Fluorescence digital image analysis of the inositol trisphosphate‐mediated calcium transient in single permeabilized parietal cells

Tsunoda, Yasuhiro; Yodozawa, Susumu; Tashiro, Yutaka

doi:10.1016/0014-5793(88)80696-3

Cited by 15 publications

(4 citation statements)

References 16 publications

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“…Our results are consistent with the existence of at least two distinct Ca2+ stores in parietal cells, since caffeine can further release Ca2+ in permeabilized cells even after depletion of Ca2+ stores provoked by a maximal concentration of Ins(1,4,5)P3. These results are in agreement with a previous report [41], which showed that Ins(1,4,5)P3 can partially release Ca2+ from intracellular Ca2+ stores in permeabilized parietal cells. Moreover, Tsunoda et al [43] demonstrated that in parietal cells gastrin can induce Ca2+ waves propagating within the cytosol, consistent with the existence of two different Ca2+ stores in this cell type.…”

Section: Gastrin Receptor and Muscarinic Receptor Regulate Common Ca2supporting

confidence: 94%

Receptor-operated Ca2+ channels in gastric parietal cells: gastrin and carbachol induce Ca2+ influx in depleting intracellular Ca2+ stores

Roche

Balì

Magous

1993

Biochemical Journal

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The mechanism whereby gastrin-type receptor and muscarinic M3-type receptor regulate free intracellular Ca2+ concentration ([Ca2+]i) was studied in rabbit gastric parietal cells stimulated by either gastrin or carbachol. Both agonists induced a biphasic [Ca2+]i response: a transient [Ca2+]i rise, followed by a sustained steady state depending on extracellular Ca2+. Gastrin and carbachol also caused a rapid and transient increase in Mn2+ influx (a tracer for bivalent-cation entry). Pre-stimulation of cells with one agonist drastically decreased both [Ca2+]i increase and Mn2+ influx induced by the other. Neither diltiazem nor pertussistoxin treatment had any effect on agonist-stimulated Mn2+ entry. Thapsigargin, a Ca(2+)-pump inhibitor, induced a biphasic [Ca2+]i increase, and enhanced the rate of Mn2+ entry. Preincubation of cells with thapsigargin inhibits the [Ca2+]i increase as well as Mn2+ entry stimulated by gastrin or by carbachol. Thapsigargin induced a weak but significant increase in Ins(1,4,5)P3 content, but this agent had no effect on the agonist-evoked Ins(1,4,5)P3 response. In permeabilized parietal cells, Ins(1,4,5)P3 and caffeine caused an immediate Ca2+ release from intracellular pools, followed by a reloading of Ca2+ pools which can be prevented in the presence of thapsigargin. We conclude that (i) gastrin and carbachol mobilize common Ca2+ intracellular stores, (ii) Ca2+ permeability secondary to receptor activation involves neither a voltage-sensitive Ca2+ channel nor a GTP-binding protein from the G1 family, and (iii) agonists regulate common Ca2+ channels in depleting intracellular Ca2+ stores.

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Section: Gastrin Receptor and Muscarinic Receptor Regulate Common Ca2supporting

confidence: 94%

Receptor-operated Ca2+ channels in gastric parietal cells: gastrin and carbachol induce Ca2+ influx in depleting intracellular Ca2+ stores

Roche

Balì

Magous

1993

Biochemical Journal

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“…It seems likely that this rapid increase in Ins(I,4,S)Pj content may bc related to the already described [r-lo] peak increase in intracellular (Ca'* ] induced by gascrin: (i) the time course of Ins (l,4,5)P~ accumulation fits in well with that of intracellular (Ca2+ ] increase, (ii) both events are independent on extracellular Ca2 * [9] and (iii) Ins(l,4,5)P3 was shown to induce a rapid rise in intracellular [Ca'"] in pcrmeabilized gastric parietal cells [33,34].…”

Section: I~~(l45)~3mentioning

confidence: 98%

Biphasic kinetics of inositol 1,4,5‐trisphosphate accumulation, in gastrin‐stimulated parietal cells Effects of pertussis toxin and extracellular calcium

et al. 1991

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The effect of Pertussis toxin (PTx) and extracellular Ca2+ were investigated on gastrin‐induced Ins(1,4,5)P3 mass level in isolated gastric parietal cells. Basal Ins(1,4,5)P3 content was 5.48±0.49 pmol/500 000 cells. Gastrin (10 nM) induced a rapid increase in Ins(1,4,5)P3 content which was maximal after 15 s and corresponded to 2–2.5‐fold basal level; this Ins(1,4,5)P3 content then decreased within 30 s. After a longer time of gastrin exposure (> 1 min), a sustained and unexpected increased in Ins(1,4,5)P3 accumulation was observed which was maximal at 7.5 min (corresponding to 2.3–2.8‐fold basal value) and slightly decreased thereafter. PTx treatment of cells (200 ng/ml) for 3 h or removal extracellular Ca2+ did not affect the rapid rise, but drastically reduced the sustained increase in Ins(1,4,5)P3 content (60–100% inhibition); this inhibition was not evident after 10 min of hormone stimulation. Furthermore, diltiazem, a Ca2+ channel blocker, led to a similar inhibition of the sustained increase. We concluded that: (i) gastrin induced rapid increase in Ins(1,4,5)P3 content via a mechanism insensitive to PTx and to extracellular Ca2+, and (ii) gastrin induced a sustained increase in Ins(1,4,5)P3 level via a mechanism sensitive to PTx and to extracellular Ca2+. Even though the rapid rise in Ins(1,4,5)P3 content may be involved in the intracellular Ca2+ mobilization occurring after the first seconds of hormone stimulation, the physiological role of the sustained Ins(1,4,5)P3 increased level remains to be elucidated.

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“…The curve can be established in vitro (i.e. using solutions of the stain in a series of buffers at different pH) or in vivo (using labeled For calcium the in vitro calibration approach proposed by Grynkiewiez et al [195] is generally adopted [197][198]:…”

Section: ) Pellet Convex Area -Core Convex Areamentioning

confidence: 99%

Biomass Quantification by Image Analysis

Pons

Vivier

1999

Bioanalysis and Biosensors for Bioprocess Monitoring

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Microbiologists have always rely on microscopy to examine microorganisms. When microscopy, either optical or electron-based, is coupled to quantitative image analysis, the spectrum of potential applications is widened: counting, sizing, shape characterization, physiology assessment, analysis of visual texture, motility studies are now easily available for obtaining information on biomass. In this chapter the main tools used for cell visualization as well as the basic steps of image treatment are presented. General shape descriptors can be used to characterize the cell morphology, but special descriptors have been defined for filamentous microorganisms. Physiology assessment is often based on the use of fluorescent dyes. The quantitative analysis of visual texture is still limited in bioengineering but the characterization of the surface of microbial colonies may open new prospects, especially for cultures on solid substrates. In many occasions, the number of parameters extracted from images is so large that data-mining tools, such as Principal Components Analysis, are useful for summarizing the key pieces of information.

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Fluorescence digital image analysis of the inositol trisphosphate‐mediated calcium transient in single permeabilized parietal cells

Abstract: The myo-inositol 1,4,5trisphosphate (IPs)-induced Caz + mobilization in single saponin-permeabilixed and fura-2-loaded

Cited by 15 publications

References 16 publications

Receptor-operated Ca2+ channels in gastric parietal cells: gastrin and carbachol induce Ca2+ influx in depleting intracellular Ca2+ stores

Receptor-operated Ca2+ channels in gastric parietal cells: gastrin and carbachol induce Ca2+ influx in depleting intracellular Ca2+ stores

Biphasic kinetics of inositol 1,4,5‐trisphosphate accumulation, in gastrin‐stimulated parietal cells Effects of pertussis toxin and extracellular calcium

Biomass Quantification by Image Analysis

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