Fluorescence detection in high performance liquid chromatographic determination of polycyclic aromatic hydrocarbons

Das, Bejoy S.; Thomas, Glyn V.

doi:10.1021/ac50029a036

Cited by 71 publications

(19 citation statements)

References 14 publications

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“…Since chrysene normally elutes at Spectrum 108, the species represented in Figure 12B may be of a similar type as 9,10-dimethyl-1,2-benzanthracene. The occurrence of these large ring compound in fly ash is well known [18,19]. Our samples now appear to possess chemical .-_.…”

Section: Fluorescent Data Selectionmentioning

confidence: 55%

High performance liquid chromatography/video fluorometry. Part II: Applications

Shelly

Fogarty

Warner

1981

J. High Resol. Chromatogr.

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Section: Fluorescent Data Selectionmentioning

confidence: 55%

High performance liquid chromatography/video fluorometry. Part II: Applications

Shelly

Fogarty

Warner

1981

J. High Resol. Chromatogr.

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“…Application of similar excitation and emission conditions to the detection of PAH in HPLC has been reported earlier. 17 Calibration plots for the PAH were found to be linear at the concentrations studied which ranged from 1 to 250pg injected of each PAH in the mixture (D[ai]P 200-300 pg).…”

Section: H Plc-fluorescence Analysismentioning

confidence: 98%

Determination of Nanogram/Kilogram Levels of Polycyclic Aromatic Hydrocarbons in Foods by HPLC with Fluorescence Detection

Lawrence

Das²

1986

International Journal of Environmental Analytical Chemistry

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Methodology was developed for the determination of eight polycyclic aromatic hydrocarbons (PAH) in five food categories including meat/fish, dried dairy products, cereals, leafy vegetables and vegetable/marine oils. The eight PAH were fluoranthene, benzo[a]anthracene, benzo[k]fluoranthene, benzo[b]fluoranthene, benzo[a]pyrene, 7,12-dimethylbenzo[a]anthracene, dibenzo[ah]anthracene and dibenzo[ai]pyrene. Samples were digested with alcoholic KOH followed by partitioning into solvents such as cyclohexane or isooctane. Lipids and other interferences were removed by solvent partitioning with dimethylformamide or dimethylsulfoxide/water. Additional cleanup involving column chromatography on silica gel, Florisil or Sephadex LH-20 was employed as required. Reversed-phase chromatography with gradient elution and fluorescence detection was employed for the determinations. Confirmation was carried out by GC-MS/SIM. Detection limits ranged from 2-90 ng/kg depending on the PAH and food analyzed. Results of a small survey indicated that the meat/fish category had the highest levels (low microgram/kg, on average) of the foods studied.

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“…Samples were filtered and processed following the methods of Faust et al (2012); details may be found in Heintzman (2013). The use of GC-MS and HPLC for detection and quantification of PAHs is well-established based on exclusive absorbance and fluorescence characteristics (Das & Thomas, 1978;Law & Biscaya, 1994;DIONEX, 2009). In addition, GC-MS has previously been utilized for determination of PAHs in urban playa sediment samples (Faust et al, 2012).…”

Section: Water Sample Collection and Analysismentioning

confidence: 99%