Fluorescence enhancement of small squaraine dye and its two-photon excited fluorescence in long-term near-infrared I&amp;II bioimaging

Yi, Rongxing; Das, Pintu; Shen, Binglin; Yang, Zhigang; Zhao, Yihua; Liang, Hong; He, Ying; Hu, Rui; Song, Jun; Qu, Junle; Liu, Liwei

doi:10.1364/oe.27.012360

Cited by 23 publications

(20 citation statements)

References 31 publications

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“…Previously, we have synthesized a small squaraine dye (SD), which exhibited excellent twophoton emission when interacting with BSA [27]. In the present study, we demonstrated that this SD can selectively detect BSA on the basis of the changes in the proportion of a relatively longer lifetime component.…”

Section: Introductionmentioning

confidence: 62%

“…Mito-G, Rho-123 and BSA-Alexa Fluor 488 fluorescence was excited at 488 nm, and the emission was collected from 500 to 550 nm. SD fluorescence was excited using a 561 nm laser, and the emission was collected from 570 to 620 nm, which matched well with the spectra range of SD that we have previously reported [27]. Colocalization analyses of two fluorescence channels were conducted using the Nikon software NIS-Elements AR.…”

Section: Confocal Laser Scanning Microscopy (Clsm)mentioning

confidence: 95%

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Monitoring the endocytosis of bovine serum albumin based on the fluorescence lifetime of small squaraine dye in living cells

Das

Zhao

Shen

et al. 2019

Biomed. Opt. Express

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Bovine serum albumin (BSA) has a wide range of physiological functions involving the binding, transportation, and delivery of fatty acids, porphyrins, bilirubin, steroids, etc. In the present study, we prepared a small squaraine dye (SD), which can selectively detect BSA using fluorescence lifetime imaging microscopy (FLIM), to monitor the endocytosis of BSA in live cultured cells in real time. This approach revealed that BSA uptake is concentration-dependent in living cells. Furthermore, we used paclitaxel (PTX), a chemotherapeutic drug, to influence the endocytosis of BSA in living cells. The results demonstrated that the endocytic rate was clearly reduced after pretreatment with 0.4 µM PTX for 2 h. The present study demonstrates the potential value of using the fluorescence lifetime of SD to detect BSA concentration and study the physiological mechanism of chemotherapeutic drugs.

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Section: Introductionmentioning

confidence: 62%

Section: Confocal Laser Scanning Microscopy (Clsm)mentioning

confidence: 95%

Monitoring the endocytosis of bovine serum albumin based on the fluorescence lifetime of small squaraine dye in living cells

Das

Zhao

Shen

et al. 2019

Biomed. Opt. Express

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“… 23 , 24 , 28 − 36 Owing to the resonance enhancement effect, 2P S 0 -to-S n transition is often stronger than S 0 -to-S 1 transition. 25 However, the benefits of NIR-II excitation, such as increased penetration depth 15 and reduced photobleaching, 23 , 30 will outweigh this disadvantage. Although it is a persistent effort goal to improve the absorption cross section, there are other factors—such as quantum yield, photostability, molecular size, functionality, and modification flexibility—that also need to be considered when selecting a fluorophore for 2P applications.…”

Section: Nir-ii Nonlinear Excitation For Deep-tissue Bioimagingmentioning

confidence: 99%

Molecular Fluorophores for Deep-Tissue Bioimaging

2020

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Fluorescence imaging has made tremendous inroads toward understanding the complexity of biological systems, but in vivo deep-tissue imaging remains a great challenge due to the optical opacity of biological tissue. Recent improvements in laser and detector manufacturing have allowed the expansion of nonlinear and linear fluorescence imaging to the underexplored “tissue-transparent” second near-infrared (NIR-II; 1000–1700 nm) window, opening up new opportunities for optical access deep inside opaque tissue. Molecular fluorophores have historically played a major role in fluorescence bioimaging. It is increasingly important to design new molecular fluorophores to fully unlock the potential of NIR-II imaging techniques. In this outlook, we give an overview of the novel molecular fluorophores developed for deep-tissue bioimaging in the past five years and discuss their pros and cons in applications. Guidelines for designing new molecular fluorophores with the desirable properties are also provided.

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“…Yi et al developed a small squarine dye with a fluorescence lifetime that changes depending on serum albumin concentrations. 33 Recent work in human ovarian cancer biopsies by Lin et al 34 shows that this dye is sensitive to concentrations of human serum albumin (HSA), which is a protein that is often concentrated near solid tumors [Fig. 3(b), left panel].…”

Section: Flim Of Exogenous Molecular Probesmentioning

confidence: 99%

Recent innovations in fluorescence lifetime imaging microscopy for biology and medicine

et al. 2021

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Significance: Fluorescence lifetime imaging microscopy (FLIM) measures the decay rate of fluorophores, thus providing insights into molecular interactions. FLIM is a powerful molecular imaging technique that is widely used in biology and medicine.Aim: This perspective highlights some of the major advances in FLIM instrumentation, analysis, and biological and clinical applications that we have found impactful over the last year.Approach: Innovations in FLIM instrumentation resulted in faster acquisition speeds, rapid imaging over large fields of view, and integration with complementary modalities such as singlemolecule microscopy or light-sheet microscopy. There were significant developments in FLIM analysis with machine learning approaches to enhance processing speeds, fit-free techniques to analyze images without a priori knowledge, and open-source analysis resources. The advantages and limitations of these recent instrumentation and analysis techniques are summarized. Finally, applications of FLIM in the last year include label-free imaging in biology, ophthalmology, and intraoperative imaging, FLIM of new fluorescent probes, and lifetime-based Förster resonance energy transfer measurements.Conclusions: A large number of high-quality publications over the last year signifies the growing interest in FLIM and ensures continued technological improvements and expanding applications in biomedical research.

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Fluorescence enhancement of small squaraine dye and its two-photon excited fluorescence in long-term near-infrared I&II bioimaging

Cited by 23 publications

References 31 publications

Monitoring the endocytosis of bovine serum albumin based on the fluorescence lifetime of small squaraine dye in living cells

Monitoring the endocytosis of bovine serum albumin based on the fluorescence lifetime of small squaraine dye in living cells

Molecular Fluorophores for Deep-Tissue Bioimaging

Recent innovations in fluorescence lifetime imaging microscopy for biology and medicine

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