2023
DOI: 10.1021/acs.jpcb.3c06905
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Fluorescence Bar-Coding and Flowmetry Based on Dark State Transitions in Fluorescence Emitters

Elin Sandberg,
Baris Demirbay,
Abhilash Kulkarni
et al.

Abstract: Reversible dark state transitions in fluorophores represent a limiting factor in fluorescence-based ultrasensitive spectroscopy, are a necessary basis for fluorescence-based superresolution imaging, but may also offer additional, largely orthogonal fluorescence-based readout parameters. In this work, we analyzed the blinking kinetics of Cyanine5 (Cy5) as a barcoding feature distinguishing Cy5 from rhodamine fluorophores having largely overlapping emission spectra. First, fluorescence correlation spectroscopy (… Show more

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