Fluorescence-Based Molecular Imaging of Porcine Urinary Bladder Sentinel Lymph Nodes

Lee, Hak J.; Barback, Christopher V.; Hoh, Carl K.; Qin, Zhengtao; Kader, Karim; Hall, D.; Vera, David R.; Kane, Christopher J.

doi:10.2967/jnumed.116.178582

Cited by 19 publications

(11 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been evaluated in several animal models to date, and could identify SLNs of the hind paw of rats [ 14 ], rabbits [ 22 ], and prostate SLNs in dogs [ 23 ]. A more recent study performed by Lee, et al [ 24 ], tested this method for identification of sentinel lymph nodes real-time during robotic-assisted cystectomy in a porcine model. The study examined 36 lymph nodes, detecting fluorescent-labeled tilmanocept using a standard fluorescence imaging endoscope up to 38 hours after submucosal injection into the porcine bladder.…”

Section: Introductionmentioning

confidence: 99%

Molecular Imaging of endometrial sentinel lymph nodes utilizing fluorescent-labeled Tilmanocept during robotic-assisted surgery in a porcine model

et al. 2018

Self Cite

View full text Add to dashboard Cite

Molecular imaging with a fluorescent version of Tilmanocept may permit an accurate and facile detection of sentinel nodes of endometrial cancer. Tilmanocept accumulates in sentinel lymph nodes (SLN) by binding to a cell surface receptor unique to macrophages and dendritic cells. Four female Yorkshire pigs underwent cervical stromal injection of IRDye800-Tilmanocept, a molecular imaging agent tagged with near-infrared fluorescent dye and radiolabeled with gallium-68 and technetium-99m. PET/CT scans 1.5 hours post-injection provided pre-operative SLN mapping. Robotic-assisted lymphadenectomy was performed two days after injection, using the FireFly imaging system to identify nodes demonstrating fluorescent signal. After removal of fluorescent nodes, pelvic and periaortic node dissections were performed. Nodes were assayed for technetium-99m activity, and SLNs were established using the “10%-rule”, requiring that the radioactivity of additional SLNs be greater than 10% of the “hottest” SLN. Thirty-four nodal samples were assayed ex vivo for radioactivity. All the SLNs satisfying the “10%-rule” were detected using the FireFly system. Five fluorescent nodes were detected, corresponding with preoperative PET/CT scan. Three pigs had one SLN and one pig had two SLNs, with 100% concordance between fluorescence and radioactivity. Fluorescent-labeled Tilmanocept permits real-time intraoperative detection of SLNs during robotic-assisted lymphadenectomy for endometrial cancer in a porcine model. When radiolabeled with gallium-68, Tilmanocept allows for preoperative localization of SLNs using PET/CT, and shows specificity to SLNs with persistent fluorescent signal, detectable using the FireFly system, for two days post-injection. In conclusion, these findings suggest that a phase I trial in human subjects is warranted, and that a long-term goal of an intra-operative administration of non-radioactive fluorescent-labeled Tilmanocept is possible.

show abstract

Section: Introductionmentioning

confidence: 99%

Molecular Imaging of endometrial sentinel lymph nodes utilizing fluorescent-labeled Tilmanocept during robotic-assisted surgery in a porcine model

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…The resulting tri-modal agent provides high-resolution preoperative PET-images for SLN mapping and intraoperative localization of SLNs with both a conventional portable γ-probe and fluorescence imaging [ 108 ]. This tri-model agent has been successfully tested with reliable SLN identification in animal models [ 109 , 110 ]. Although PET lymphoscintigraphy using this tri-model agent might provide a solution to the issues addressed for SLNB in early-stage OSCC, it is indisputable that first it has to be investigated in a clinical trial with adequate reference standards.…”

Section: Discussionmentioning

confidence: 99%

New Developments in Imaging for Sentinel Lymph Node Biopsy in Early-Stage Oral Cavity Squamous Cell Carcinoma

Mahieu

Maar

Nieuwenhuis

et al. 2020

Cancers

View full text Add to dashboard Cite

Sentinel lymph node biopsy (SLNB) is a diagnostic staging procedure that aims to identify the first draining lymph node(s) from the primary tumor, the sentinel lymph nodes (SLN), as their histopathological status reflects the histopathological status of the rest of the nodal basin. The routine SLNB procedure consists of peritumoral injections with a technetium-99m [99mTc]-labelled radiotracer followed by lymphoscintigraphy and SPECT-CT imaging. Based on these imaging results, the identified SLNs are marked for surgical extirpation and are subjected to histopathological assessment. The routine SLNB procedure has proven to reliably stage the clinically negative neck in early-stage oral squamous cell carcinoma (OSCC). However, an infamous limitation arises in situations where SLNs are located in close vicinity of the tracer injection site. In these cases, the hotspot of the injection site can hide adjacent SLNs and hamper the discrimination between tracer injection site and SLNs (shine-through phenomenon). Therefore, technical developments are needed to bring the diagnostic accuracy of SLNB for early-stage OSCC to a higher level. This review evaluates novel SLNB imaging techniques for early-stage OSCC: MR lymphography, CT lymphography, PET lymphoscintigraphy and contrast-enhanced lymphosonography. Furthermore, their reported diagnostic accuracy is described and their relative merits, disadvantages and potential applications are outlined.

show abstract

“…Optical imaging such as multiphoton microscopy, fluorescence was used to study urinary bladder [18][19][20]. Computational model of urinary bladder was developed to study the propagation of ultraviolet and blue wavelength [21].…”

Section: Introductionmentioning

confidence: 99%

Hand‐held, clinical dual mode ultrasound ‐ photoacoustic imaging of rat urinary bladder and its applications

Sivasubramanian

Periyasamy

Dienzo

et al. 2018

Journal of Biophotonics

View full text Add to dashboard Cite

Urinary bladder imaging is critical to diagnose urinary tract disorders, and bladder cancer. There is a great need for safe, non-invasive, and sensitive imaging technique which enables bladder imaging. Photoacoustic imaging is a rapidly growing imaging technique for various biological applications. It can be combined with clinical ultrasound imaging system for hand-held, dual modal ultrasound-photoacoustic real-time imaging. Structural (bladder wall) and functional (accretion of nanoparticles) bladder imaging is shown here with combined ultrasound and photoacoustic imaging in rats. Photoacoustic images of bladder wall is shown using black ink as the contrast agent. Chicken tissues were stacked on the abdomen of the animal to demonstrate the feasibility of photoacoustic imaging till a depth of 2 cm. Also, the feasibility of photoacoustic imaging for a common bladder disorder, vesicoureteral reflux is studied using urinary tract mimicking phantom. It is also shown that a clinical ultrasound system can be used for photoacoustic imaging of non-invasive clearance study of gold nanorods from circulation by monitoring the gradual accumulation of the gold nanorods in the bladder. The time taken for accumulation of nanorods in the bladder can be used as an indicator of the clearance rate of the nanoparticle circulation from the body.

show abstract

Fluorescence-Based Molecular Imaging of Porcine Urinary Bladder Sentinel Lymph Nodes

Cited by 19 publications

References 35 publications

Molecular Imaging of endometrial sentinel lymph nodes utilizing fluorescent-labeled Tilmanocept during robotic-assisted surgery in a porcine model

Molecular Imaging of endometrial sentinel lymph nodes utilizing fluorescent-labeled Tilmanocept during robotic-assisted surgery in a porcine model

New Developments in Imaging for Sentinel Lymph Node Biopsy in Early-Stage Oral Cavity Squamous Cell Carcinoma

Hand‐held, clinical dual mode ultrasound ‐ photoacoustic imaging of rat urinary bladder and its applications

Contact Info

Product

Resources

About