Fluorescence detection to determine proteins and humic-like substances fingerprints of exopolymeric substances (EPS) from biological sludges performed by size exclusion chromatography (SEC)

Bhatia, Divya; Bourven, Isabelle; Simon, Stéphane; Bordas, François; Hullebusch, Eric D. van; Rossano, Stéphanie; Lens, Piet N.L.; Guibaud, Gilles

doi:10.1016/j.biortech.2012.12.078

Cited by 68 publications

(25 citation statements)

References 31 publications

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“…Lyko et al (2007) suggested that three main molar mass fractions can be distinguished: high MW organic compounds (>5000 Da) mainly composed of proteins and polysaccharides, mid-MW compounds (1000e5000 Da) and organic matter of low MW (<1000 Da). Bhatia et al (2013) found that the MW of protein-like substances in EPS samples extracted from activated sludge was in a wide range of 1e600 kDa. The impact of CaO 2 dosage and reaction time on MW distributions of SEPS fractions was presented in Fig.…”

Section: Molecular Weight Distribution Analysismentioning

confidence: 99%

Enhancement of waste activated sludge dewaterability using calcium peroxide pre-oxidation and chemical re-flocculation

et al. 2016

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a b s t r a c tThe effects of combined calcium peroxide (CaO 2 ) oxidation with chemical re-flocculation on dewatering performance and physicochemical properties of waste activated sludge was investigated in this study. The evolutions of extracellular polymeric substances (EPS) distribution, composition and morphological properties were analyzed to unravel the sludge conditioning mechanism. It was found that sludge filtration performance was enhanced by calcium peroxide oxidation with the optimal dosage of 20 mg/ gTSS. However, this enhancement was not observed at lower dosages due to the absence of oxidation and the performance deteriorated at higher dosages because of the release of excess EPS, mainly as proteinlike substances. The variation in soluble EPS (SEPS) component can be fitted well with pseudo-zero-order kinetic model under CaO 2 treatment. At the same time, extractable EPS content (SEPS and loosely bound EPS (LB-EPS)) were dramatically increased, indicating sludge flocs were effectively broken and their structure became looser after CaO 2 addition. The sludge floc structure was reconstructed and sludge dewaterability was significantly enhanced using chemical re-flocculation (polyaluminium chloride (PACl), ferric iron (FeCl 3 ) and polyacrylamide (PAM)). The inorganic coagulants performed better in improving sludge filtration dewatering performance and reducing cake moisture content than organic polymer, since they could act as skeleton builders and decrease the sludge compressibility.

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Section: Molecular Weight Distribution Analysismentioning

confidence: 99%

Enhancement of waste activated sludge dewaterability using calcium peroxide pre-oxidation and chemical re-flocculation

et al. 2016

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“…Protein composition in the crude EPS was believed to enhance the mechanism of bioflocculation. EPS was produced by microorganisms for various purposes in reaction to environmental stresses (Bhatia et al, 2013). Most of bioflocculants by microorganisms were formed during their growth phase.…”

Section: Discussionmentioning

confidence: 99%

Description of novel marine bioflocculant-producing bacteria isolated from biofloc of Pacific whiteleg shrimp,Litopenaeus vannameiculture ponds

et al. 2018

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19Description of marine bioflocculant-producing bacteria isolated from biofloc of 20 Pacific whiteleg shrimp, Litopenaeus vannamei culture ponds was prompted to 21 explore the bacteria that enhanced bioflocculation process in aquaculture wastewater 22 treatment. Certain marine bacteria were potentially secreted extracellular polymeric 23 substances (EPS) which response to the physiological stress encountered in the natural 24 environment that can act as bioflocculants. This study aimed to identify marine 25 2 bioflocculant-producing bacteria isolated from biofloc; to evaluate their flocculating 26 activities; and to characterize their protein in EPS. Phenotypic and genotypic 27 identification of the bacteria including morphological and molecular approaches were 28 employed, while their flocculating activities were examined via Kaolin clay 29 suspension method and statistically analyzed. The EPS that acted as bioflocculants 30 were extracted using cold ethanol precipitation method. Protein concentration was 31 determined by Bradford assay and protein profiling was finally completed with 32 Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) method. 33 Six species of marine bacteria known as Halomonas venusta, Bacillus cereus, Bacillus 34 subtilis, Bacillus pumilus, Nitratireductor aquimarinus and Pseudoalteromonas sp. 35 were successfully identified as bioflocculant-producing bacteria. The highest 36 flocculating activity was exhibited by Bacillus cereus at 93%, while Halomonas 37venusta showed the lowest record at 59%. All bioflocculant-producing bacteria 38 species showed different protein concentration that ranged between 1.377 µg/mL to 39 1.455 µg/mL. Several protein bands with different molecular weight that ranged 40 between 16 kDa to 100 kDa were observed. This study revealed that all the identified 41 bacteria species have high potential characteristics to initiate aquaculture wastewater 42 treatment and may play important roles in bioflocculation process. 43 Importance: 46 Six species of marine bacteria isolated from biofloc of Pacific whiteleg shrimp, 47 Litopenaeus vannamei culture ponds were identified as bioflocculant-producing 48 bacteria. Among those six species, Bacillus cereus, Bacillus pumilus, Nitratireductor 49 3 aquimarinus and Pseudoalteromonas sp. were highly potential to be used as booster 50 for rapid formation of biofloc due to their high flocculating activities. Protein content 51 in EPS of novel marine biofocculant-producing bacteria has beneficial consequences 52 on degradation process of organic substances, denitrification of wastes and ions 53 elimination from aquaculture wastes.54

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“…The component information of EPS extracts is usually determined by Fourier transform-infrared (FT-IR) spectroscopy, three-dimensional excitation-emission matrix (3D-EEM) fluorescence spectroscopy, X-ray photoelectron spectroscopy, and so on (Badireddy et al, 2010;Bhatia et al, 2013;Sheng et al, 2013). In recent years, Raman spectroscopy has actually been used to characterize the EPS (Wagner et al, 2009;Chen et al, 2013b).…”

Section: Introductionmentioning

confidence: 99%

Composition of EPS fractions from suspended sludge and biofilm and their roles in microbial cell aggregation

et al. 2014

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Fluorescence detection to determine proteins and humic-like substances fingerprints of exopolymeric substances (EPS) from biological sludges performed by size exclusion chromatography (SEC)

Cited by 68 publications

References 31 publications

Enhancement of waste activated sludge dewaterability using calcium peroxide pre-oxidation and chemical re-flocculation

Enhancement of waste activated sludge dewaterability using calcium peroxide pre-oxidation and chemical re-flocculation

Description of novel marine bioflocculant-producing bacteria isolated from biofloc of Pacific whiteleg shrimp,Litopenaeus vannameiculture ponds

Composition of EPS fractions from suspended sludge and biofilm and their roles in microbial cell aggregation

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