Fluorescence Imaging for Biomedical Analysis

Abstract

Fluorescence imaging techniques became powerful tools for inquisitive biologists and medical doctors. During the past decade, the description of new imaging modalities such as fluorescence life‐time, confocal, fluorescence anisotropy, and two‐photon microscopy has triggered an explosion in fluorescence imaging for biomedical analysis techniques. This article describes the basic principle of fluorescence imaging techniques and elaborates about their biomedical analysis applications

Fluorescence in neurosurgery: Its therapeutic and diagnostic significance - a comprehensive review

“…The principle of fluorescence imaging is based on the interaction between light and fluorescent molecules, known as fluorophores 3 , to visualize and study biological or chemical systems at a cellular or molecular level. It involves the excitation of fluorophores within a sample using specific wavelengths of light, followed by detecting the emitted fluorescence at different wavelengths 4 , 5 .…”