Fluorescence detection and determination of glyphosate via its N-nitroso derivative by thin-layer chromatography

Young, J. Christopher; Khan, Shahamat U.; Marriage, P. B.

doi:10.1021/jf60212a069

Cited by 30 publications

(18 citation statements)

References 19 publications

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“…Glyphosate was determined by the method of Young et al (20). From each of the triplicate seedling extracts 13.3 ml aliquots were pooled and dried under vacuum at 60 C. Lateral shoot extracts were dissolved in 3 ml and stem extracts in 4 ml of 0.12 N HC1.…”

Section: Methodsmentioning

confidence: 99%

Differential Varietal Tolerance of Peach(Prunus persica)Seedlings to Glyphosate

Marriage

Khan²

1978

Weed sci.

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The translocation and effects of glyphosate [N-(phosphonomethyl)glycine] were determined in greenhouse-grown peach [Prunus persica(L.) Batsch] seedlings obtained from different seed parents. Two lower branches of 10-week-old seedlings of Rutgers Red Leaf, Bailey, Kalamazoo, Siberian C, and Harrow Blood were sprayed with 0.9 to 18 mg of glyphosate per plant. Seedlings of Harrow Blood, Bailey, and Kalamazoo were more susceptible to injury than were those of Siberian C and Rutgers Red Leaf. Glyphosate at 6, 9, and 18 mg produced necrotic areas on the stems of the former seedlings and at the higher rates tree death resulted. The Siberian C and Rutgers Red Leaf seedlings showed only chlorosis and malformation of apical leaves although slight stem injury occurred 1 month after treatment. Measurements of plant weight, height, and stem diameter indicated that seedlings of Harrow Blood, Bailey, and Kalamazoo tolerated only 3 mg of glyphosate without growth reduction. This was one-half and one-third of the amount which seedlings of Siberian C and Rutgers Red Leaf tolerated, respectively. The concentration of glyphosate in the treated lower branches and the main stem of the seedlings was determined by thin-layer chromatography. The concentrations in the bark were generally similar among all seedlings. Differential tolerance, therefore, was not based on differences in overall glyphosate concentration in tissues but on protective or resistance mechanisms present only in seedlings of Siberian C and Rutgers Red Leaf.

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Section: Methodsmentioning

confidence: 99%

Differential Varietal Tolerance of Peach(Prunus persica)Seedlings to Glyphosate

Marriage

Khan²

1978

Weed sci.

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“…The formation of N-nitroso-compounds was measured by estimating the N-nitrosation of L-[ 14 C]-proline according to the method of Young et al (1977) by ascending thin layer chromatography with slight modification. AMs obtained from vitamin E supplemented animals were treated with LPS (1 and 10 Ag/ml) in the medium containing 15 mM cold proline and 1.0 ACi/ml [ 14 C]-proline for 48 hrs.…”

Section: Estimation Of N-nitrosoproline Formation By Amsmentioning

confidence: 99%

Inhibitory effect of vitamin E on proinflammatory cytokines-and endotoxin-induced nitric oxide release in alveolar macrophages

Khanduja

Avti

KUMARVANDANAPATHANIACHANDERMOHANP

2005

Life Sciences

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“…and not glycine is a photolysis product. Glyoxylic acid (5) standards could be detected at the 50 ng level using 0-aminodiphenyl spray technique (7) and at about 0.5 ug using the anilineimine technique (8). However, glyoxylic acid was not detected as a photolysis product of W-nitrosoglyphosate by either of these two methods.…”

Section: Formation and Persistence Of Af-nitrosoglyphosate In Soilmentioning

confidence: 99%

N-Nitrosamine Formation in Soil from the Herbicide Glyphosate and its Uptake by Plants

Khan

1981

ACS Symposium Series

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The herbicide glyphosate was nitrosated in aqueous solution by third order kinetics to N-nitrosoglyphosate. The nitrosation at 25°C was maximum at the reaction pH of 2.5 and had a pH-dependent rate constant of 2.4M -2 sec -1 . An activation energy of 9.5 Kcal mole -1 indicated that glyphosate in water is nitrosated very readily. Formation of N-nitrosoglyphosate was also observed when different soils were treated with sodium nitrite and the herbicide glyphosate at elevated levels. However, at low levels of glyphosate (5 ppm) and nitrite nitrogen (2 ppm) the formation of N-nitrosoglyphosate was not observed. The residues of N-nitrosoglyphosate can be assimilated by the roots of oat plants and translocated to the shoots. Formation of N-nitrosoglyphosate in soil and its uptake by plants under normal field conditions is not expected.In recent years greater attention has been given to nitrogen containing pesticides and the possibility of their nitrosation in soil. The W-nitrosamines that form may arise from the parent pesticide or from a pesticide metabolite. The reaction calls for favourable pH conditions (pH 3-4) and excess nitrite. Under field conditions, the nitrosable residues are usually present in traces and only small quantities of these will actually be nitrosated in soils. However, the possibility exists that the small amounts of W-nitrosamines could be assimilated by plants.Formation of W-nitroso compounds in soil from nitrite and nitrosable pesticides, such as s-triazine (1), dithiocarbamate and carbamate (2) and dinitroaniline (3) has been demonstrated under in vitro conditions. The herbicide glyphosate [W-(phosphonomethyl) glycine] (]) contains a secondary amine nitrogen, which can undergo W-nitrosation to form W-nitrosoglyphosate (2). Glyphosate has been widely used to provide effective control of most herbaceous perennial weeds through postemergence application (4). For this purpose the herbicide is applied to weed foliage at rates up to 4.2 kg/ha, often as a spot treatment.

show abstract

Fluorescence detection and determination of glyphosate via its N-nitroso derivative by thin-layer chromatography

Cited by 30 publications

References 19 publications

Differential Varietal Tolerance of Peach(Prunus persica)Seedlings to Glyphosate

Differential Varietal Tolerance of Peach(Prunus persica)Seedlings to Glyphosate

Inhibitory effect of vitamin E on proinflammatory cytokines-and endotoxin-induced nitric oxide release in alveolar macrophages

N-Nitrosamine Formation in Soil from the Herbicide Glyphosate and its Uptake by Plants

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