Fluorescence emission and fluorescence quenching as detection methods in isotachophoresis

Abstract

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Determination of B2 and B6 vitamers in serum by capillary electrophoresis-molecular fluorescence-charge coupled detector

“…To overcome the lack of sensitivity, preconcentration steps are often included to offset the low levels of vitamins in the samples. Concerning detection, the best results are obtained when fluorescence detectors are employed [5,22,23]. However, the different chemical structures make the development of analysis methods difficult for two or more B vitamin groups as their emission occurs at different wavelengths.…”

Comparison of column-coupled electrophoresis with liquid chromatography methods in food analysis of quinine

“…(U.S. Department of Health and Human Services, 2010;Lester, 1994). High performance separation techniques such a HPLC and CE (CZE, MEKC, CITP) and a variety of detection systems (UV, DAD, LIF, MS) have been reported in the literature for the analysis of QUI in model as well as real (beverages, pharmaceuticals, urine, plasma) matrices (Altria & Simpson, 1988;Buchberger et al, 2010;Feas et al, 2009;Hodel et al, 2009;Horie et al, 2006;Ibrahim et al, 2007;Junior et al, 2009;Marini et al, 2010;Quing & Jing, 2001;Reijenga et al, 1985;Samanidou et al, 2004;Steiner & Hassel, 2005;Tsimachidis et al, 2008;Zaugg & Thorman, 2001;Zhang et al 2007). From among those, the methods in Table 1 have been used for the analysis of QUI in beverages.…”

Indirect Detection of Halide Ions via Fluorescence Quenching of Quinine Sulfate in Microcolumn Ion Chromatography

“…[1][2][3][4][5][6][7][8][9][10][11] The quenching of fluorescence was typically used for metal cations, but the ability of halide ions to quench fluorescence has also been reported to involve quinine sulfate 1,2 and other dyes. [3][4][5][6][7] Fluorescence quenching has been utilized for the detection of non-fluorescent species in various separation methods.…”

“…The negative signal was primarily the result of quenching of the PNA by the analyte in the micelle's core. Reijenga et al 10 presented an apparatus for simultaneous fluorescence and UV absorption detection in isotachophoresis. They demonstrated fluorescence quenching as a method of identification for non-fluorescent compounds, using quinine as a fluorescent counter ion.…”

“…[3][4][5][6][7] Fluorescence quenching has been utilized for the detection of non-fluorescent species in various separation methods. [8][9][10][11] Amankwa and Kuhr 8 accomplished indirect fluorimetric detection using quinine sulfate as the visualization agent in micellar electrokinetic chromatography. They found that the detection mechanism involved a combination of displacement of the fluorophore from the micelle by the analytes and net reduction of the quantum efficiency of the fluorophore in the sample zone.…”