Fluorescence‐Emission Control of Single CdSe Nanocrystals Using Gold‐Modified AFM Tips

Eckel, Rainer; Walhorn, Volker; Pelargus, Christoph; Martini, Joerg; Enderlein, Jörg; Nann, Thomas; Anselmetti, Dario; Ros, Robert

doi:10.1002/smll.200600130

Cited by 27 publications

(35 citation statements)

References 43 publications

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“…It has been shown that quenching can be utilized to probe distances in the nanometer or sub-nanometer range (Berndt et al, 2010;Marme et al, 2003;Selvin, 2000). The impact of metal tips or nanostructures close to fluorophores has been well characterized (Ambrose et al, 1994;Anger et al, 2006;Ebenstein et al, 2006;Eckel et al, 2007;Krug et al, 2005;Kuehn et al, 2006;Xie & Dunn, 1994;Yoskovitz et al, 2011). Here, we exploit the quenching of organic fluorophores by sharp ndoped silicon tips to record topographical and optical images simultaneously with nanometer resolution.…”

Section: Introductionmentioning

confidence: 99%

Tip induced fluorescence quenching for nanometer optical and topographical resolution

et al. 2013

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Progress in nanosciences and life sciences is closely related to developments of high resolution imaging techniques. We introduce a technique which produces correlated topography and fluorescence lifetime images with nanometer resolution. Spot sizes below 5 nm are achieved by quenching of the fluorescence with silicon probes of an atomic force microscope which is combined and synchronized with a confocal fluorescence lifetime microscope. Moreover, we demonstrate the ability to locate and resolve the position of two fluorescent molecules separated by 20.7 nm on a DNA origami triangle with 120 nm side length by correlating topography and fluorescence data. With this method, we anticipate applications in nano-and life sciences, such as the determination of the structure of macromolecular assemblies on surfaces, molecular interactions, as well as the structure and function of nanomaterials.

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Section: Introductionmentioning

confidence: 99%

Tip induced fluorescence quenching for nanometer optical and topographical resolution

et al. 2013

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“…[26][27][28][29][30][31][32] Experiments on model systems formed by single dipole emitters such as molecules and semiconductor nanocrystals and spherical metal particles revealed a distance dependent interplay between competing enhancement and quenching processes. [33][34][35][36][37][38][39] Here the role of emitter dipole orientation for the interplay between quenching and fluorescence enhancement becomes apparent. 40 Small cone angles in tip-shaped metal structure are predicted to reduce energy dissipation as compared to spherical particles.…”

Section: Tip-enhanced Fluorescence-mentioning

confidence: 99%

Tip‐Enhanced Near‐Field Optical Microscopy

Hartschuh¹

2014

Handbook of Spectroscopy

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Tip-enhanced near-field optical microscopy (TENOM) is a scanning probe technique capable of providing a broad range of spectroscopic information on single objects and structured surfaces at nanometer spatial resolution and with highest detection sensitivity. In this review, we first illustrate the physical principle of TENOM that utilizes the antenna function of a sharp probe to efficiently couple light to excitations on nanometer length scales. We then discuss the antennainduced enhancement of different optical sample responses including Raman scattering, fluorescence, generation of photocurrent and electroluminescence. Different experimental realizations are presented and several recent examples that demonstrate the capabilities of the technique are reviewed.

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“…It has been shown that the combination of AFM and fluorescence microscopy offers new opportunities to gather multidimensional data from a given system [31][32][33][34][35][36] , but it can also make use of fluorescence modulation effects which arise from the presence of the sharp AFM probe in the vicinity of fluorescent molecules. These effects include the enhancement [11][12][13]16 and quenching 17,18,20,22,23,37,38 of the fluorescence and a change in the fluorescence lifetime 23 . Since both enhancement and quenching are highly localized phenomena, they can lead to much higher resolution than the diffraction limit dictates for optical imaging.…”

Section: Introductionmentioning

confidence: 98%

“…The basis for all high resolution near field applications is the emission behavior of fluorophores on surfaces in close proximity to a sharp tip. This behavior has been studied experimentally as well as theoretically [10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29] . The fluorescence properties of fluorophores (e.g.…”

Section: Introductionmentioning

confidence: 99%

“…In bulk experiments, silicon has been shown to quench the fluorescence of a layer of organic dyes that is separated from a silicon wafer using Langmuir-Blodgett films 43 . Several studies have investigated the quenching properties of metal coated tips [17][18][19][20]22,23 and silicon tips 22,23 . So far though, metal tips have been proposed to yield better optical resolutions in combined AFM/fluorescence quenching experiments than silicon tips.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous single molecule atomic force and fluorescence lifetime imaging

Schulz

Koberling

Walters³

et al. 2010

Single Molecule Spectroscopy and Imaging III

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The combination of atomic force microscopy (AFM) with single-molecule-sensitive confocal fluorescence microscopy enables a fascinating investigation into the structure, dynamics and interactions of single biomolecules or their assemblies. AFM reveals the structure of macromolecular complexes with nanometer resolution, while fluorescence can facilitate the identification of their constituent parts. In addition, nanophotonic effects, such as fluorescence quenching or enhancement due to the AFM tip, can be used to increase the optical resolution beyond the diffraction limit, thus enabling the identification of different fluorescence labels within a macromolecular complex. We present a novel setup consisting of two commercial, state-of-the-art microscopes. A sample scanning atomic force microscope is mounted onto an objective scanning confocal fluorescence lifetime microscope. The ability to move the sample and objective independently allows for precise alignment of AFM probe and laser focus with an accuracy down to a few nanometers. Time correlated single photon counting (TCSPC) gives us the opportunity to measure single-molecule fluorescence lifetimes. We will be able to study molecular complexes in the vicinity of an AFM probe on a level that has yet to be achieved. With this setup we simultaneously obtained single molecule sensitivity in the AFM topography and fluorescence lifetime imaging of YOYO-1 stained lambda-DNA samples and we showed silicon tip induced single molecule quenching on organic fluorophores.

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Fluorescence‐Emission Control of Single CdSe Nanocrystals Using Gold‐Modified AFM Tips

Cited by 27 publications

References 43 publications

Tip induced fluorescence quenching for nanometer optical and topographical resolution

Tip induced fluorescence quenching for nanometer optical and topographical resolution

Tip‐Enhanced Near‐Field Optical Microscopy

Simultaneous single molecule atomic force and fluorescence lifetime imaging

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