Fluorescence correlation spectroscopy and fluorescence lifetime imaging microscopy for deciphering the morphological evolution of supramolecular self-assembly

Kundu, Subhankar; Das, Subhadeep; Patra, Abhijit

doi:10.1039/d2cc06607f

Cited by 9 publications

(4 citation statements)

References 118 publications

(275 reference statements)

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“…Figure f shows the autocorrelation curve of the BSA–Cu NCs with its fitting analysis. Using Rhodamine B as a standard and with the help of the well-known Stokes–Einstein equation , for a diffusing particle model, the hydrodynamic diameter of Cu NCs was determined to be 5.7 ± 1 nm, which corroborates well the observed TEM images (Figure S2). The parameters obtained from the fitting analysis, namely, diffusion times ( τ D ), diffusion coefficients ( D t ), numbers of molecules ( N ), hydrodynamic radii ( r h ), and diameters of the BSA–Cu NCs, are presented in Table S1.…”

supporting

confidence: 80%

Unveiling the Long-Lived Emission of Copper Nanoclusters Embedded in a Protein Scaffold

Sharma,

Das,

Kaushik

et al. 2023

J. Phys. Chem. Lett.

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Protein-conjugated coinage metal nanoclusters have become promising materials for optoelectronics and biomedical applications. However, the origin of the photoluminescence, especially the long-lived excited state emission in these metal nanoclusters, is still elusive. Here, we unveiled the underlying mechanism of long-lived emission in albumin protein-conjugated copper nanoclusters (Cu NCs) using steady state and time-resolved spectroscopic techniques. Our findings reveal room-temperature phosphorescence (RTP) in protein-conjugated Cu NCs. Time-resolved area-normalized spectra distinguished short-and long-lived components, where the former arises from the singlet state and the latter from the triplet state, thus resulting in RTP. The similarity of the emission spectra at room (298 K) and cryogenic (77 K) temperature ascertains the RTP phenomenon by harvesting the higher-lying triplet states. Time-gated bioimaging of A549 cells using the long-lived emission not only supports RTP emission in the cellular environment but also provides exciting avenues in long-term bioimaging using bovine serum albumin−conjugated Cu NCs.

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supporting

confidence: 80%

Unveiling the Long-Lived Emission of Copper Nanoclusters Embedded in a Protein Scaffold

Sharma,

Das,

Kaushik

et al. 2023

J. Phys. Chem. Lett.

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“…On the other hand, fluorescence spectroscopic tools such as fluorescence correlation spectroscopy (FCS) and fluorescence lifetime imaging microscopy (FLIM) could emerge as new techniques to decipher the intriguing dynamic transformation. [79] In this context, fluorescent building blocks could be employed to understand the diffusion of parent entities as well as COF crystallites in the reaction medium. Scanning tunneling microscopy (STM)…”

Section: Discussionmentioning

confidence: 99%

Pushing the Boundaries of Covalent Organic Frameworks through Postsynthetic Linker Exchange

Shreeraj,

Giri,

Patra

2023

ChemNanoMat

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Covalent organic frameworks (COFs) are a fast‐developing family of porous organic materials that have received substantial research interest during the last two decades. Dynamic covalent chemistry (DCC) is the cornerstone of COF fabrication. DCC is a process that entails reversible bond breaking‐reforming under equilibrium to attain the thermodynamically most stable structure. Due to the reversible nature of the covalent linkages, the building blocks of pre‐synthesized COF or pre‐assembled chemical entities, like network polymers and supramolecular hosts, can be replaced postsynthetically under appropriate reaction conditions. The technique is known as postsynthetic linker exchange (PLE). PLE provides an easy way to introduce functional building blocks into the COF backbone. In this article, we have highlighted the recent advancements (from 2017 to 2023) in the postsynthetic linker exchange strategy for constructing highly crystalline and porous COFs that are often unattainable via de novo fabrication. The mechanistic insights of the linker exchange process for the transformation of various parent entities, such as COFs, amorphous covalent organic networks, linear polymers, and molecular cages to daughter COFs, have been deliberated with fascinating examples. We have also outlined some future avenues for applying the PLE process for the large‐scale fabrication of highly crystalline COFs for real‐time applications.

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Section: Fluorescence-guided Neurosurgerymentioning

confidence: 99%

“…New methods of FGS are now being studied that may permit more effective delineation of tumor tissue from the surrounding parenchyma [79]. Fluorescence lifetime imaging (FLIM) utilizes time-gated intensified cameras to visualize nicotinamide adenine dinucleotide (NADH, which is more highly expressed in tumor cells relative to normal brain tissue) and/or 5-ALA induced PpIX [79,80]. FLIM has showcased its potential to highlight areas of subtle 5-ALA fluorescence and to increase the ability to differentiate tumor cells from normal brain tissue when used to detect NADH in addition to PpIX [79].…”

Section: Fluorescence-guided Neurosurgerymentioning

confidence: 99%

Intraoperative Imaging and Optical Visualization Techniques for Brain Tumor Resection: A Narrative Review

Bin-Alamer,

Abou-Al-Shaar,

Gersey

et al. 2023

Cancers

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Advancements in intraoperative visualization and imaging techniques are increasingly central to the success and safety of brain tumor surgery, leading to transformative improvements in patient outcomes. This comprehensive review intricately describes the evolution of conventional and emerging technologies for intraoperative imaging, encompassing the surgical microscope, exoscope, Raman spectroscopy, confocal microscopy, fluorescence-guided surgery, intraoperative ultrasound, magnetic resonance imaging, and computed tomography. We detail how each of these imaging modalities contributes uniquely to the precision, safety, and efficacy of neurosurgical procedures. Despite their substantial benefits, these technologies share common challenges, including difficulties in image interpretation and steep learning curves. Looking forward, innovations in this field are poised to incorporate artificial intelligence, integrated multimodal imaging approaches, and augmented and virtual reality technologies. This rapidly evolving landscape represents fertile ground for future research and technological development, aiming to further elevate surgical precision, safety, and, most critically, patient outcomes in the management of brain tumors.

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Fluorescence correlation spectroscopy and fluorescence lifetime imaging microscopy for deciphering the morphological evolution of supramolecular self-assembly

Abstract: Properties and functions of noncovalent interactions-driven fluorescent supramolecular self-assembly greatly depend on their evolution dynamics. Electron microscopy, atomic force microscopy, and confocal laser scanning microscopy have been used to elucidate...

Cited by 9 publications

References 118 publications

Unveiling the Long-Lived Emission of Copper Nanoclusters Embedded in a Protein Scaffold

Unveiling the Long-Lived Emission of Copper Nanoclusters Embedded in a Protein Scaffold

Pushing the Boundaries of Covalent Organic Frameworks through Postsynthetic Linker Exchange

Intraoperative Imaging and Optical Visualization Techniques for Brain Tumor Resection: A Narrative Review

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