Fluorescence excitation–emission matrix spectroscopy with regional integration analysis for characterizing composition and transformation of dissolved organic matter in landfill leachates

He, Xiao-Song; Xi, Beidou; Wei, Zimin; Jiang, Yonghai; Yang, Yu; An, Da; Cao, Jianhua; Liu, Hongliang

doi:10.1016/j.jhazmat.2011.03.047

Cited by 198 publications

(39 citation statements)

References 25 publications

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“…The volumetric integration under the EEM within each region, normalized to the projected excitation-emission area within that region and to the DOC concentration, resulted in a normalized region-specific EEM volume (P i,n ) (Chen et al, 2003). P h /P p was used to indicate how easily the organic matter could be used by microbes, with lower P h /P p ratios corresponding to easier use (He et al, 2011). Furthermore, P h represents the sum of P i, III and P i,V, and P p represent for the sum of P i,I , P i,II , and P i,IV .…”

Section: Fluorescence Spectroscopymentioning

confidence: 99%

Nitrogen removal processes in deep subsurface wastewater infiltration systems

Zhang

Wang

et al. 2015

Ecological Engineering

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Section: Fluorescence Spectroscopymentioning

confidence: 99%

Nitrogen removal processes in deep subsurface wastewater infiltration systems

Zhang

Wang

et al. 2015

Ecological Engineering

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“…However, we found that component 4 showed a relatively low change in the F max values. Component 4 had a complex structure and a higher degree of conjugation than those of the other components, making it difficult to degrade by microorganisms [46]. Protein-like component 5 reflected the change in microbial activities, with unstable F max values.…”

Section: Eem-parafac Components Of Effluent Dommentioning

confidence: 99%

Characterization of Dissolved Organic Matter from Effluents in a Dry Anaerobic Digestion Process Using Spectroscopic Techniques and Multivariate Statistical Analysis

Guo

Chen

et al. 2016

Waste Biomass Valor

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Dissolved organic matter (DOM) collected from a garage-type dry fermentation system was investigated using UV-Vis absorption and fluorescence spectroscopy. Spectral parameters A 253/203 , biological index and fluorescence index were used to assess the structural characteristics and sources of effluent DOM. The results showed that aromatic rings highly substituted with hydroxyl, carbonyl, ester and carboxylic groups were obtained in the aerobic decomposition stage, and microbially derived organic matter dominated in the dry anaerobic digestion process, suggesting that the hydrolysis of organic macromolecules (lignin) in the aerobic decomposition resulted in the formation of water-soluble intermediates which can be easily assimilated and metabolized by microbial cells in dry anaerobic digestion process. PARAFAC of the excitation-emission matrix spectra revealed five fluorescent components occurring in effluent DOM: four humic-like components (C1-C4) and one protein-like component (C5). The F max values of components 1, 2, 3 and 5 showed large fluctuation than that of component C4, suggested the formation and degradation of these components. Component C4, with high aromatic content and higher degree of humification, was more difficult to be utilized and degraded by microorganisms than other components. The PAR-AFAC-PCA displayed three PCA factors (factors 1, 2 and 3), which accounted for 34.51, 34.20 and 31.11 %, respectively, of the variance in fluorescent components. Humic-like component C4 showed positive factor 1 loadings. Factor 2 was mainly explained by the tryptophan-like component C5. The humic-like components 1, 2 and 3 concurrently showed a relatively high factor 3 loading. The PARAFAC-PCA in this study could separate the characteristics of the DOM fluorescent component namely the source strength of the humic-like components versus the contributions of protein-like components in the total fluorescence analyses.

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“…The speed in the scanning monochromator was about 1200 nm.s -1 and the fluorescence values were recorded every 0.5 nm between 200 and 600 nm. Based on Chen et al (2003), He et al (2013He et al ( , 2011 and Muller et al (2014), the obtained spectra were decomposed on seven zones (zone 1 to 7) according to the fluorescence of each biochemical molecules. The proportion of fluorescence of a zone "i" (P (iሻሻ was calculated from the fluorescence zone volume (equations 1 and 2).…”

Section: Fluorescence Spectroscopy Analysismentioning

confidence: 99%