Fluorescence endoscopic detection of murine colitis-associated colon cancer by topically applied enzymatically rapid-activatable probe

Mitsunaga, Makoto; Kosaka, Nobuyuki; Choyke, Peter L.; Young, Matthew R.; Dextras, Christopher; Saud, Shakir M.; Colburn, Nancy H.; Sakabe, Masayo; Nagano, Tetsuo; Asanuma, Daisuke; Urano, Yasuteru; Kobayashi, Hisataka

doi:10.1136/gutjnl-2011-301795

Cited by 94 publications

(70 citation statements)

References 34 publications

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“…Recently, GGT expression has also been used for the visualization of the leakage of pancreatic juice using gGlu-HMRG [19]. GGT has been reported to be expressed at higher levels in various cancer cells than in normal cells or in inflammation [13,20]. In the present study, AsPC-1 and PANC-1 cells expressed GGT, but KP4 cells did not.…”

Section: Discussionmentioning

confidence: 42%

“…We have previously reported a rapid-cancer detection method involving topical spraying of a γ-glutamyltranspeptidase (GGT)-activated fluorescent probe, γ-glutamyl hydroxymethyl rhodamine green (gGlu-HMRG) in vivo; this probe becomes fluorescent after cleavage of a GGT-specific sequence [12]. The detection of colon cancer using fluorescence after topical administration (spraying) of gGlu-HMRG has been reported in mice and resected human specimens [13,14].…”

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confidence: 99%

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Feasibility of Using an Enzymatically Activatable Fluorescence Probe for the Rapid Evaluation of Pancreatic Tissue Obtained Using Endoscopic Ultrasound-Guided Fine Needle Aspiration: a Pilot Study

et al. 2015

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Purpose: Endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) is the most reliable method for the histological diagnosis of pancreatic tumors. Rapid on-site fluorescence-guided histological diagnosis was evaluated by topically applying an enzymatically activatable probe onto the EUS-FNA samples; the probe fluoresces in the presence of γ-glutamyltranspeptidase.Procedures: EUS-FNA was performed in 10 pancreatic tumors. After topical application of the probe, signal intensity was measured using a fluorescence imaging system for 13 min. Conclusions: Application of enzymatically activatable probe onto EUS-FNA samples was feasible for the rapid evaluation of adequate tissues for histological diagnosis.

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Section: Discussionmentioning

confidence: 42%

mentioning

confidence: 99%

Feasibility of Using an Enzymatically Activatable Fluorescence Probe for the Rapid Evaluation of Pancreatic Tissue Obtained Using Endoscopic Ultrasound-Guided Fine Needle Aspiration: a Pilot Study

et al. 2015

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“…Tumors even smaller than 1 mm could be discriminated from normal mammary gland tissue (12). In mouse models for colon cancer and disseminated peritoneal ovarian cancer, tumors could be clearly visualized 1 min after topical administration (11,13).…”

Section: Target Prioritization For Theranostic Approaches In Pda Basementioning

confidence: 85%

Data-Driven Prioritization and Review of Targets for Molecular-Based Theranostic Approaches in Pancreatic Cancer

et al. 2017

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Molecularly targeted therapeutic and imaging strategies directed at aberrant signaling pathways in pancreatic tumor cells may improve the poor outcome of pancreatic ductal adenocarcinoma (PDA). Therefore, relevant molecular targets need to be identified. Methods: We collected publicly available expression profiles of patient-derived normal pancreatic tissue (n 5 77) and PDA samples (n 5 103). Functional genomic messenger RNA profiling was applied to predict target upregulation on the protein level. We prioritized these targets based on current status of preclinical therapeutic and imaging evaluation in PDA. Results: We identified 213 significantly upregulated proteins in PDA compared with normal pancreatic tissue. We prioritized mucin-1, mesothelin, g-glutamyltransferase 5, and cathepsin-E as the most interesting targets, because studies already demonstrated their potential for both therapeutic and imaging strategies in literature. Conclusion: This study can assist clinicians and drug developers in deciding which theranostic targets should be taken for further clinical evaluation in PDA.

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“…Their probe showed relatively higher T/B ratio of 5.70 ± 2.30 than our T/B ratio of 1.78 ± 0.18 (MMP9) and 2.10 ± 0.28 (MMP14) as their activatable probe could minimize background signal though there exists potential toxicity issue [32]. Lately, a topically applied enzymatically activatable probe targeting cancer associated enzyme showed rapid detection and high target-to-background in murine colon cancer model [33]. The use of QDs have several unique advantages allowing multiple colors in visible spectrum range and can be extended to more number of channels in the near-infrared window with only single excitation source due to relatively narrow and symmetric emission spectrum.…”

Section: Discussionmentioning

confidence: 99%

“…All ROI intensities were recorded in pixel intensity values between 0 and 255. After obtaining mean signal intensities, the T/B ratio was determined as S t /S n [22]. Data are presented as means ± standard deviation of the mean, and each signal intensity value represents either tumor or normal mucosa signal intensity subtracted by background signal intensity (intrinsic camera noise).…”

Section: In Vivo Molecular Fluorescence Imaging Of Colonic Tumorsmentioning

confidence: 99%

Intravital imaging of mouse colonic adenoma using MMP-based molecular probes with multi-channel fluorescence endoscopy

Yoo

Jung

et al. 2014

Biomed. Opt. Express

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Abstract:Intravital imaging has provided molecular, cellular and anatomical insight into the study of tumor. Early detection and treatment of gastrointestinal (GI) diseases can be enhanced with specific molecular markers and endoscopic imaging modalities. We present a wide-field multichannel fluorescence endoscope to screen GI tract for colon cancer using multiple molecular probes targeting matrix metalloproteinases (MMP) conjugated with quantum dots (QD) in AOM/DSS mouse model. MMP9 and MMP14 antibody (Ab)-QD conjugates demonstrate specific binding to colonic adenoma. The average target-to-background (T/B) ratios are 2.10 ± 0.28 and 1.78 ± 0.18 for MMP14 Ab-QD and MMP9 Ab-QD, respectively. The overlap between the two molecular probes is 67.7 ± 8.4%. The presence of false negative indicates that even more number of targeting could increase the sensitivity of overall detection given heterogeneous molecular expression in tumors. Our approach indicates potential for the screening of small or flat lesions that are precancerous. References and links1. G. Oh, E. Chung, and S. H. Yun, "Optical fibers for high-resolution in vivo microendoscopic fluorescence imaging," Opt. Fiber Technol. 19(6), 760-771 (2013). 2. J. C. van Rijn, J. B. Reitsma, J. Stoker, P. M. Bossuyt, S. J. van Deventer, and E. Dekker, "Polyp miss rate determined by tandem colonoscopy: a systematic review," Am. J. Gastroenterol. 101(2), 343-350 (2006). 3. C. A. Munroe, P. Lee, A. Copland, K. K. Wu, T. Kaltenbach, R. M. Soetikno, and S. Friedland, "A tandem colonoscopy study of adenoma miss rates during endoscopic training: a venture into uncharted territory," Gastrointest. Endosc. 75(3), 561-567 (2012 Rat, P. Roignot, J. Faivre, P. Laurent-Puig, and F. Piard, "Mutations in the RAS-MAPK, PI(3)K (phosphatidylinositol-3-OH kinase) signaling network correlate with poor survival in a population-based series of colon cancers," Int.

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Fluorescence endoscopic detection of murine colitis-associated colon cancer by topically applied enzymatically rapid-activatable probe

Abstract: These results suggest that gGlu-HMRG can improve endoscopic detection of CAC with a higher target to background ratio than conventional white light colonoscopy. This could be of benefit to patients with long-standing colitis who must undergo repeated screening colonoscopies.

Cited by 94 publications

References 34 publications

Feasibility of Using an Enzymatically Activatable Fluorescence Probe for the Rapid Evaluation of Pancreatic Tissue Obtained Using Endoscopic Ultrasound-Guided Fine Needle Aspiration: a Pilot Study

Feasibility of Using an Enzymatically Activatable Fluorescence Probe for the Rapid Evaluation of Pancreatic Tissue Obtained Using Endoscopic Ultrasound-Guided Fine Needle Aspiration: a Pilot Study

Data-Driven Prioritization and Review of Targets for Molecular-Based Theranostic Approaches in Pancreatic Cancer

Intravital imaging of mouse colonic adenoma using MMP-based molecular probes with multi-channel fluorescence endoscopy

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