Fluorescence detection of mercury(II) and lead(II) ions using aptamer/reporter conjugates

Lin, Yu‐Liang; Liu, Chi Wei; Chang, Huan‐Tsung

doi:10.1016/j.talanta.2011.01.016

Cited by 80 publications

(36 citation statements)

References 45 publications

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“…The ability to change fluorophores and the separation between the metal ion binding site and the fluorophore give a distinct advantage to DNAzyme-based fluorescent metal ion sensors. These fluorescence sensors typically have a limit of detection (LOD) in the low nanomolar to picomolar range and have been successfully applied to the detection of metal ion contaminants, including UO2 2+ , Pb 2+ , Hg 2+ , Cu 2+ , Ag + , Cr 3+ , and Ce 3+ , in tap [39,40], ground [18], pond [41,42], waste, river and lake water [23,43-45], as shown in Table 1. As a result, fluorescent sensors based on DNAzymes are commercially available and have been used to monitor drinking water systems in public schools [46].…”

Section: Fluorescent Metal Ion Sensors For Environmental Monitoring Amentioning

confidence: 99%

DNAzyme sensors for detection of metal ions in the environment and imaging them in living cells

McGhee

Loh

2017

Current Opinion in Biotechnology

128

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The on-site and real-time detection of metal ions is important for environmental monitoring and for understanding the impact of metal ions on human health. However, developing sensors selective for a wide range of metal ions that can work in the complex matrices of untreated samples and cells presents significant challenges. To meet these challenges, DNAzymes, an emerging class of metal ion-dependent enzymes selective for almost any metal ion, have been functionalized with fluorophores, nanoparticles and other imaging agents and incorporated into sensors for the detection of metal ions in environmental samples and for imaging the metal ions in living cells. Herein, we highlight the recent developments of DNAzyme-based fluorescent, colorimetric, SERS, electrochemical and electrochemiluminscent sensors for metal ions for these applications.

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Section: Fluorescent Metal Ion Sensors For Environmental Monitoring Amentioning

confidence: 99%

DNAzyme sensors for detection of metal ions in the environment and imaging them in living cells

McGhee

Loh

2017

Current Opinion in Biotechnology

128

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“…[153][154][155][156][157][158][159][160][161][162][163][164][165] Through monitoring the different emission of quantum dots, Freeman et al reported a uorescent sensor for multiplex detection of Hg 2+ and Ag + using nucleic acid-functionalized quantum dots. 161 The detection limit can reach 10 nM for Hg 2+ and 1 mM for Ag + .…”

Section: Simultaneous Detection Of Heavy Metal Ions (Pb 2+ Hg 2+ Anmentioning

confidence: 99%

Functional nucleic acid-based sensors for heavy metal ion assays

Zhu

Zhang

2014

Analyst

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Heavy metal contaminants such as lead ions (Pb(2+)), mercury ions (Hg(2+)) and silver ions (Ag(+)) can cause significant harm to humans and generate enduring bioaccumulation in ecological systems. Even though a variety of methods have been developed for Pb(2+), Hg(2+) and Ag(+) assays, most of them are usually laborious and time-consuming with poor sensitivity. Due to their unique advantages of excellent catalytic properties and high affinity for heavy metal ions, functional nucleic acids such as DNAzymes and aptamers show great promise in the development of novel sensors for heavy metal ion assays. In this review, we summarize the development of functional nucleic acid-based sensors for the detection of Pb(2+), Hg(2+) and Ag(+), and especially focus on two categories including the direct assay and the amplification-based assay. We highlight the emerging trends in the development of sensitive and selective sensors for heavy metal ion assays as well.

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“…Terbium has been used in a turn-on luminescent Tb-complex aptasensor for adenosine in undiluted and unprocessed serum [31], in an aptasensor to detect ochratoxin A in wheat [32,33], and to determine Hg 2þ and Pb 2þ [34]. The combination of gold nanoparticles and Tb-complexes both labelled to aptamers was successfully used to detect thrombin with LOD equal to 0.14 nM (~5 ng/ mL) [35].…”

Section: Introductionmentioning

confidence: 99%

A time-resolved luminescent competitive assay to detect L-selectin using aptamers as recognition elements

Cywiński

Olejko

Löhmannsröben

2015

Analytica Chimica Acta

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Fluorescence detection of mercury(II) and lead(II) ions using aptamer/reporter conjugates

Cited by 80 publications

References 45 publications

DNAzyme sensors for detection of metal ions in the environment and imaging them in living cells

DNAzyme sensors for detection of metal ions in the environment and imaging them in living cells

Functional nucleic acid-based sensors for heavy metal ion assays

A time-resolved luminescent competitive assay to detect L-selectin using aptamers as recognition elements

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