Fluorescence excitation on monolithically integrated all-polymer chips

Schelb, Mauno; Vannahme, Christoph; Welle, Alexander; Lenhert, Steven; Ross, Benjamin; Mappes, Timo

doi:10.1117/1.3446672

Cited by 7 publications

(4 citation statements)

References 18 publications

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“…6b. This can be assigned to an exponential decay of the laser light intensity by absorption from the marker molecules and is consistent with results achieved with external lasers [27]. …”

Section: Measurement Resultssupporting

confidence: 65%

Plastic lab-on-a-chip for fluorescence excitation with integrated organic semiconductor lasers

Vannahme¹,

Klinkhammer²,

Lemmer³

et al. 2011

Opt. Express

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Laser light excitation of fluorescent markers offers highly sensitive and specific analysis for bio-medical or chemical analysis. To profit from these advantages for applications in the field or at the point-ofcare, a plastic lab-on-a-chip with integrated organic semiconductor lasers is presented here. First order distributed feedback lasers based on the organic semiconductor tris(8-hydroxyquinoline) aluminum (Alq 3 ) doped with the laser dye 4-dicyanomethylene-2-methyl-6-(p-dimethylaminostyril)-4H-pyrane (DCM), deep ultraviolet induced waveguides, and a nanostructured microfluidic channel are integrated into a poly(methyl methacrylate) (PMMA) substrate. A simple and parallel fabrication process is used comprising thermal imprint, DUV exposure, evaporation of the laser material, and sealing by thermal bonding. The excitation of two fluorescent marker model systems including labeled antibodies with light emitted by integrated lasers is demonstrated.

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“…6b. This can be assigned to an exponential decay of the laser light intensity by absorption from the marker molecules and is consistent with results achieved with external lasers [27]. …”

Section: Measurement Resultssupporting

confidence: 65%

Plastic lab-on-a-chip for fluorescence excitation with integrated organic semiconductor lasers

Vannahme¹,

Klinkhammer²,

Lemmer³

et al. 2011

Opt. Express

Self Cite

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“…developed an all‐polymer chip with integrated optical waveguides and microfluidic channels. [ 69 ] This paper described a relatively simple method to fabricate the device. The microfluidic channels were formed using deep UV photochemical degrade, followed by chemical etching on PMMA material.…”

Section: Optofluidic Cell Analysis For Biodiagnostic Applicationsmentioning

confidence: 99%

Emerging optofluidic technologies for biodiagnostic applications

Dai

et al. 2021

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The unprecedented global COVID‐19 pandemic strongly argues the critical need for innovative diagnostic tools meeting the requirement of test speed, accuracy, and throughput. Owing to the integration of optics and microfluidics technologies, optofluidic technology enables highly precise flow manipulation and highly sensitive signal detection at the microscale, thus offering the promising potential for developing biodiagnostic applications. Research toward this direction is fast growing into an emerging area. In this paper, we give an overview of emerging optofluidic technologies for biodiagnostic applications with the focus on three common types of biomarkers: nucleic acid, protein, and cell. We conclude by discussing the challenges, opportunities, and future perspectives of this field.

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“…There are more optic-fluid integration examples for POC cytometry. Schelb et al 74 monolithically integrated a polymer waveguide with a microfluidic channel to excite flowing cells only located on the tip of the waveguide by the evanescent field. The demonstrated ease of handling waveguide-cell alignment in the microfluidic channel may open a way for advancing POC technology.…”

Section: Fluorescence Detection-based Optofluidic Devicesmentioning

confidence: 99%

Optofluidic detection for cellular phenotyping

Tung

Huang

et al. 2012

Lab Chip

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Quantitative analysis of the output of processes and molecular interactions within a single cell is highly critical to the advancement of accurate disease screening and personalized medicine. Optical detection is one of the most broadly adapted measurement methods in biological and clinical assays and serves cellular phenotyping. Recently, microfluidics has obtained increasing attention due to several advantages, such as small sample and reagent volumes, very high throughput, and accurate flow control in the spatial and temporal domains. Optofluidics, which is the attempt to integrate optics with microfluidic, shows great promise to enable on-chip phenotypic measurements with high precision, sensitivity, specificity, and simplicity. This paper reviews the most recent developments of optofluidic technologies for cellular phenotyping optical detection.

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Fluorescence excitation on monolithically integrated all-polymer chips

Cited by 7 publications

References 18 publications

Plastic lab-on-a-chip for fluorescence excitation with integrated organic semiconductor lasers

Plastic lab-on-a-chip for fluorescence excitation with integrated organic semiconductor lasers

Emerging optofluidic technologies for biodiagnostic applications

Optofluidic detection for cellular phenotyping

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