Fluorescence behavior of globular proteins from their bulk and thin film conformations in presence of mono-, di- and tri-valent ions

Bhowal, Ashim Chandra; Das, Kaushik; Kundu, Sarathi

doi:10.1016/j.colsurfb.2015.06.010

Cited by 12 publications

(5 citation statements)

References 29 publications

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“…As expected, the fluorescence intensity of VEGF-N,S-CDs increased at a higher VEGF concentration used for its conjugation to the N,S-CDs. 56,57 The enhancement in the fluorescence intensity of VEGF-N,S-CDs at a higher VEGF conjugation density occurred due to the stronger surface passivation of CDs and higher surface hydrophobicity 58 as a result of elimination of some nonradiative transitions 45,59,60 or suppressed quenching resulting from interactions with solvent or ions. A similar effect was observed for semiconductor and carbon quantum dots conjugated to proteins.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Carbon Dots Conjugated with Vascular Endothelial Growth Factor for Protein Tracking in Angiogenic Therapy

et al. 2020

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One of the challenges of using growth factors for tissue regeneration is to monitor their biodistributions and delivery to injured tissues for minimally invasive detection. In the present study, tracking of human vascular endothelial growth factor (VEGF) was achieved by chemically linking it to photoluminescent carbon dots (CDs). Carbon dots were synthesized by the hydrothermal method and, subsequently, conjugated with VEGF using carbodiimide coupling. ELISA and western blot analysis revealed that VEGF-conjugated CDs preserve the binding affinity of VEGF to its antibodies. We also show that VEGF-conjugated CDs maintain the functionality of VEGF for tube formation and cell migration. The VEGF-conjugated CDs were also used for in vitro imaging of human umbilical vein endothelial cells. The results of this work suggest that cell-penetrating VEGF-conjugated CDs can be used for growth factor protein tracking in therapeutic and tissue engineering applications.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Carbon Dots Conjugated with Vascular Endothelial Growth Factor for Protein Tracking in Angiogenic Therapy

et al. 2020

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“…To understand the possible nature of interactions via fluorescence quenching, S-V curves are plotted and the data are analyzed using the S-V equation [43,44] where, F 0 is the fluorescence intensity of the fluorophore in the absence of a quenching material, i.e. ions in our study, F is fluorescence intensity of the fluorophore in the presence of ions, M is the concentration of ions, k q is the quenching rate constant, τ 0 is the lifetime of fluorophore in the absence of a quencher and K sv is the S-V quenching constant.…”

Section: Fluorescence Quenching Studymentioning

confidence: 99%

Fluorescence emission and interaction mechanism of protein-coated gold and copper nanoclusters as ion sensors in different ionic environments

Bhowal

Pandit

Kundu

2018

J. Phys. D: Appl. Phys.

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Lysozyme protein-induced luminescent gold and copper nanoclusters (AuNCs and CuNCs) are prepared and their sensing capability is studied in the presence of different valent ions (Na + , Ca 2+ , Hg 2+ , Pb 2+ , Fe 3+ and La 3+ ions) in aqueous solutions of up to 100 µM salt concentration. Both types of nanocluster show good stability in aqueous conditions and, among all the chosen valent ions, it is found that maximum fluorescence quenching is observed in the presence of Hg 2+ and Pb 2+ ions for AuNCs and CuNCs, respectively. From steady-state fluorescence study and by using Stern-Volmer plots, the Stern-Volmer constant and other important thermodynamical parameters, i.e. changes in enthalphy, entropy and Gibbs free energy, are calculated. We propose a two step interaction process between the nanoclusters and the dissolved ions: first the interaction occurs between the protein-coated nanocluster surface and the ions, followed by the interaction of the metallic nanocluster core with the ions. Although thermodynamic investigation implies that both types of interaction are electrostatic in nature, selective fluorescence quenching of AuNCs and CuNCs in the presence of Hg 2+ and Pb 2+ ions occurs due to noncovalent metallophilic interactions between the cluster core and the dissolved ions.

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“…Globular protein exhibits uorescence property due to the presence of tryptophan and tyrosine amino acid residues. Protein-protein interaction can also be explained by studying the uorescence properties of the proteins [40,41]. In our previous work, we observed the enhancement of uorescence emission in the re-entrant region, and it was correlated with the hydrodynamic size as well as mobility of the protein [42].…”

Section: Introductionmentioning

confidence: 97%

Effect of Temperature on Re-entrant Condensation of Globular Protein in Presence of Tri-valent Ions

Pandit

Kundu

2022

J Fluoresc

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Globular proteins play several essential roles in functioning different mechanisms of the living organisms, and the stability of such protein molecules in an aqueous solution is strongly affected by multivalent ions. In this article, we have systematically studied the effect of temperature (between 5 and 25ºC) on the re-entrant condensation behaviour of bovine serum albumin (BSA) in the presence of trivalent ions, Yttrium (Y 3+ ), and Lanthanum (La 3+ ). The effect of temperature is explained considering the optical properties of the protein, i.e., from the optical absorption and emission behaviours. The absorption in the visible region and the uorescence emission of BSA becomes maximum at the lowest temperature. The decrement of mobility at lower temperature is responsible for uorescence enhancement. Moreover, the activation energy of the turbid or viscus phase of the BSA protein under reentrant condensation is enhanced in comparison with the transparent phase and the corresponding energy value is estimated from the uorescence study.

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Fluorescence behavior of globular proteins from their bulk and thin film conformations in presence of mono-, di- and tri-valent ions

Cited by 12 publications

References 29 publications

Carbon Dots Conjugated with Vascular Endothelial Growth Factor for Protein Tracking in Angiogenic Therapy

Carbon Dots Conjugated with Vascular Endothelial Growth Factor for Protein Tracking in Angiogenic Therapy

Fluorescence emission and interaction mechanism of protein-coated gold and copper nanoclusters as ion sensors in different ionic environments

Effect of Temperature on Re-entrant Condensation of Globular Protein in Presence of Tri-valent Ions

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