Fluorescence-coded DNA Nanostructure Probe System to Enable Discrimination of Tumor Heterogeneity via a Screening of Dual Intracellular microRNA Signatures in situ

Shin, Seung Won; Lee, Byoung Sang; Yang, Kisuk; Amornkitbamrung, Lunjakorn; Jang, Min; Ku, Bo Mi; Cho, Seung Woo; Lee, Jung Heon; Bae, Hojae; Oh, Byung Keun; Ahn, Myung Ju; Lim, Yong Taik; Um, Soong Ho

doi:10.1038/s41598-017-13456-3

Cited by 5 publications

(3 citation statements)

References 58 publications

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“…The end-overhangs vary greatly with their sequential designs, and they can be equipped with some functional modules through a chemical or enzymatic modification. It then enables to some practical applications involving a pathogenic biomolecular detection [13,[33][34][35]. Also, together with the end-to-end mutual binding of DNA building blocks in response to specific cue signals, new hydrogel forms composed of only DNAs have been produced [36,37].…”

Section: Structural Dna Nanotechnologymentioning

confidence: 99%

Hybrid material of structural DNA with inorganic compound: synthesis, applications, and perspective

et al. 2020

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Owing to its precise manipulation in nanoscale, DNA as a genetic code becomes a promising and generic material in lots of nanotechnological outstanding exploitations. The nanoscale assembly of nucleic acids in aqueous solution has showed very remarkable capability that is not achievable from any other material resources. In the meantime, their striking role played by effective intracellular interactions have been identified, making these more attractive for a variety of biological applications. Lately, a number of interesting attempts have been made to augment their marvelous diagnostic and therapeutic capabilities, as being integrated with inorganic compounds involving gold, iron oxide, quantum dot, upconversion, etc. It was profoundly studied how structural DNA-inorganic hybrid materials have complemented with each other in a synergistic way for better-graded biological performances. Such hybrid materials consisting of both structural DNAs and inorganics are gradually receiving much attention as a practical and futureoriented material substitute. However, any special review articles highlighting the significant and innovative materials have yet to be published. At the first time, we here demonstrate novel hybrid complexes made of structural DNAs and inorganics for some practical applications.

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Section: Structural Dna Nanotechnologymentioning

confidence: 99%

Hybrid material of structural DNA with inorganic compound: synthesis, applications, and perspective

et al. 2020

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“…Fluorescent dyes (e.g., fluorescein, cyanine dyes, and pyrene) have typically been used as fluorophores in nucleic acid probes because their properties vary according to the nature of the nucleic acid structure and the environment. ,,− For example, cyanine dyes having a wide emission range, from the visible to the infrared, can intercalate between base pairs, resulting in high-intensity fluorescence signals; pyrene units are usually applied in fluorescent turn-on and ratiometric detection systems, taking advantage of the sensitivity of their fluorescence signals in response to photoinduced electron transfer and excimer emission.…”

Section: Introductionmentioning

confidence: 99%

Pyrene-Modified Guanine Cluster Probes Forming DNA/RNA Hybrid Three-Way Junctions for Imaging of Intracellular MicroRNAs

Lee

Kim

2021

ACS Appl. Bio Mater.

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MicroRNAs (miRNAs) regulate gene expression in cells; high levels of expression are associated with various cancers. In this paper, we describe PyA-modified nucleic acid probes that can detect intracellular miRNAs by forming DNA/RNA hybrid three-way junction structures containing a fluorescent scaffolda so-called G-cluster. This G-cluster featured two mismatched strands, four guanine residues, and one fluorescent adenine residue having a pyrene moiety covalently connected at the 8-position through an acetylene linker. The scaffold underwent a dramatic shift in its emission wavelength when two mismatched strands formed a duplex, similar to the behavior of an adenine pentad system (A-cluster). We applied the G-cluster scaffold in a three-way junction system to probe for miRNAs; its red-shifted fluorescence intensity and stability were greater than those reported previously for A-cluster three-way junction probes. Furthermore, confocal microscopy of cancer cell lines revealed bright fluorescence emissions in response to the miRNAs in the cells. Thus, this system can be applied intracellularly as a potential fluorescent probe for the detection of various biologically important nucleic acids.

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“…A method for the fluorescence detection of the cellular mismatch repair ability in human cells is proposed to improve the detection of repair defects [4]. A fluorescence-coded DNA nanostructure probe system is presented to enable discrimination of tumor heterogeneity via a screening of dual intracellular microRNA signatures in situ [5].…”

Section: Introductionmentioning

confidence: 99%

A Low-Cost Time-Correlated Single Photon Counting Portable DNA Analyzer

Tian

Wei

2019

Sensors

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Photon-counting analysis of nucleic acids plays a key role in many diagnostics applications for its accurate and non-invasive nature. However, conventional photon-counting instrumentations are bulky and expensive due to the use of conventional optics and a lack of optimization of electronics. In this paper, we present a portable, low-cost time-correlated single photon-counting (TCSPC) analysis system for DNA detection. Both optical and electronic subsystems are carefully designed to provide effective emission filtering and size reduction, delivering good DNA detection and fluorescence lifetime extraction performance. DNA detection has been verified by fluorescence lifetime measurements of a V-carbazole conjugated fluorophore lifetime bioassay. The time-to-digital module of the proposed TCSPC system achieves a full width at half maximum (FWHM) timing resolution from 121 to 145 ps and a differential non-linearity (DNL) between −8.5% and +9.7% of the least significant bit (LSB) within the 500 ns full-scale range (FSR). With a detection limit of 6.25 nM and a dynamic range of 6.8 ns, the proposed TCSPC system demonstrates the enabling technology for rapid, point-of-care DNA diagnostics.

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Fluorescence-coded DNA Nanostructure Probe System to Enable Discrimination of Tumor Heterogeneity via a Screening of Dual Intracellular microRNA Signatures in situ

Cited by 5 publications

References 58 publications

Hybrid material of structural DNA with inorganic compound: synthesis, applications, and perspective

Hybrid material of structural DNA with inorganic compound: synthesis, applications, and perspective

Pyrene-Modified Guanine Cluster Probes Forming DNA/RNA Hybrid Three-Way Junctions for Imaging of Intracellular MicroRNAs

A Low-Cost Time-Correlated Single Photon Counting Portable DNA Analyzer

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