Fluorescence determination of streptomycin in serum by reversed-phase ion-pairing liquid chromatography

Kubo, Hiroaki; Kobayashi, Yoshie; Kinoshita, Toshio

doi:10.1021/ac00126a017

Cited by 22 publications

(14 citation statements)

References 11 publications

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“…Streptomycin has no important chromophore group and is generally derivatized in order to obtain ultraviolet (UV)-absorbing or fluorescent compounds (Kurosawa et al, 1985;Kubo et al, 1986;Gerhardt et al, 1994).…”

Section: Methods Performancementioning

confidence: 99%

“…Reaction conditions for post-column derivatization were studied by Kubo et al (1986), who reported maximum sensitivity for DST at a reagent concentration of 0.5 M NaOH, a reaction temperature of 65°C and a reaction coil length of 15 m. A temperature of 70°C and a reaction coil length of 10 m were chosen in the present work because they produced a better signal-to-noise ratio for the analysis of sample extracts. The NaOH concentration was lowered from 0.5 to 0.1 M after repeated problems with detector cell leakage.…”

Section: Methods Performancementioning

confidence: 99%

“…Since streptomycin and dihydrostreptomycin do not possess a strong UV-absorbing chromophore group, the state-of-the-art analytical method for their determination at trace levels is liquid chromatography (LC) with post-column derivatization and fluorescence detection (Kubo et al, 1986). The complexity of food samples makes it necessary to perform sample clean-up by solid-phase extraction (SPE) prior to LC-fluorescence analysis (Gerhardt et al, 1994;Abbasi and Hellenäs, 1998).…”

Section: Introductionmentioning

confidence: 99%

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Monitoring of streptomycin and dihydrostreptomycin residual levels in porcine meat press juice and muscle via solid‐phase fluorescence immunoassay and confirmatory analysis by liquid chromatography after post‐column derivatization

Pyun

El‐Aty

Hashim

et al. 2007

Biomedical Chromatography

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A solid-phase fluorescence immunoassay (SPFIA) that was primarily developed for detection of antibiotic residues in milk was qualitatively applied for the pre-screening of the residues of aminoglycoside antibiotics, streptomycin and dihydrostreptomycin, in meat press juice. The confirmation of both analytes was performed using a validated method of highperformance liquid chromatography with post-column derivatization. The analytical performance was demonstrated by the analysis of pork meat samples spiked at three concentration levels, ranging from 0.25 to 2.5 ppm for each analyte. In general, the recoveries ranged from 80.4 to 81.5% and from 79.6 to 84.4% for streptomycin and dihydrostreptomycin, respectively, with relative standard deviations lower than 6%. The limits of detection were 0.1 and 0.15 ppm for streptomycin and dihydrostreptomycin, respectively, and the limits of quantification of 0.35 and 0.5 ppm are below the maximum residue limits of Codex, the European Union, and the Korean Food and Drug Administration (ranging from 0.5 to 0.6 ppm). Eight real samples collected from the Seoul area were first monitored using SPFIA, and none of them were found positive. These findings are in good accordance with those observed by HPLC analysis. To the best of our knowledge, this is the first report to monitor the aminoglycoside residues in pork meat press juice using SPFIA.

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Section: Methods Performancementioning

confidence: 99%

Section: Methods Performancementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Monitoring of streptomycin and dihydrostreptomycin residual levels in porcine meat press juice and muscle via solid‐phase fluorescence immunoassay and confirmatory analysis by liquid chromatography after post‐column derivatization

Pyun

El‐Aty

Hashim

et al. 2007

Biomedical Chromatography

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“…(181,182) Fluorescent detection of streptomycin reacted through the guanidino groups with naphthoquinone-4-sulfonate in alkaline solution has been reported in serum samples. (183) Photothermal derivatization of ciprofloxacin and its metabolites permits fluorescent detection throughout a linear range of about 2-1000 ng mL −1 . Photolysis with electrochemical detection of penicillins and cefoperazone has provided detection limits of about 6 ng.…”

Section: Antibioticsmentioning

confidence: 99%

Chemical Reagents and Derivatization Procedures in Drug Analysis

Danielson

Gallagher

Bao

2008

Encyclopedia of Analytical Chemistry

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This article describes both pre‐ and postcolumn derivatization chemistry used in conjunction with either chromatography or capillary electrophoresis (CE) to facilitate the determination of drugs. Generally, only prederivatization is used in gas chromatography (GC), principally to enhance the volatility, temperature stability, and/or detectability. The GC section considers derivatization of drugs by reagent class: alkylation, acylation, and silylation. The GC sample‐handling section describes an approach that combines the extraction and derivatization steps together. Both pre‐ and postcolumn derivatization are common approaches for high‐performance liquid chromatography (HPLC) and many of these methods have been adapted for CE. Derivatization for HPLC is often directed toward aliphatic amines, carboxylic acids, or alcohols that are difficult to detect at low levels by absorbance, luminescence, or electrochemical means. In addition, small hydrophilic molecules upon prederivatization are often converted into larger more hydrophobic compounds, making reversed‐phase HPLC easier or even feasible. The HPLC section considers derivatization of drugs based on the following types: alkaloids, amines, antibiotics, barbiturates, carbonyl compounds and carboxylic acids, catecholamines, hydroxy compounds, steroids, and sulfur compounds. For the GC and HPLC sections, tables are included, giving the structures of the more important derivatizing agents, the analytes, and the corresponding reaction products. A brief rationale for derivatization chemistry with CE concludes this article. For CE, derivatization is often done to improve detectability as the path length for absorbance detection is very short and fluorescence can be effective using laser‐based systems.

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“…Streptomycin was the first aminoglycoside antibiotic to be used in chemotherapy (Kurosawa et al, 1985;Kubo et al, 1986;Kubo et al, 1987).…”

Section: Streptomycinmentioning

confidence: 99%

Aminoglycoside antibiotics — two decades of their hplc bioanalysis

Šoltés

1999

Biomed. Chromatogr.

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Fluorescence determination of streptomycin in serum by reversed-phase ion-pairing liquid chromatography

Cited by 22 publications

References 11 publications

Monitoring of streptomycin and dihydrostreptomycin residual levels in porcine meat press juice and muscle via solid‐phase fluorescence immunoassay and confirmatory analysis by liquid chromatography after post‐column derivatization

Monitoring of streptomycin and dihydrostreptomycin residual levels in porcine meat press juice and muscle via solid‐phase fluorescence immunoassay and confirmatory analysis by liquid chromatography after post‐column derivatization

Chemical Reagents and Derivatization Procedures in Drug Analysis

Aminoglycoside antibiotics — two decades of their hplc bioanalysis

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