantly at the oxygen and nitrogen atoms, while the carbon atoms bear the appreciable positive charges because of the polarization of the C-O and C-N bonds [ 35 , 36 , 37 , 38 ]. The electronic excitation S 0 → S 1 of the anionic HPPT molecule causes noticeable redistribution of the electron occupancies at both atoms and bonds.…”

Section: Resultsmentioning

confidence: 99%

Nature of Linear Spectral Properties and Fast Electronic Relaxations in Green Fluorescent Pyrrolo[3,4-c]Pyridine Derivative

Башмакова

Dmytruk

Świergosz

et al. 2021

IJMS

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The electronic nature of 4-hydroxy-1H-pyrrolo[3,4-c]pyridine-1,3,6(2H,5H)-trione (HPPT) was comprehensively investigated in liquid media at room temperature using steady-state and time-resolved femtosecond transient absorption spectroscopic techniques. The analysis of the linear photophysical and photochemical parameters of HPPT, including steady-state absorption, fluorescence and excitation anisotropy spectra, along with the lifetimes of fluorescence emission and photodecomposition quantum yields, revealed the nature of its large Stokes shift, specific changes in the permanent dipole moments under electronic excitation, weak dipole transitions with partially anisotropic character, and high photostability. Transient absorption spectra of HPPT were obtained with femtosecond resolution and no characteristic solvate relaxation processes in protic (methanol) solvent were revealed. Efficient light amplification (gain) was observed in the fluorescence spectral range of HPPT, but no super-luminescence and lasing phenomena were detected. The electronic structure of HPPT was also analyzed with quantum-chemical calculations using a DFT/B3LYP method and good agreement with experimental data was shown. The development and investigation of new pyrrolo[3,4-c]pyridine derivatives are important due to their promising fluorescent properties and potential for use in physiological applications.

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“…In the last 20 years, several methods have been proposed for GSH determination, such as methods based on high-performance liquid chromatography (HPLC) (6), UV-Vis spectrophotometry (6), fluorescence spectroscopy (7), and mass spectrometry (8). Despite acceptable sensitivity, some of these methods suffer from practical disadvantages, such as the need for expensive and high-tech instrumentation, time-consuming processes, and complex sample preparation processes.…”

Section: Inroductionmentioning

confidence: 99%

Development of an Inhibition-Based Colorimetric Method For Glutathione Determination

Canbay,

Ceylan,

Topçu

et al. 2024

Journal of Immunology and Clinical Microbiology

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Aim: Glutathione (GSH, L-γ-glutamyl-L-cysteinyl-glycine), one of the major cellular antioxidants, is an important non-protein intracellular physiological antioxidant with sulphhydryl groups for detoxification of reactive oxygen species (ROS) in all living organisms. GSH deficiency has been shown to be associated with many human diseases, including cardiovascular, immune and ageing diseases, arthritis and diabetes. Therefore, the development of an accurate, reliable and sensitive method for the determination of GSH in biological fluids is essential for the understanding of GSH homeostasis in medicine and biochemical research. Materials and Methods : In this study, a very inexpensive, practical, rapid, sensitive and highly specific colourimetric method for the determination of glutathione (GSH) was developed that can be detected by the naked eye. The method is based on the inhibition of horseradish peroxidase (HRP) by GSH. As the concentration of glutathione increases, a pink coloured compound consisting of 4-chlorophenol, H2O2 and 4-aminoantipyrine (4-AAP) decomposes as a result of the reaction catalysed by HRP, thus reducing the intensity of the colour. Results: While the linear range of the developed method was found to be between 15.6-1000 M, the intra- and inter-day repeatability % coefficient of variation values of the method were less than 15%. The effect of potential interfering substances on the developed method was tested and no interference was found except for cysteine. Cysteine increased the response for GSH by 10%. The developed method was used for the determination of GSH in commercial serum samples and results were obtained between 91-106%. Conclusion: In conclusion, this study has developed a very simple, inexpensive and unique colourimetric method for the determination of GSH.

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Fluorescence assay for the determination of glutathione based on a ring-fused 2-pyridone derivative in dietary supplements

Abstract: Herein, a novel fluorescent method for the determination of GSH levels in aqueous solutions involving the utilization of citric acid as a derivatization reagent was developed.

Cited by 12 publications

References 47 publications

The role of molecular fluorophores in the photoluminescence of carbon dots derived from citric acid: current state-of-the-art and future perspectives

The role of molecular fluorophores in the photoluminescence of carbon dots derived from citric acid: current state-of-the-art and future perspectives

Nature of Linear Spectral Properties and Fast Electronic Relaxations in Green Fluorescent Pyrrolo[3,4-c]Pyridine Derivative

Development of an Inhibition-Based Colorimetric Method For Glutathione Determination

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