Fluorescence behavior of 7-hydroxycoumarine excited by one-photon and two-photon absorption by means of a tunable dye laser

Parma, L.; Omenetto, N.

doi:10.1016/0009-2614(78)85281-6

Cited by 14 publications

(7 citation statements)

References 2 publications

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“…While some reports of a difference in emission spectra between single-and two-photon excitation have emerged, the mechanism has not been well understood. 46 For the fluorescence response observed in our experiments, two different photophysical pathways can be proposed. One is Förster resonance energy transfer (FRET) from two-photon excited amino acid residues, such as tryptophan, tyrosine, and phenylalanine.…”

Section: Discussionmentioning

confidence: 86%

Visible-wavelength two-photon excitation microscopy for fluorescent protein imaging

et al. 2015

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The simultaneous observation of multiple fluorescent proteins (FPs) by optical microscopy is revealing mechanisms by which proteins and organelles control a variety of cellular functions. Here we show the use of visible-light based two-photon excitation for simultaneously imaging multiple FPs. We demonstrated that multiple fluorescent targets can be concurrently excited by the absorption of two photons from the visible wavelength range and can be applied in multicolor fluorescence imaging. The technique also allows simultaneous single-photon excitation to offer simultaneous excitation of FPs across the entire range of visible wavelengths from a single excitation source. The calculation of point spread functions shows that the visible-wavelength two-photon excitation provides the fundamental improvement of spatial resolution compared to conventional confocal microscopy.

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Section: Discussionmentioning

confidence: 86%

Visible-wavelength two-photon excitation microscopy for fluorescent protein imaging

et al. 2015

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“…Most investigated fluorophores showed identical emission spectra irrespective of excitation wavelength (Xu and Webb 1996). However, on a few samples, a considerable red-shift of TPE emission spectra compared with SPE spectra (Bestvater et al 2002) and even variations in the shape of emission spectrum were found (Parma and Omenetto 1978).…”

Section: Resultsmentioning

confidence: 98%

Compact multiphoton/single photon laser scanning microscope for spectral imaging and fluorescence lifetime imaging

et al. 2004

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An inverted fluorescence microscope was upgraded into a compact three-dimensional laser scanning microscope (LSM) of 65 x 62 x 48 cm dimensions by means of a fast kHz galvoscanner unit, a piezodriven z-stage, and a picosecond (ps) 50 MHz laser diode at 405 nm. In addition, compact turn-key near infrared femtosecond lasers have been employed to perform multiphoton fluorescence and second harmonic generation (SHG) microscopy. To expand the features of the compact LSM, a time-correlated single photon counting unit as well as a Sagnac interferometer have been added to realize fluorescence lifetime imaging (FLIM) and spectral imaging. Using this unique five-dimensional microscope, TauMap, single-photon excited (SPE), and two-photon excited (TPE) cellular fluorescence as well as intratissue autofluorescence of water plant leaves have been investigated with submicron spatial resolution, <270 ps temporal resolution, and 10 nm spectral resolution.

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“…(55) However, there are cases where difference between one-and two-photon-excited fluorescence emission spectra were observed. (56) Figure 11.12 shows the emission spectra of fluorescein under 1PE and 2PE. For all the fluorophores described in this chapter, one-and twophoton-excited fluorescence emission spectra are identical.…”

Section: One- Two-and Three-photon-excited Fluorescence Emission Spementioning

confidence: 99%

Multiphoton Excitation of Molecular Fluorophores and Nonlinear Laser Microscopy

Webb

2002

Topics in Fluorescence Spectroscopy

102

112

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Molecular two-photon excitation (2PE) was first theoretically predicted byMaria Göppert-Mayer in 1931. (1) Like most other nonlinear processes, two-or three-photon excitation (3PE) requires high peak intensities, typically to photons for the observation of two-photon absorption. Months after the invention in 1960 of the pulsed ruby laser, it was used by Kaiser and Garrett to excite two-photon absorption in manifested by fluorescent deexcitation in the blue spectral region. (2) This was the second demonstration of nonlinear optics, closely following the demonstration of second-harmonic generation. Three-photon-excited fluorescence was observed in naphthalene crystals by Singh and Bradley in 1964, and the three-photon absorption cross section were estimated. (3)

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Fluorescence behavior of 7-hydroxycoumarine excited by one-photon and two-photon absorption by means of a tunable dye laser

Cited by 14 publications

References 2 publications

Visible-wavelength two-photon excitation microscopy for fluorescent protein imaging

Visible-wavelength two-photon excitation microscopy for fluorescent protein imaging

Compact multiphoton/single photon laser scanning microscope for spectral imaging and fluorescence lifetime imaging

Multiphoton Excitation of Molecular Fluorophores and Nonlinear Laser Microscopy

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