Fluorescence Behaviour of Crystallized C-Phycocyanin (Trimer) from Mastigocladus laminosus

Fluorescence Lifetimes in Photosynthetic Systems

“…This is born out also by a recent kinetic fluorescence study of phycocyanin crystals. The fluorescence decay and spectrum were largely altered with respect to the solution properties, probably due to strong exciton-coupling effects (Schneider et al, 1985b). It is to be expected that the structural data available from the study of Schirmer et al (1985) now provides the basis for a much more detailed interpretation of the kinetic energy transfer data than was possible so far.…”

“…This concept has essentially been confirmed now by direct observation of the timecourse of s-zf-energy transfer in a number of phycobiliproteins and also in some of the isolated protein subunits. At short emission wavelength, where the 's'-chromophores should preferentially emit, fast decay components with lifetimes in the range of 10-100 ps have been observed in several types of phycobiliproteins Wehrmeyer et al, 1985;Holzwarth et al, 1983;Schneider et al, 1985b;Hanzlik et al, 1985;Dagen et al, 1986). These fast decays turn into a risetime of equal time-constant when the fluorescence detection is shifted to longer wavelengths.…”