Fluorescence Imaging of Inflammation with Optical Probes

Abstract

Inflammation plays an important role in the occurrence and development of disease; dysregulation of inflammatory progression often leads to disease such as tissue sclerosis, cancers, stroke, etc. Optical imaging technology, due to its higher sensitivity and resolution, can provide finer images for the observation of inflammation. Many optical probes have been developed as contrast agents for optical imaging techniques in different diseases. In this review, we summarize the recent advances of optical probe and imaging methods for imaging inflammation in different organs, such as brain, liver, lung, kidney, intestine, etc. Finally, we discuss the opportunities and challenges of optical probes used in the clinic for inflammation monitoring and prospect their future development in disease detection.

Fluorescence Imaging Using Deep-Red Indocyanine Blue, a Complementary Partner for Near-Infrared Indocyanine Green

“…We tested this possibility by conducting mouse imaging experiments that monitored changes in blood perfusion induced by inflammation. In recent years, fluorescence imaging has emerged as a valuable tool for diagnosing inflammatory diseases and conditions. − Inflammation is associated with changes in micro vascularization and blood vessel permeability, leading to increased accumulation of blood proteins within inflamed tissue. We employed a standard mouse inflammation model that injected lipopolysaccharide (LPS) into a mouse paw and used in vivo fluorescence imaging to visualize blood flow compared to the contralateral paw .…”

Dynamic monitoring of the fibrosis disease by a collagen targeting near infrared probe

“…Compared with these imaging techniques, fluorescence imaging has the advantages of high resolution and sensitivity, low cost, real-time performance, and good compatibility. 24 Herein, by combining a collagen targeting peptide with a H 2 O 2 responsive probe, we have developed a water soluble, biocompatible and NIR-emitting optical probe PCyPB for the non-invasive and sensitive imaging of collagen in fibrotic tissue. At the cell level, PCyPB has a specific response to the endogenous or exogenous H 2 O 2 and a good ability to bind collagen.…”

Chronological development of functional fluorophores for bio-imaging

“…12–14 These techniques further detect disease markers for early diagnosis, including cancer, cardiovascular diseases, and neurodegenerative disorders. 15–20 This convergence of imaging modalities holds immense promise for the future of precision diagnosis.…”